University proceedings. Volga region. Natural sciences
Background. A domestic dog (Canis familiaris) is one of the most common domestic animals, the diversity of their ecological and social status is determined by their reproduction and reproduction in cities and rural settlements. In populated areas, dogs have a significant impact on the fauna of th...
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| Format: | Article |
| Language: | English |
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Penza State University Publishing House
2024-11-01
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| Series: | Известия высших учебных заведений. Поволжский регион: Естественные науки |
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| _version_ | 1832541239260479488 |
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| author | A.V. Kuzmin |
| author_facet | A.V. Kuzmin |
| author_sort | A.V. Kuzmin |
| collection | DOAJ |
| description | Background. A domestic dog (Canis familiaris) is one of the most common domestic
animals, the diversity of their ecological and social status is determined by their reproduction
and reproduction in cities and rural settlements. In populated areas, dogs have a
significant impact on the fauna of the ecosystem. The purpose of the study was to analyze
the genetic structure of Penza stray dog population based on the study of individual polymorphism
by microsatellite loci of nuclear DNA. Materials and methods. The material for the
study was hair samples of domestic and stray dogs collected in the period 2021–2024 in
shelters and centers for overexposure of stray animals, on the streets of Penza and provided
by pet dog owners. To isolate DNA, 23 wool samples were collected, of which 11 were obtained
from stray dogs and 12 from domestic dogs. To amplify microsatellite DNA fragments
consisting of tri- and tetra tandem repeats, the primer systems of the Stock Marks Dog Genotyping
Kit identification panel of the American Kennel Association were used. Results. The
approbation of 6 microsatellite systems (PEZ3 F/R, PEZ6 F/R, PEZ8 F/R, FHC2010 F/R,
FHC2045 F/R, FHC2079 F/R) showed that they are extremely nonspecific for the DNA matrix
obtained from dogs living in the territory of the Russian Federation. Apparently, the genetic
lines of the domestic dog of the North American continent are quite different from
domestic lines. The obtained microsatellite allelic spectra of DNA samples from stray and
domestic dogs are mainly represented by heterozygous sets. At the same time, heterozygosity
was slightly higher in the population of stray dogs (0.750) compared with the heterozygosity
of domestic dogs (0.670). Conclusions. The performed procedure for DNA extraction from
hair samples of domestic and stray dogs revealed its low productivity. For a successful study
of the genetic structure of the population of stray animals, more productive methods should
be used, for example, tissue biopsy. The results of amplification of marker fragments of microsatellite
DNA of stray dogs showed poor performance of the tested microsatellite systems
of the dog identification panel of the American Kennel Association. It is necessary to start
developing its own panel of microsatellite markers suitable for the identification of dogs of
domestic cultural breeding. |
| format | Article |
| id | doaj-art-a2fae3a7b66243f38a6fdf80e3bef937 |
| institution | Kabale University |
| issn | 2307-9150 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Penza State University Publishing House |
| record_format | Article |
| series | Известия высших учебных заведений. Поволжский регион: Естественные науки |
| spelling | doaj-art-a2fae3a7b66243f38a6fdf80e3bef9372025-02-04T09:13:42ZengPenza State University Publishing HouseИзвестия высших учебных заведений. Поволжский регион: Естественные науки2307-91502024-11-013334310.21685/2307-9150-2024-3-4University proceedings. Volga region. Natural sciencesA.V. Kuzmin0Penza State UniversityBackground. A domestic dog (Canis familiaris) is one of the most common domestic animals, the diversity of their ecological and social status is determined by their reproduction and reproduction in cities and rural settlements. In populated areas, dogs have a significant impact on the fauna of the ecosystem. The purpose of the study was to analyze the genetic structure of Penza stray dog population based on the study of individual polymorphism by microsatellite loci of nuclear DNA. Materials and methods. The material for the study was hair samples of domestic and stray dogs collected in the period 2021–2024 in shelters and centers for overexposure of stray animals, on the streets of Penza and provided by pet dog owners. To isolate DNA, 23 wool samples were collected, of which 11 were obtained from stray dogs and 12 from domestic dogs. To amplify microsatellite DNA fragments consisting of tri- and tetra tandem repeats, the primer systems of the Stock Marks Dog Genotyping Kit identification panel of the American Kennel Association were used. Results. The approbation of 6 microsatellite systems (PEZ3 F/R, PEZ6 F/R, PEZ8 F/R, FHC2010 F/R, FHC2045 F/R, FHC2079 F/R) showed that they are extremely nonspecific for the DNA matrix obtained from dogs living in the territory of the Russian Federation. Apparently, the genetic lines of the domestic dog of the North American continent are quite different from domestic lines. The obtained microsatellite allelic spectra of DNA samples from stray and domestic dogs are mainly represented by heterozygous sets. At the same time, heterozygosity was slightly higher in the population of stray dogs (0.750) compared with the heterozygosity of domestic dogs (0.670). Conclusions. The performed procedure for DNA extraction from hair samples of domestic and stray dogs revealed its low productivity. For a successful study of the genetic structure of the population of stray animals, more productive methods should be used, for example, tissue biopsy. The results of amplification of marker fragments of microsatellite DNA of stray dogs showed poor performance of the tested microsatellite systems of the dog identification panel of the American Kennel Association. It is necessary to start developing its own panel of microsatellite markers suitable for the identification of dogs of domestic cultural breeding.canis familiarisstray animalspopulation polymorphismmicrosatellites |
| spellingShingle | A.V. Kuzmin University proceedings. Volga region. Natural sciences Известия высших учебных заведений. Поволжский регион: Естественные науки canis familiaris stray animals population polymorphism microsatellites |
| title | University proceedings. Volga region. Natural sciences |
| title_full | University proceedings. Volga region. Natural sciences |
| title_fullStr | University proceedings. Volga region. Natural sciences |
| title_full_unstemmed | University proceedings. Volga region. Natural sciences |
| title_short | University proceedings. Volga region. Natural sciences |
| title_sort | university proceedings volga region natural sciences |
| topic | canis familiaris stray animals population polymorphism microsatellites |
| work_keys_str_mv | AT avkuzmin universityproceedingsvolgaregionnaturalsciences |