Rapid, highly specific and sensitive detection of human adenovirus using multiple cross displacement amplification coupled with CRISPR-Cas12a-based detection

Rapid, highly specific and sensitive detection of human adenovirus using multiple cross displacement amplification coupled with CRISPR-Cas12a-based detection

Human adenoviruses (HAdV), particularly serotypes 3 and 7, are significant pathogens that cause respiratory tract infection. Here, we developed a novel HAdV diagnostic assay, termed HAdV-MCDA-CRISPR, by combining multiple cross displacement amplification (MCDA) with CRISPR/Cas12a-based detection. HA...

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Bibliographic Details
Main Authors: Linglong Wan, Juan Zhou, Lei Yu, Xiaolan Huang, Jin Fu, Fei Xiao, Nan Jia, Yu Zhang, Min Chen, Xu Chen, Zhaomin Feng, Yi Wang, Lihui Meng
Format: Article
Language:English
Published: Elsevier 2025-08-01
Series:Talanta Open
Subjects:
Human adenoviruses
Multiple cross displacement amplification
CRISPR, Cas12a, crRNA
Online Access:http://www.sciencedirect.com/science/article/pii/S2666831925000645
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