Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure
Summary: Contrast transfer function (CTF) estimation is essential to the data processing workflow of cryo-electron tomography (cryoET). Here, we present a protocol for CTF estimation of the cryoET tilt series with CTFMeasure. CTFMeasure can estimate the CTF parameters together with the absolute tilt...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007159 |
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| author | Ranhao Zhang Yuan Shen Xueming Li |
| author_facet | Ranhao Zhang Yuan Shen Xueming Li |
| author_sort | Ranhao Zhang |
| collection | DOAJ |
| description | Summary: Contrast transfer function (CTF) estimation is essential to the data processing workflow of cryo-electron tomography (cryoET). Here, we present a protocol for CTF estimation of the cryoET tilt series with CTFMeasure. CTFMeasure can estimate the CTF parameters together with the absolute tilt angle offset of the sample. We describe steps for configuring the input files and estimating the CTF parameters and the absolute tilt angle offset of the input tilt series. We then detail procedures for visualizing the power spectra and analyzing the output files.For complete details on the use and execution of this protocol, please refer to Zhang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-a2387d8ff1be4eb7bd092b107bf8c608 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-a2387d8ff1be4eb7bd092b107bf8c6082025-01-24T04:45:43ZengElsevierSTAR Protocols2666-16672025-03-0161103550Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasureRanhao Zhang0Yuan Shen1Xueming Li2Key Laboratory for Protein Sciences of Ministry of Education, School of Life Sciences, Tsinghua University, Beijing 100084, China; State Key Laboratory of Membrane Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Joint Center for Life Sciences, Beijing 100084, China; Beijing Frontier Research Center for Biological Structure, Beijing 100084, China; School of Life Sciences, Tsinghua University, Beijing 100084, China; Department of Electronic Engineering, Tsinghua University, Beijing 100084, China; Beijing National Research Center for Information Science and Technology, Tsinghua University, Beijing 100084, ChinaDepartment of Electronic Engineering, Tsinghua University, Beijing 100084, China; Beijing National Research Center for Information Science and Technology, Tsinghua University, Beijing 100084, China; Corresponding authorKey Laboratory for Protein Sciences of Ministry of Education, School of Life Sciences, Tsinghua University, Beijing 100084, China; State Key Laboratory of Membrane Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Joint Center for Life Sciences, Beijing 100084, China; Beijing Frontier Research Center for Biological Structure, Beijing 100084, China; School of Life Sciences, Tsinghua University, Beijing 100084, China; Corresponding authorSummary: Contrast transfer function (CTF) estimation is essential to the data processing workflow of cryo-electron tomography (cryoET). Here, we present a protocol for CTF estimation of the cryoET tilt series with CTFMeasure. CTFMeasure can estimate the CTF parameters together with the absolute tilt angle offset of the sample. We describe steps for configuring the input files and estimating the CTF parameters and the absolute tilt angle offset of the input tilt series. We then detail procedures for visualizing the power spectra and analyzing the output files.For complete details on the use and execution of this protocol, please refer to Zhang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724007159Cell BiologyMicroscopyCryo-EM |
| spellingShingle | Ranhao Zhang Yuan Shen Xueming Li Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure STAR Protocols Cell Biology Microscopy Cryo-EM |
| title | Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure |
| title_full | Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure |
| title_fullStr | Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure |
| title_full_unstemmed | Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure |
| title_short | Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure |
| title_sort | protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo electron tomography using ctfmeasure |
| topic | Cell Biology Microscopy Cryo-EM |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724007159 |
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