Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model
Background. Interactions between alcohol, infection, and surgery and their effect on differentiation and functionality of T helper cells are not yet completely understood. We hypothesized that alcohol and surgery disturb differentiation of T helper cells and contribute to an impaired immune response...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | International Journal of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/1067598 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561724841000960 |
|---|---|
| author | Nadine Lanzke Mario Menk Clarissa von Haefen Lilit Sargsyan Bianca Scharf Klaus-Dieter Wernecke Claudia D. Spies |
| author_facet | Nadine Lanzke Mario Menk Clarissa von Haefen Lilit Sargsyan Bianca Scharf Klaus-Dieter Wernecke Claudia D. Spies |
| author_sort | Nadine Lanzke |
| collection | DOAJ |
| description | Background. Interactions between alcohol, infection, and surgery and their effect on differentiation and functionality of T helper cells are not yet completely understood. We hypothesized that alcohol and surgery disturb differentiation of T helper cells and contribute to an impaired immune response. Methods. Mice were treated with alcohol for two weeks. Saline treatment served as control. Clinical performance and weight were assessed. On day 14, a median laparotomy was performed and animals were challenged with Klebsiella pneumoniae intranasally. Bacterial load was determined in lungs and blood. T helper cell subpopulations and the released cytokines were assessed in lungs, spleens, and plasma. Key transcription factors of T cell differentiation were evaluated. Results. Alcohol significantly impaired clinical appearance and body weight of animals with postsurgical infection (p<0.05). Bacterial load was significantly higher after alcohol treatment (p<0.05). T helper cell subsets and released cytokine levels were significantly altered in lung, but not in spleen. Expression of transcription factors of T helper cell lineage commitment did not translate into different counts of T helper cells. Conclusions. Alcohol and surgery lead to significant cellular and functional modulations of T helper cells during postsurgical infection. These effects may contribute to an impaired immune response after surgery. |
| format | Article |
| id | doaj-art-a1c34bf4674145979d67cc8aeba54a4d |
| institution | Kabale University |
| issn | 2090-8040 2042-0099 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Inflammation |
| spelling | doaj-art-a1c34bf4674145979d67cc8aeba54a4d2025-02-03T01:24:22ZengWileyInternational Journal of Inflammation2090-80402042-00992017-01-01201710.1155/2017/10675981067598Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection ModelNadine Lanzke0Mario Menk1Clarissa von Haefen2Lilit Sargsyan3Bianca Scharf4Klaus-Dieter Wernecke5Claudia D. Spies6Department of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Charité Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, GermanyDepartment of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Charité Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, GermanyDepartment of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Charité Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, GermanyDepartment of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Charité Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, GermanyDepartment of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Charité Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, GermanySOSTANA GmbH, Wildensteiner Straße 27, 10318 Berlin, GermanyDepartment of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Charité Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, GermanyBackground. Interactions between alcohol, infection, and surgery and their effect on differentiation and functionality of T helper cells are not yet completely understood. We hypothesized that alcohol and surgery disturb differentiation of T helper cells and contribute to an impaired immune response. Methods. Mice were treated with alcohol for two weeks. Saline treatment served as control. Clinical performance and weight were assessed. On day 14, a median laparotomy was performed and animals were challenged with Klebsiella pneumoniae intranasally. Bacterial load was determined in lungs and blood. T helper cell subpopulations and the released cytokines were assessed in lungs, spleens, and plasma. Key transcription factors of T cell differentiation were evaluated. Results. Alcohol significantly impaired clinical appearance and body weight of animals with postsurgical infection (p<0.05). Bacterial load was significantly higher after alcohol treatment (p<0.05). T helper cell subsets and released cytokine levels were significantly altered in lung, but not in spleen. Expression of transcription factors of T helper cell lineage commitment did not translate into different counts of T helper cells. Conclusions. Alcohol and surgery lead to significant cellular and functional modulations of T helper cells during postsurgical infection. These effects may contribute to an impaired immune response after surgery.http://dx.doi.org/10.1155/2017/1067598 |
| spellingShingle | Nadine Lanzke Mario Menk Clarissa von Haefen Lilit Sargsyan Bianca Scharf Klaus-Dieter Wernecke Claudia D. Spies Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model International Journal of Inflammation |
| title | Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model |
| title_full | Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model |
| title_fullStr | Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model |
| title_full_unstemmed | Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model |
| title_short | Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model |
| title_sort | ethanol induced alterations of t cells and cytokines after surgery in a murine infection model |
| url | http://dx.doi.org/10.1155/2017/1067598 |
| work_keys_str_mv | AT nadinelanzke ethanolinducedalterationsoftcellsandcytokinesaftersurgeryinamurineinfectionmodel AT mariomenk ethanolinducedalterationsoftcellsandcytokinesaftersurgeryinamurineinfectionmodel AT clarissavonhaefen ethanolinducedalterationsoftcellsandcytokinesaftersurgeryinamurineinfectionmodel AT lilitsargsyan ethanolinducedalterationsoftcellsandcytokinesaftersurgeryinamurineinfectionmodel AT biancascharf ethanolinducedalterationsoftcellsandcytokinesaftersurgeryinamurineinfectionmodel AT klausdieterwernecke ethanolinducedalterationsoftcellsandcytokinesaftersurgeryinamurineinfectionmodel AT claudiadspies ethanolinducedalterationsoftcellsandcytokinesaftersurgeryinamurineinfectionmodel |