fRFLP and fAFLP: Medium-Throughput Genotyping by Fluorescently Post-Labeling Restriction Digestion
Genome-scale studies of population structure and high-resolution mapping of genetically complex traits both require techniques for accurately and efficiently genotyping large numbers of polymorphic sites in multiple individuals. Many high-throughput genotyping technologies require the purchase of ex...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2002-09-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/02333st04 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850152550866616320 |
|---|---|
| author | Brian P. Lazzaro Bonnielin K. Sceurman Susan L. Carney Andrew G. Clark |
| author_facet | Brian P. Lazzaro Bonnielin K. Sceurman Susan L. Carney Andrew G. Clark |
| author_sort | Brian P. Lazzaro |
| collection | DOAJ |
| description | Genome-scale studies of population structure and high-resolution mapping of genetically complex traits both require techniques for accurately and efficiently genotyping large numbers of polymorphic sites in multiple individuals. Many high-throughput genotyping technologies require the purchase of expensive equipment or consumables and are therefore out of reach of some individual research laboratories. Conversely, less expensive technologies are often labor intensive so that the effort involved in typing large numbers of samples or polymorphic sites is prohibitive. Here we present a method of fluorescently post-labeling restriction digestion using standard dye-terminator sequencing chemistry so that RFLP and AFLP products can be visualized on an automated sequencer. This labeling method is efficient, inexpensive, easily multiplexed, and requires no unusual equipment or reagents, thus striking a balance between cost and throughput that should be appropriate for many research groups and core facilities. |
| format | Article |
| id | doaj-art-a19e0783cd984d0ebafb124b22d9ee68 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2002-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-a19e0783cd984d0ebafb124b22d9ee682025-08-20T02:25:57ZengTaylor & Francis GroupBioTechniques0736-62051940-98182002-09-0133353954610.2144/02333st04fRFLP and fAFLP: Medium-Throughput Genotyping by Fluorescently Post-Labeling Restriction DigestionBrian P. Lazzaro0Bonnielin K. Sceurman1Susan L. Carney2Andrew G. Clark31Pennsylvania State University, University Park, PA, USA1Pennsylvania State University, University Park, PA, USA1Pennsylvania State University, University Park, PA, USA1Pennsylvania State University, University Park, PA, USAGenome-scale studies of population structure and high-resolution mapping of genetically complex traits both require techniques for accurately and efficiently genotyping large numbers of polymorphic sites in multiple individuals. Many high-throughput genotyping technologies require the purchase of expensive equipment or consumables and are therefore out of reach of some individual research laboratories. Conversely, less expensive technologies are often labor intensive so that the effort involved in typing large numbers of samples or polymorphic sites is prohibitive. Here we present a method of fluorescently post-labeling restriction digestion using standard dye-terminator sequencing chemistry so that RFLP and AFLP products can be visualized on an automated sequencer. This labeling method is efficient, inexpensive, easily multiplexed, and requires no unusual equipment or reagents, thus striking a balance between cost and throughput that should be appropriate for many research groups and core facilities.https://www.future-science.com/doi/10.2144/02333st04 |
| spellingShingle | Brian P. Lazzaro Bonnielin K. Sceurman Susan L. Carney Andrew G. Clark fRFLP and fAFLP: Medium-Throughput Genotyping by Fluorescently Post-Labeling Restriction Digestion BioTechniques |
| title | fRFLP and fAFLP: Medium-Throughput Genotyping by Fluorescently Post-Labeling Restriction Digestion |
| title_full | fRFLP and fAFLP: Medium-Throughput Genotyping by Fluorescently Post-Labeling Restriction Digestion |
| title_fullStr | fRFLP and fAFLP: Medium-Throughput Genotyping by Fluorescently Post-Labeling Restriction Digestion |
| title_full_unstemmed | fRFLP and fAFLP: Medium-Throughput Genotyping by Fluorescently Post-Labeling Restriction Digestion |
| title_short | fRFLP and fAFLP: Medium-Throughput Genotyping by Fluorescently Post-Labeling Restriction Digestion |
| title_sort | frflp and faflp medium throughput genotyping by fluorescently post labeling restriction digestion |
| url | https://www.future-science.com/doi/10.2144/02333st04 |
| work_keys_str_mv | AT brianplazzaro frflpandfaflpmediumthroughputgenotypingbyfluorescentlypostlabelingrestrictiondigestion AT bonnielinksceurman frflpandfaflpmediumthroughputgenotypingbyfluorescentlypostlabelingrestrictiondigestion AT susanlcarney frflpandfaflpmediumthroughputgenotypingbyfluorescentlypostlabelingrestrictiondigestion AT andrewgclark frflpandfaflpmediumthroughputgenotypingbyfluorescentlypostlabelingrestrictiondigestion |