Production of Extracellular Laccase from Bacillus subtilis MTCC 2414 Using Agroresidues as a Potential Substrate
Laccases are the model enzymes for multicopper oxidases and participate in several applications such as bioremediation, biopulping, textile, and food industries. Laccase producing bacterium, Bacillus subtilis MTCC 2414, was subjected to optimization by conventional techniques and was partially purif...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2015/765190 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567117197606912 |
|---|---|
| author | Narayanan P. Muthukumarasamy Beenie Jackson Antony Joseph Raj Murugan Sevanan |
| author_facet | Narayanan P. Muthukumarasamy Beenie Jackson Antony Joseph Raj Murugan Sevanan |
| author_sort | Narayanan P. Muthukumarasamy |
| collection | DOAJ |
| description | Laccases are the model enzymes for multicopper oxidases and participate in several applications such as bioremediation, biopulping, textile, and food industries. Laccase producing bacterium, Bacillus subtilis MTCC 2414, was subjected to optimization by conventional techniques and was partially purified using ammonium salt precipitation method. The agroresidue substrates used for higher yield of laccase were rice bran and wheat bran. Maximum production was achieved at temperature 30°C (270 ± 2.78 U/mL), pH 7.0 (345 ± 3.14 U/mL), and 96 h (267 ± 2.64 U/mL) of incubation. The carbon and nitrogen sources resulted in high enzyme yield at 3% sucrose (275 ± 3.11 U/mL) and 3% peptone (352.2 ± 4.32 U/mL) for rice bran and 3% sucrose (247.4 ± 3.51 U/mL) and 3% peptone (328 ± 3.33 U/mL) for wheat bran, respectively. The molecular weights of partially purified laccase were 52 kDa for rice bran and 55 kDa for wheat bran. The laccase exhibited optimal activity at 70°C (260.3 ± 6.15 U/mL), pH 9.0 (266 ± 4.02 U/mL), and metal ion CuSO4 (141.4 ± 6.64) was found to increase the production. This is the first report that delivers the higher yield of laccase produced from B. subtilis MTCC 2414 using agroresidues as a potential substrate. |
| format | Article |
| id | doaj-art-a0fc7d0126d7477ea11344cabfebb6e4 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-a0fc7d0126d7477ea11344cabfebb6e42025-02-03T01:02:16ZengWileyBiochemistry Research International2090-22472090-22552015-01-01201510.1155/2015/765190765190Production of Extracellular Laccase from Bacillus subtilis MTCC 2414 Using Agroresidues as a Potential SubstrateNarayanan P. Muthukumarasamy0Beenie Jackson1Antony Joseph Raj2Murugan Sevanan3Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, Coimbatore 641114, IndiaDepartment of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, Coimbatore 641114, IndiaDepartment of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, Coimbatore 641114, IndiaDepartment of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, Coimbatore 641114, IndiaLaccases are the model enzymes for multicopper oxidases and participate in several applications such as bioremediation, biopulping, textile, and food industries. Laccase producing bacterium, Bacillus subtilis MTCC 2414, was subjected to optimization by conventional techniques and was partially purified using ammonium salt precipitation method. The agroresidue substrates used for higher yield of laccase were rice bran and wheat bran. Maximum production was achieved at temperature 30°C (270 ± 2.78 U/mL), pH 7.0 (345 ± 3.14 U/mL), and 96 h (267 ± 2.64 U/mL) of incubation. The carbon and nitrogen sources resulted in high enzyme yield at 3% sucrose (275 ± 3.11 U/mL) and 3% peptone (352.2 ± 4.32 U/mL) for rice bran and 3% sucrose (247.4 ± 3.51 U/mL) and 3% peptone (328 ± 3.33 U/mL) for wheat bran, respectively. The molecular weights of partially purified laccase were 52 kDa for rice bran and 55 kDa for wheat bran. The laccase exhibited optimal activity at 70°C (260.3 ± 6.15 U/mL), pH 9.0 (266 ± 4.02 U/mL), and metal ion CuSO4 (141.4 ± 6.64) was found to increase the production. This is the first report that delivers the higher yield of laccase produced from B. subtilis MTCC 2414 using agroresidues as a potential substrate.http://dx.doi.org/10.1155/2015/765190 |
| spellingShingle | Narayanan P. Muthukumarasamy Beenie Jackson Antony Joseph Raj Murugan Sevanan Production of Extracellular Laccase from Bacillus subtilis MTCC 2414 Using Agroresidues as a Potential Substrate Biochemistry Research International |
| title | Production of Extracellular Laccase from Bacillus subtilis MTCC 2414 Using Agroresidues as a Potential Substrate |
| title_full | Production of Extracellular Laccase from Bacillus subtilis MTCC 2414 Using Agroresidues as a Potential Substrate |
| title_fullStr | Production of Extracellular Laccase from Bacillus subtilis MTCC 2414 Using Agroresidues as a Potential Substrate |
| title_full_unstemmed | Production of Extracellular Laccase from Bacillus subtilis MTCC 2414 Using Agroresidues as a Potential Substrate |
| title_short | Production of Extracellular Laccase from Bacillus subtilis MTCC 2414 Using Agroresidues as a Potential Substrate |
| title_sort | production of extracellular laccase from bacillus subtilis mtcc 2414 using agroresidues as a potential substrate |
| url | http://dx.doi.org/10.1155/2015/765190 |
| work_keys_str_mv | AT narayananpmuthukumarasamy productionofextracellularlaccasefrombacillussubtilismtcc2414usingagroresiduesasapotentialsubstrate AT beeniejackson productionofextracellularlaccasefrombacillussubtilismtcc2414usingagroresiduesasapotentialsubstrate AT antonyjosephraj productionofextracellularlaccasefrombacillussubtilismtcc2414usingagroresiduesasapotentialsubstrate AT murugansevanan productionofextracellularlaccasefrombacillussubtilismtcc2414usingagroresiduesasapotentialsubstrate |