A Picrorhiza kurroa Derivative, Picroliv, Attenuates the Development of Dextran-Sulfate-Sodium-Induced Colitis in Mice
Background. Free radicals and proinflammatory cytokines have been shown to play a critical role in the pathogenesis of ulcerative colitis (UC). Picroliv, a Picrorhiza kurroa derivative, has been demonstrated to have antioxidant and anti-inflammatory effect. The purpose of the study was to investigat...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/751629 |
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| author | De-Kui Zhang Jian-Jie Yu Yu-Min Li Li-Na Wei Yi Yu Yan-Hu Feng Xiang Wang |
| author_facet | De-Kui Zhang Jian-Jie Yu Yu-Min Li Li-Na Wei Yi Yu Yan-Hu Feng Xiang Wang |
| author_sort | De-Kui Zhang |
| collection | DOAJ |
| description | Background. Free radicals and proinflammatory cytokines have been shown to play a critical role in the pathogenesis of ulcerative colitis (UC). Picroliv, a Picrorhiza kurroa derivative, has been demonstrated to have antioxidant and anti-inflammatory effect. The purpose of the study was to investigate the effects of picroliv on experimental model of UC in mice. Materials and Methods. Picroliv was administrated orally by gavage to mice with colitis induced by dextran sulfate sodium (DSS). Disease activity index (DAI), colon length, and histology score were observed. Myeloperoxidase (MPO) activity, and SOD, MDA concentrations were measured by enzyme-linked immunosorbent assay (ELISA) while the expression of cytokine mRNAs was studied by real-time-quantitative polymerase chain reaction and also ELISA. The expression of NF-κB p65 was observed by immunohistochemistry staining and western blotting. Results. A significant improvement was observed in DAI and histological score in mice treated with picroliv, and incerased MPO activity, MDA concentrations, and the expression of IL-1β, TNF-α, and NF-κB p65 in mice with DSS-induced colitis were significantly reduced while decreased SOD level increased following administration of picroliv. Conclusion. The administration of picroliv leads to an amelioration of DSS-induced colitis, suggesting administration of picroliv may provide a therapeutic approach for UC. |
| format | Article |
| id | doaj-art-a0f6f47cd47c4a1597837e7e6432390b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-a0f6f47cd47c4a1597837e7e6432390b2025-02-03T00:59:52ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/751629751629A Picrorhiza kurroa Derivative, Picroliv, Attenuates the Development of Dextran-Sulfate-Sodium-Induced Colitis in MiceDe-Kui Zhang0Jian-Jie Yu1Yu-Min Li2Li-Na Wei3Yi Yu4Yan-Hu Feng5Xiang Wang6Department of Gastroenterology, The Second Hospital, Lanzhou University, Gansu Province, Lanzhou 730000, ChinaDepartment of Gastroenterology, The Second Hospital, Lanzhou University, Gansu Province, Lanzhou 730000, ChinaKey Laboratory of Digestive System Tumors, Gansu Province, Lanzhou 730000, ChinaDepartment of Gastroenterology, The Second Hospital, Lanzhou University, Gansu Province, Lanzhou 730000, ChinaDepartment of Gastroenterology, The Second Hospital, Lanzhou University, Gansu Province, Lanzhou 730000, ChinaDepartment of Gastroenterology, The Second Hospital, Lanzhou University, Gansu Province, Lanzhou 730000, ChinaDepartment of Gastroenterology, The Second Hospital, Lanzhou University, Gansu Province, Lanzhou 730000, ChinaBackground. Free radicals and proinflammatory cytokines have been shown to play a critical role in the pathogenesis of ulcerative colitis (UC). Picroliv, a Picrorhiza kurroa derivative, has been demonstrated to have antioxidant and anti-inflammatory effect. The purpose of the study was to investigate the effects of picroliv on experimental model of UC in mice. Materials and Methods. Picroliv was administrated orally by gavage to mice with colitis induced by dextran sulfate sodium (DSS). Disease activity index (DAI), colon length, and histology score were observed. Myeloperoxidase (MPO) activity, and SOD, MDA concentrations were measured by enzyme-linked immunosorbent assay (ELISA) while the expression of cytokine mRNAs was studied by real-time-quantitative polymerase chain reaction and also ELISA. The expression of NF-κB p65 was observed by immunohistochemistry staining and western blotting. Results. A significant improvement was observed in DAI and histological score in mice treated with picroliv, and incerased MPO activity, MDA concentrations, and the expression of IL-1β, TNF-α, and NF-κB p65 in mice with DSS-induced colitis were significantly reduced while decreased SOD level increased following administration of picroliv. Conclusion. The administration of picroliv leads to an amelioration of DSS-induced colitis, suggesting administration of picroliv may provide a therapeutic approach for UC.http://dx.doi.org/10.1155/2012/751629 |
| spellingShingle | De-Kui Zhang Jian-Jie Yu Yu-Min Li Li-Na Wei Yi Yu Yan-Hu Feng Xiang Wang A Picrorhiza kurroa Derivative, Picroliv, Attenuates the Development of Dextran-Sulfate-Sodium-Induced Colitis in Mice Mediators of Inflammation |
| title | A Picrorhiza kurroa Derivative, Picroliv, Attenuates the Development of Dextran-Sulfate-Sodium-Induced Colitis in Mice |
| title_full | A Picrorhiza kurroa Derivative, Picroliv, Attenuates the Development of Dextran-Sulfate-Sodium-Induced Colitis in Mice |
| title_fullStr | A Picrorhiza kurroa Derivative, Picroliv, Attenuates the Development of Dextran-Sulfate-Sodium-Induced Colitis in Mice |
| title_full_unstemmed | A Picrorhiza kurroa Derivative, Picroliv, Attenuates the Development of Dextran-Sulfate-Sodium-Induced Colitis in Mice |
| title_short | A Picrorhiza kurroa Derivative, Picroliv, Attenuates the Development of Dextran-Sulfate-Sodium-Induced Colitis in Mice |
| title_sort | picrorhiza kurroa derivative picroliv attenuates the development of dextran sulfate sodium induced colitis in mice |
| url | http://dx.doi.org/10.1155/2012/751629 |
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