Plasmid Profiles and Prevalence of Intermediately Virulent Rhodococcus equi from Pigs in Nakhonpathom Province, Thailand: Identification of a New Variant of the 70-kb Virulence Plasmid, Type 18
The prevalence of intermediately virulent Rhodococcus equi isolates from pig submaxillary lymph nodes from four slaughterhouses in Nakhonpathom province, Thailand, was investigated. The isolates were tested for the presence of virulence plasmids and the 20-kDa virulence-associated protein antigen (V...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.4061/2010/491624 |
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| author | Chaithep Poolkhet Suksun Chumsing Worawidh Wajjwalku Chihiro Minato Yukiko Otsu Shinji Takai |
| author_facet | Chaithep Poolkhet Suksun Chumsing Worawidh Wajjwalku Chihiro Minato Yukiko Otsu Shinji Takai |
| author_sort | Chaithep Poolkhet |
| collection | DOAJ |
| description | The prevalence of intermediately virulent Rhodococcus equi isolates from pig submaxillary lymph nodes from four slaughterhouses in Nakhonpathom province, Thailand, was investigated. The isolates were tested for the presence of virulence plasmids and the 20-kDa virulence-associated protein antigen (VapB) gene by PCR. Of the 734 submaxillary lymph nodes tested, 19 (2.6%) produced positive cultures of R. equi. All 19 isolates were positive for the VapB gene and contained virulence plasmids that were identified as type 1 (six isolates), type 6 (two isolates), type 7 (one isolate), type 16 (two isolates), and a new variant (eight isolates). Based on the restriction digestion patterns of the plasmid DNAs, we tentatively designated the variant as type 18. Investigation of the prevalence and plasmid profiles of VapB-positive R. equi in pigs should be extended throughout Thailand to evaluate potential sources of zoonotic infections. |
| format | Article |
| id | doaj-art-a0f58f7843cf45cd9d79bf434204bae7 |
| institution | Kabale University |
| issn | 2042-0048 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-a0f58f7843cf45cd9d79bf434204bae72025-02-03T01:26:19ZengWileyVeterinary Medicine International2042-00482010-01-01201010.4061/2010/491624491624Plasmid Profiles and Prevalence of Intermediately Virulent Rhodococcus equi from Pigs in Nakhonpathom Province, Thailand: Identification of a New Variant of the 70-kb Virulence Plasmid, Type 18Chaithep Poolkhet0Suksun Chumsing1Worawidh Wajjwalku2Chihiro Minato3Yukiko Otsu4Shinji Takai5Faculty of Veterinary Medicine, Kasetsart University, Nakhonpathom 73140, ThailandFaculty of Veterinary Medicine, Kasetsart University, Nakhonpathom 73140, ThailandFaculty of Veterinary Medicine, Kasetsart University, Nakhonpathom 73140, ThailandSchool of Veterinary Medicine and Animal Science, Kitasato University, Aomori 034-8628, JapanSchool of Veterinary Medicine and Animal Science, Kitasato University, Aomori 034-8628, JapanSchool of Veterinary Medicine and Animal Science, Kitasato University, Aomori 034-8628, JapanThe prevalence of intermediately virulent Rhodococcus equi isolates from pig submaxillary lymph nodes from four slaughterhouses in Nakhonpathom province, Thailand, was investigated. The isolates were tested for the presence of virulence plasmids and the 20-kDa virulence-associated protein antigen (VapB) gene by PCR. Of the 734 submaxillary lymph nodes tested, 19 (2.6%) produced positive cultures of R. equi. All 19 isolates were positive for the VapB gene and contained virulence plasmids that were identified as type 1 (six isolates), type 6 (two isolates), type 7 (one isolate), type 16 (two isolates), and a new variant (eight isolates). Based on the restriction digestion patterns of the plasmid DNAs, we tentatively designated the variant as type 18. Investigation of the prevalence and plasmid profiles of VapB-positive R. equi in pigs should be extended throughout Thailand to evaluate potential sources of zoonotic infections.http://dx.doi.org/10.4061/2010/491624 |
| spellingShingle | Chaithep Poolkhet Suksun Chumsing Worawidh Wajjwalku Chihiro Minato Yukiko Otsu Shinji Takai Plasmid Profiles and Prevalence of Intermediately Virulent Rhodococcus equi from Pigs in Nakhonpathom Province, Thailand: Identification of a New Variant of the 70-kb Virulence Plasmid, Type 18 Veterinary Medicine International |
| title | Plasmid Profiles and Prevalence of Intermediately Virulent Rhodococcus equi from Pigs in Nakhonpathom Province, Thailand: Identification of a New Variant of the 70-kb Virulence Plasmid, Type 18 |
| title_full | Plasmid Profiles and Prevalence of Intermediately Virulent Rhodococcus equi from Pigs in Nakhonpathom Province, Thailand: Identification of a New Variant of the 70-kb Virulence Plasmid, Type 18 |
| title_fullStr | Plasmid Profiles and Prevalence of Intermediately Virulent Rhodococcus equi from Pigs in Nakhonpathom Province, Thailand: Identification of a New Variant of the 70-kb Virulence Plasmid, Type 18 |
| title_full_unstemmed | Plasmid Profiles and Prevalence of Intermediately Virulent Rhodococcus equi from Pigs in Nakhonpathom Province, Thailand: Identification of a New Variant of the 70-kb Virulence Plasmid, Type 18 |
| title_short | Plasmid Profiles and Prevalence of Intermediately Virulent Rhodococcus equi from Pigs in Nakhonpathom Province, Thailand: Identification of a New Variant of the 70-kb Virulence Plasmid, Type 18 |
| title_sort | plasmid profiles and prevalence of intermediately virulent rhodococcus equi from pigs in nakhonpathom province thailand identification of a new variant of the 70 kb virulence plasmid type 18 |
| url | http://dx.doi.org/10.4061/2010/491624 |
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