Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using Immunoinformatics
Pseudomonas aeruginosa is a common pathogen that is responsible for serious hospital-acquired infections, ventilator-associated pneumonia, and various sepsis syndromes. Also, it is a multidrug-resistant pathogen recognized for its ubiquity and its intrinsically advanced antibiotic-resistant mechanis...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/9475058 |
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| author | Mustafa Elhag Ruaa Mohamed Alaagib Nagla Mohamed Ahmed Mustafa Abubaker Esraa Musa Haroun Sahar Obi Abd Albagi Mohammed A. Hassan |
| author_facet | Mustafa Elhag Ruaa Mohamed Alaagib Nagla Mohamed Ahmed Mustafa Abubaker Esraa Musa Haroun Sahar Obi Abd Albagi Mohammed A. Hassan |
| author_sort | Mustafa Elhag |
| collection | DOAJ |
| description | Pseudomonas aeruginosa is a common pathogen that is responsible for serious hospital-acquired infections, ventilator-associated pneumonia, and various sepsis syndromes. Also, it is a multidrug-resistant pathogen recognized for its ubiquity and its intrinsically advanced antibiotic-resistant mechanisms. It usually affects immunocompromised individuals but can also infect immunocompetent individuals. There is no vaccine against it available till now. This study predicts an effective epitope-based vaccine against fructose bisphosphate aldolase (FBA) of Pseudomonas aeruginosa using immunoinformatics tools. The protein sequences were obtained from NCBI, and prediction tests were undertaken to analyze possible epitopes for B and T cells. Three B cell epitopes passed the antigenicity, accessibility, and hydrophilicity tests. Six MHC I epitopes were found to be promising, while four MHC II epitopes were found promising from the result set. Nineteen epitopes were shared between MHC I and II results. For the population coverage, the epitopes covered 95.62% worldwide excluding certain MHC II alleles. We recommend in vivo and in vitro studies to prove its effectiveness. |
| format | Article |
| id | doaj-art-a0de6250d4e043d7ac97adb24b8954a7 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-a0de6250d4e043d7ac97adb24b8954a72025-02-03T06:05:38ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/94750589475058Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using ImmunoinformaticsMustafa Elhag0Ruaa Mohamed Alaagib1Nagla Mohamed Ahmed2Mustafa Abubaker3Esraa Musa Haroun4Sahar Obi Abd Albagi5Mohammed A. Hassan6Faculty of Medicine, University of Seychelles-American Institute of Medicine, SeychellesDepartment of Pharmacies, National Medical Supplies Fund, SudanFaculty of Medical Laboratories Sciences, Al-Neelain University, SudanFaculty of Medical Laboratory Sciences, Sudan University of Science and Technology, SudanFaculty of Medical Pharmacology, Ahfad University for Women, SudanFaculty of Medical Laboratories Sciences, Al-Neelain University, SudanDepartment of Bioinformatics, DETAGEN Genetics Diagnostic Center, Kayseri, TurkeyPseudomonas aeruginosa is a common pathogen that is responsible for serious hospital-acquired infections, ventilator-associated pneumonia, and various sepsis syndromes. Also, it is a multidrug-resistant pathogen recognized for its ubiquity and its intrinsically advanced antibiotic-resistant mechanisms. It usually affects immunocompromised individuals but can also infect immunocompetent individuals. There is no vaccine against it available till now. This study predicts an effective epitope-based vaccine against fructose bisphosphate aldolase (FBA) of Pseudomonas aeruginosa using immunoinformatics tools. The protein sequences were obtained from NCBI, and prediction tests were undertaken to analyze possible epitopes for B and T cells. Three B cell epitopes passed the antigenicity, accessibility, and hydrophilicity tests. Six MHC I epitopes were found to be promising, while four MHC II epitopes were found promising from the result set. Nineteen epitopes were shared between MHC I and II results. For the population coverage, the epitopes covered 95.62% worldwide excluding certain MHC II alleles. We recommend in vivo and in vitro studies to prove its effectiveness.http://dx.doi.org/10.1155/2020/9475058 |
| spellingShingle | Mustafa Elhag Ruaa Mohamed Alaagib Nagla Mohamed Ahmed Mustafa Abubaker Esraa Musa Haroun Sahar Obi Abd Albagi Mohammed A. Hassan Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using Immunoinformatics Journal of Immunology Research |
| title | Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using Immunoinformatics |
| title_full | Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using Immunoinformatics |
| title_fullStr | Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using Immunoinformatics |
| title_full_unstemmed | Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using Immunoinformatics |
| title_short | Design of Epitope-Based Peptide Vaccine against Pseudomonas aeruginosa Fructose Bisphosphate Aldolase Protein Using Immunoinformatics |
| title_sort | design of epitope based peptide vaccine against pseudomonas aeruginosa fructose bisphosphate aldolase protein using immunoinformatics |
| url | http://dx.doi.org/10.1155/2020/9475058 |
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