70% ethanol preserves mycobacterial RNA from cultures more efficiently than GTC-TCEP

70% ethanol preserves mycobacterial RNA from cultures more efficiently than GTC-TCEP

Abstract RNA-based assays hold great potential for assessing viability molecularly in slow- or non-growing mycobacteria, and RNAseq has evolved into a powerful tool in infectious disease research. Such applications require efficient RNA preservation for optimal results. The performance of 70% ethano...

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Main Authors: Lena Krausser, Magalie Van Dyck-Lippens, Ramata Balde, Rabab Reenaers, Reem Al Mubarak, Leen Rigouts, Nicholas D. Walter, Martin I. Voskuil, Annelies Van Rie, Bouke C. de Jong, Sofie M. Braet
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
Subjects:
RNA
Preservation
Ethanol
GTC
Mycobacterium tuberculosis
Mycobacterium leprae
Online Access:https://doi.org/10.1038/s41598-025-93699-7
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https://doi.org/10.1038/s41598-025-93699-7

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