Evolutionary rescue of spherical mreB deletion mutants of the rod-shape bacterium Pseudomonas fluorescens SBW25

Evolutionary rescue of spherical mreB deletion mutants of the rod-shape bacterium Pseudomonas fluorescens SBW25

Maintenance of rod-shape in bacterial cells depends on the actin-like protein MreB. Deletion of mreB from Pseudomonas fluorescens SBW25 results in viable spherical cells of variable volume and reduced fitness. Using a combination of time-resolved microscopy and biochemical assay of peptidoglycan syn...

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Bibliographic Details
Main Authors: Paul Richard J Yulo, Nicolas Desprat, Monica L Gerth, Barbara Ritzl-Rinkenberger, Andrew D Farr, Yunhao Liu, Xue-Xian Zhang, Michael Miller, Felipe Cava, Paul B Rainey, Heather L Hendrickson
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2025-03-01
Series:eLife
Subjects:
Pseudomonas fluorescens SBW25
compensatory evolution
cell shape
Online Access:https://elifesciences.org/articles/98218
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https://elifesciences.org/articles/98218

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