Optical clearing of apple tissues for in vivo imaging of the pathogenic behavior of the fungus Botryosphaeria dothidea on host surfaces

Abstract Optical clearing of apple tissues was performed to observe the pre-penetration behavior of Botryosphaeria dothidea. Mature red fruits and two-year-old twigs were artificially inoculated with the fungal conidia. Fruit epidermis and twig cork tissues were excised and immersed overnight in an...

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Bibliographic Details
Main Authors: Ki Woo Kim, Eun Woo Park
Format: Article
Language:English
Published: SpringerOpen 2025-05-01
Series:Applied Microscopy
Subjects:
Online Access:https://doi.org/10.1186/s42649-025-00110-w
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