Study of african swine fevervirus reproduction in porcine primary bone marrow cell culture before and after cryopreservation
This paper presents data on the preparation of cells from porcine bone marrow, spleen, lymph nodes, kidneys and testicles used for African swine fever virus reproduction. Viability of cells after cryopreservation was studied only in porcine bone marrow cell culture. The cell culture was frozen and s...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2018-04-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/226 |
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| author | I. Yu. Zhukov I. V. Shevchenko N. N. Vlasova A. A. Varentsova B. L. Manin O. S. Puzankova V. L. Gavrilova A. S. Igolkin |
| author_facet | I. Yu. Zhukov I. V. Shevchenko N. N. Vlasova A. A. Varentsova B. L. Manin O. S. Puzankova V. L. Gavrilova A. S. Igolkin |
| author_sort | I. Yu. Zhukov |
| collection | DOAJ |
| description | This paper presents data on the preparation of cells from porcine bone marrow, spleen, lymph nodes, kidneys and testicles used for African swine fever virus reproduction. Viability of cells after cryopreservation was studied only in porcine bone marrow cell culture. The cell culture was frozen and stored at -70, -150 and -196°C. 10% dimethyl sulfide supplemented with 10% of fetal bovine serum was used as a cryoprotectant. When the cells were thawed and the cryoprotectant was removed 10 lU/ml of heparin sodium was added to prevent cell aggregation. ASFV culturing in porcine bone marrow cell culture stored at -150 and -196°C during 5-6 months demonstrated the highest haemadsorption titers: 5,76±0,25 Ig HAdU50/0,15 cm3 and (4,66-4,87)±0,25 Ig HAdU50/0,15 cm3, respectively. Porcine bone marrow cells remained viable only for four months of storage at -70°C and were able to maintain the virus reproduction at the level of (3,2-3,86)±0,25 Ig HAdU50/0,15 cm3 when thawed. |
| format | Article |
| id | doaj-art-9fcf59868092466aa4d3991b4b083dbb |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2018-04-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-9fcf59868092466aa4d3991b4b083dbb2025-02-06T09:52:05ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-04-0101715226Study of african swine fevervirus reproduction in porcine primary bone marrow cell culture before and after cryopreservationI. Yu. Zhukov0I. V. Shevchenko1N. N. Vlasova2A. A. Varentsova3B. L. Manin4O. S. Puzankova5V. L. Gavrilova6A. S. Igolkin7FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirThis paper presents data on the preparation of cells from porcine bone marrow, spleen, lymph nodes, kidneys and testicles used for African swine fever virus reproduction. Viability of cells after cryopreservation was studied only in porcine bone marrow cell culture. The cell culture was frozen and stored at -70, -150 and -196°C. 10% dimethyl sulfide supplemented with 10% of fetal bovine serum was used as a cryoprotectant. When the cells were thawed and the cryoprotectant was removed 10 lU/ml of heparin sodium was added to prevent cell aggregation. ASFV culturing in porcine bone marrow cell culture stored at -150 and -196°C during 5-6 months demonstrated the highest haemadsorption titers: 5,76±0,25 Ig HAdU50/0,15 cm3 and (4,66-4,87)±0,25 Ig HAdU50/0,15 cm3, respectively. Porcine bone marrow cells remained viable only for four months of storage at -70°C and were able to maintain the virus reproduction at the level of (3,2-3,86)±0,25 Ig HAdU50/0,15 cm3 when thawed.https://veterinary.arriah.ru/jour/article/view/226вирус африканской чумы свинейкультура клеток костного мозга свинейгемадсорбирующий титр вирусаafrican swine fever virusporcine bone marrow cell culturevirus hemadsorption titer |
| spellingShingle | I. Yu. Zhukov I. V. Shevchenko N. N. Vlasova A. A. Varentsova B. L. Manin O. S. Puzankova V. L. Gavrilova A. S. Igolkin Study of african swine fevervirus reproduction in porcine primary bone marrow cell culture before and after cryopreservation Ветеринария сегодня вирус африканской чумы свиней культура клеток костного мозга свиней гемадсорбирующий титр вируса african swine fever virus porcine bone marrow cell culture virus hemadsorption titer |
| title | Study of african swine fevervirus reproduction in porcine primary bone marrow cell culture before and after cryopreservation |
| title_full | Study of african swine fevervirus reproduction in porcine primary bone marrow cell culture before and after cryopreservation |
| title_fullStr | Study of african swine fevervirus reproduction in porcine primary bone marrow cell culture before and after cryopreservation |
| title_full_unstemmed | Study of african swine fevervirus reproduction in porcine primary bone marrow cell culture before and after cryopreservation |
| title_short | Study of african swine fevervirus reproduction in porcine primary bone marrow cell culture before and after cryopreservation |
| title_sort | study of african swine fevervirus reproduction in porcine primary bone marrow cell culture before and after cryopreservation |
| topic | вирус африканской чумы свиней культура клеток костного мозга свиней гемадсорбирующий титр вируса african swine fever virus porcine bone marrow cell culture virus hemadsorption titer |
| url | https://veterinary.arriah.ru/jour/article/view/226 |
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