Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing
Due to the call for fast KRAS mutation status analysis for treatment of patients with monoclonal antibodies for metastatic colorectal cancer, sensitive, economic, and easily feasible methods are required. Under this aspect, the sensitivity and specificity of the SNaPshot analysis in comparison to th...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
|
| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2010/789363 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548136388657152 |
|---|---|
| author | Rica Zinsky Servet Bölükbas Holger Bartsch Joachim Schirren Annette Fisseler-Eckhoff |
| author_facet | Rica Zinsky Servet Bölükbas Holger Bartsch Joachim Schirren Annette Fisseler-Eckhoff |
| author_sort | Rica Zinsky |
| collection | DOAJ |
| description | Due to the call for fast KRAS mutation status analysis for treatment of patients with monoclonal antibodies for metastatic colorectal cancer, sensitive, economic, and easily feasible methods are required. Under this aspect, the sensitivity and specificity of the SNaPshot analysis in comparison to the commonly used DNA sequencing was checked. We examined KRAS mutations in exon 2 codons 12 and 13 with DNA sequencing and SNaPshot analysis in 100 formalin-fixed paraffin-embedded tumor tissue samples of pancreatic carcinoma, colorectal carcinoma, and nonsmall cell lung cancer specimens of the primary tumor or metastases. 40% of these samples demonstrated mutated KRAS genes using sequencing and SNaPshot-analysis; additional five samples (45/100) were identified only with the SNaPshot. KRAS mutation detection is feasible with the reliable SNaPshot analysis method. The more frequent mutation detection by the SNaPshot analysis shows that this method has a high probability of accuracy in the detection of KRAS mutations compared to sequencing. |
| format | Article |
| id | doaj-art-9fcd561ce8db4e489bd1b706bb8f69c5 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-9fcd561ce8db4e489bd1b706bb8f69c52025-02-03T06:42:08ZengWileyGastroenterology Research and Practice1687-61211687-630X2010-01-01201010.1155/2010/789363789363Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA SequencingRica Zinsky0Servet Bölükbas1Holger Bartsch2Joachim Schirren3Annette Fisseler-Eckhoff4Institute of Pathology and Cytology, HSK Wiesbaden, Academic Hospital of University of Mainz, Ludwig-Erhard-Strasse 100, 65199 Wiesbaden, GermanyDepartment of Thoracic Surgery, HSK Wiesbaden, Academic Hospital of University of Mainz, Ludwig-Erhard-Strasse 100, 65199 Wiesbaden, GermanyInstitute of Pathology and Cytology, HSK Wiesbaden, Academic Hospital of University of Mainz, Ludwig-Erhard-Strasse 100, 65199 Wiesbaden, GermanyDepartment of Thoracic Surgery, HSK Wiesbaden, Academic Hospital of University of Mainz, Ludwig-Erhard-Strasse 100, 65199 Wiesbaden, GermanyInstitute of Pathology and Cytology, HSK Wiesbaden, Academic Hospital of University of Mainz, Ludwig-Erhard-Strasse 100, 65199 Wiesbaden, GermanyDue to the call for fast KRAS mutation status analysis for treatment of patients with monoclonal antibodies for metastatic colorectal cancer, sensitive, economic, and easily feasible methods are required. Under this aspect, the sensitivity and specificity of the SNaPshot analysis in comparison to the commonly used DNA sequencing was checked. We examined KRAS mutations in exon 2 codons 12 and 13 with DNA sequencing and SNaPshot analysis in 100 formalin-fixed paraffin-embedded tumor tissue samples of pancreatic carcinoma, colorectal carcinoma, and nonsmall cell lung cancer specimens of the primary tumor or metastases. 40% of these samples demonstrated mutated KRAS genes using sequencing and SNaPshot-analysis; additional five samples (45/100) were identified only with the SNaPshot. KRAS mutation detection is feasible with the reliable SNaPshot analysis method. The more frequent mutation detection by the SNaPshot analysis shows that this method has a high probability of accuracy in the detection of KRAS mutations compared to sequencing.http://dx.doi.org/10.1155/2010/789363 |
| spellingShingle | Rica Zinsky Servet Bölükbas Holger Bartsch Joachim Schirren Annette Fisseler-Eckhoff Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing Gastroenterology Research and Practice |
| title | Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing |
| title_full | Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing |
| title_fullStr | Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing |
| title_full_unstemmed | Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing |
| title_short | Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing |
| title_sort | analysis of kras mutations of exon 2 codons 12 and 13 by snapshot analysis in comparison to common dna sequencing |
| url | http://dx.doi.org/10.1155/2010/789363 |
| work_keys_str_mv | AT ricazinsky analysisofkrasmutationsofexon2codons12and13bysnapshotanalysisincomparisontocommondnasequencing AT servetbolukbas analysisofkrasmutationsofexon2codons12and13bysnapshotanalysisincomparisontocommondnasequencing AT holgerbartsch analysisofkrasmutationsofexon2codons12and13bysnapshotanalysisincomparisontocommondnasequencing AT joachimschirren analysisofkrasmutationsofexon2codons12and13bysnapshotanalysisincomparisontocommondnasequencing AT annettefisselereckhoff analysisofkrasmutationsofexon2codons12and13bysnapshotanalysisincomparisontocommondnasequencing |