Production of β-Glucosidase from a Newly Isolated Aspergillus Species Using Response Surface Methodology
A newly isolated fungus Aspergillus niger SOI017 was shown to be a good producer of β-glucosidase from all isolated fungal strains. Fermentation condition (pH, cellobiose concentration, yeast extract concentration, and ammonium sulfate concentration) was optimized for producing the enzyme in shake f...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2011/949252 |
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| _version_ | 1832567814082265088 |
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| author | Pilanee Vaithanomsat Molnapat Songpim Taweesiri Malapant Akihiko Kosugi Warunee Thanapase Yutaka Mori |
| author_facet | Pilanee Vaithanomsat Molnapat Songpim Taweesiri Malapant Akihiko Kosugi Warunee Thanapase Yutaka Mori |
| author_sort | Pilanee Vaithanomsat |
| collection | DOAJ |
| description | A newly isolated fungus Aspergillus niger SOI017 was shown to be a good producer of β-glucosidase from all isolated fungal strains. Fermentation condition (pH, cellobiose concentration, yeast extract concentration, and ammonium sulfate concentration) was optimized for producing the enzyme in shake flask cultures. Response surface methodology was used to investigate the effects of 4 fermentation parameters (yeast extract concentration, cellobiose concentration, ammonium sulfate concentration, and pH) on β-glucosidase enzyme production. Production of β-glucosidase was most sensitive to the culture medium, especially the nitrogen source yeast extract. The optimized medium for producing maximum β-glucosidase specific activity consisted of 0.275% yeast extract, 1.125% cellobiose, and 2.6% ammonium sulfate at a pH value of 3. |
| format | Article |
| id | doaj-art-9fc7e48e0002429bacc99c95a1d48740 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-9fc7e48e0002429bacc99c95a1d487402025-02-03T01:00:29ZengWileyInternational Journal of Microbiology1687-918X1687-91982011-01-01201110.1155/2011/949252949252Production of β-Glucosidase from a Newly Isolated Aspergillus Species Using Response Surface MethodologyPilanee Vaithanomsat0Molnapat Songpim1Taweesiri Malapant2Akihiko Kosugi3Warunee Thanapase4Yutaka Mori5Enzyme Technology and Waste Management Research Unit, Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, 50, Chatuchak, Bangkok 10900, ThailandEnzyme Technology and Waste Management Research Unit, Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, 50, Chatuchak, Bangkok 10900, ThailandEnzyme Technology and Waste Management Research Unit, Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, 50, Chatuchak, Bangkok 10900, ThailandPost-Harvest Science and Technology Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, JapanEnzyme Technology and Waste Management Research Unit, Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, 50, Chatuchak, Bangkok 10900, ThailandPost-Harvest Science and Technology Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1, Ohwashi, Tsukuba, Ibaraki 305-8686, JapanA newly isolated fungus Aspergillus niger SOI017 was shown to be a good producer of β-glucosidase from all isolated fungal strains. Fermentation condition (pH, cellobiose concentration, yeast extract concentration, and ammonium sulfate concentration) was optimized for producing the enzyme in shake flask cultures. Response surface methodology was used to investigate the effects of 4 fermentation parameters (yeast extract concentration, cellobiose concentration, ammonium sulfate concentration, and pH) on β-glucosidase enzyme production. Production of β-glucosidase was most sensitive to the culture medium, especially the nitrogen source yeast extract. The optimized medium for producing maximum β-glucosidase specific activity consisted of 0.275% yeast extract, 1.125% cellobiose, and 2.6% ammonium sulfate at a pH value of 3.http://dx.doi.org/10.1155/2011/949252 |
| spellingShingle | Pilanee Vaithanomsat Molnapat Songpim Taweesiri Malapant Akihiko Kosugi Warunee Thanapase Yutaka Mori Production of β-Glucosidase from a Newly Isolated Aspergillus Species Using Response Surface Methodology International Journal of Microbiology |
| title | Production of β-Glucosidase from a Newly Isolated Aspergillus Species Using Response Surface Methodology |
| title_full | Production of β-Glucosidase from a Newly Isolated Aspergillus Species Using Response Surface Methodology |
| title_fullStr | Production of β-Glucosidase from a Newly Isolated Aspergillus Species Using Response Surface Methodology |
| title_full_unstemmed | Production of β-Glucosidase from a Newly Isolated Aspergillus Species Using Response Surface Methodology |
| title_short | Production of β-Glucosidase from a Newly Isolated Aspergillus Species Using Response Surface Methodology |
| title_sort | production of β glucosidase from a newly isolated aspergillus species using response surface methodology |
| url | http://dx.doi.org/10.1155/2011/949252 |
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