Epithelium-dependent effect of L-glutamate on airways: involvement of prostaglandins
We investigated the effect of the excitatory amino acid (EAA) receptor agonists L-glutamate, N-methyl-D-aspartate (NMDA), (RS)-a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainic acid on KCl-induced contractions of rabbit tracheal smooth muscle, as well as the role of epithelium...
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| Format: | Article |
| Language: | English |
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Wiley
2002-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629350210312 |
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| author | Apostolia A. Hatziefthimiou Konstantinos I. Gourgoulianis Paschalis-Adam Molyvdas |
| author_facet | Apostolia A. Hatziefthimiou Konstantinos I. Gourgoulianis Paschalis-Adam Molyvdas |
| author_sort | Apostolia A. Hatziefthimiou |
| collection | DOAJ |
| description | We investigated the effect of the excitatory amino acid (EAA) receptor agonists L-glutamate, N-methyl-D-aspartate (NMDA), (RS)-a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainic acid on KCl-induced contractions of rabbit tracheal smooth muscle, as well as the role of epithelium and endogenously produced nitric oxide and prostaglandins on these responses. L-Glutamate decreased KCl-induced contractions up to 30%. This effect was attenuated by epithelium removal, tetrodotoxin, methylene blue and indomethacin but not by NG-nitro-L-arginine methyl ester. While NMDA, AMPA and kainic acid had no effect, the combination of NMDA + kainic acid decreased KCl-induced contractions. These results suggest that, in rabbit trachea, L-glutamate has, at least in part, an epithelium-dependent effect mediated via prostaglandin formation and that the EAA receptors involved are nonclassical. |
| format | Article |
| id | doaj-art-9f0673b87fd04ab896b6a24c8480ce5f |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-9f0673b87fd04ab896b6a24c8480ce5f2025-02-03T01:10:24ZengWileyMediators of Inflammation0962-93511466-18612002-01-01111333810.1080/09629350210312Epithelium-dependent effect of L-glutamate on airways: involvement of prostaglandinsApostolia A. Hatziefthimiou0Konstantinos I. Gourgoulianis1Paschalis-Adam Molyvdas2Department of Physiology, Medical School, University of Thessaly, Papakiriazi 22, Larissa 412 22, GreeceDepartment of Physiology, Medical School, University of Thessaly, Papakiriazi 22, Larissa 412 22, GreeceDepartment of Physiology, Medical School, University of Thessaly, Papakiriazi 22, Larissa 412 22, GreeceWe investigated the effect of the excitatory amino acid (EAA) receptor agonists L-glutamate, N-methyl-D-aspartate (NMDA), (RS)-a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainic acid on KCl-induced contractions of rabbit tracheal smooth muscle, as well as the role of epithelium and endogenously produced nitric oxide and prostaglandins on these responses. L-Glutamate decreased KCl-induced contractions up to 30%. This effect was attenuated by epithelium removal, tetrodotoxin, methylene blue and indomethacin but not by NG-nitro-L-arginine methyl ester. While NMDA, AMPA and kainic acid had no effect, the combination of NMDA + kainic acid decreased KCl-induced contractions. These results suggest that, in rabbit trachea, L-glutamate has, at least in part, an epithelium-dependent effect mediated via prostaglandin formation and that the EAA receptors involved are nonclassical.http://dx.doi.org/10.1080/09629350210312 |
| spellingShingle | Apostolia A. Hatziefthimiou Konstantinos I. Gourgoulianis Paschalis-Adam Molyvdas Epithelium-dependent effect of L-glutamate on airways: involvement of prostaglandins Mediators of Inflammation |
| title | Epithelium-dependent effect of L-glutamate on airways: involvement of prostaglandins |
| title_full | Epithelium-dependent effect of L-glutamate on airways: involvement of prostaglandins |
| title_fullStr | Epithelium-dependent effect of L-glutamate on airways: involvement of prostaglandins |
| title_full_unstemmed | Epithelium-dependent effect of L-glutamate on airways: involvement of prostaglandins |
| title_short | Epithelium-dependent effect of L-glutamate on airways: involvement of prostaglandins |
| title_sort | epithelium dependent effect of l glutamate on airways involvement of prostaglandins |
| url | http://dx.doi.org/10.1080/09629350210312 |
| work_keys_str_mv | AT apostoliaahatziefthimiou epitheliumdependenteffectoflglutamateonairwaysinvolvementofprostaglandins AT konstantinosigourgoulianis epitheliumdependenteffectoflglutamateonairwaysinvolvementofprostaglandins AT paschalisadammolyvdas epitheliumdependenteffectoflglutamateonairwaysinvolvementofprostaglandins |