Evaluation of Hepatoprotective Potential of Polyherbal Preparations in CCl4-Induced Hepatotoxicity in Mice
Background. Polyherbal formulations (PLFs) have been widely used for liver protection, treatment for hepatic dysfunction, and regeneration. They can also enhance appetite and protect the gastrointestinal tract from injury. In spite of the prevalent use, there is a need of scientific evidence on thei...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Advances in Pharmacological and Pharmaceutical Sciences |
| Online Access: | http://dx.doi.org/10.1155/2022/3169500 |
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| author | Rayhana Begum Sonia Akther Papia Mst Marium Begum Hongbin Wang Rubaba Karim Rebeka Sultana Priyanka Rani Das Taslima Begum Md. Ragibul Islam Nargis Manwar Md. Sohanur Rahman |
| author_facet | Rayhana Begum Sonia Akther Papia Mst Marium Begum Hongbin Wang Rubaba Karim Rebeka Sultana Priyanka Rani Das Taslima Begum Md. Ragibul Islam Nargis Manwar Md. Sohanur Rahman |
| author_sort | Rayhana Begum |
| collection | DOAJ |
| description | Background. Polyherbal formulations (PLFs) have been widely used for liver protection, treatment for hepatic dysfunction, and regeneration. They can also enhance appetite and protect the gastrointestinal tract from injury. In spite of the prevalent use, there is a need of scientific evidence on their effectiveness and safety. The objective of the present study was to assess the hepatoprotective effect of polyherbal formulations (commercially available in Bangladesh namely Heptaliv, Holyliv, Icturn, and J-deenar) in CCl4-induced hepatotoxicity in mice. Methods. In this study, Swiss albino mice were treated for 7 days with distilled water or PLFs (2.6 and 5.2 ml/kg body weight/day, per os.) followed by single subcutaneous injection of CCl4 (1 ml/kg body weight, diluted with olive oil in 1 : 1 ratio) on day 8. Twenty-four hours after CCl4 administration, the mice were monitored for the effects of PLFs on liver morphology, biochemical parameters including serum aspartate transaminase (AST), serum alanine transaminase (ALT), alkaline phosphatase (ALP), and total bilirubin. Phenobarbitone-induced sleeping time and histopathology changes in liver tissues were also monitored. Results. CCl4 administration caused significant hepatotoxicity as evidenced by marked elevation in AST, ALT, ALP, and total bilirubin. Phenobarbitone-induced sleeping time and infiltration of inflammatory cells and centrizonal necrosis on histological examination of liver demonstrated hepatic injury after CCl4 administration. However, the administration of Icturn and J-deenar polyherbal formulations at the higher dose significantly decreased the levels of AST, ALT, ALP, and total bilirubin. Moreover, pentobarbitone-induced sleeping time and histopathological analysis also revealed significant improvement as result of treatment with formulations Icturn and J-deenar. Conclusion. Our results confirmed that polyherbal formulations (Icturn and J-deenar) can significantly prevent CCl4-induced hepatotoxicity in mice, demonstrating their protective effect for liver. |
| format | Article |
| id | doaj-art-9de35313c604449f92a7d6b7fe5e6a56 |
| institution | Kabale University |
| issn | 2633-4690 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological and Pharmaceutical Sciences |
| spelling | doaj-art-9de35313c604449f92a7d6b7fe5e6a562025-02-03T06:06:48ZengWileyAdvances in Pharmacological and Pharmaceutical Sciences2633-46902022-01-01202210.1155/2022/3169500Evaluation of Hepatoprotective Potential of Polyherbal Preparations in CCl4-Induced Hepatotoxicity in MiceRayhana Begum0Sonia Akther Papia1Mst Marium Begum2Hongbin Wang3Rubaba Karim4Rebeka Sultana5Priyanka Rani Das6Taslima Begum7Md. Ragibul Islam8Nargis Manwar9Md. Sohanur Rahman10Department of PharmacyDepartment of PharmacyDepartment of PharmacyDepartment of Pharmaceutical and Biomedical SciencesDepartment of PharmacySchool of Molecular Medical SciencesDepartment of PharmacyDepartment of PharmacyDepartment of PharmacyDepartment of PharmacyDepartment of Biochemistry and Molecular BiologyBackground. Polyherbal formulations (PLFs) have been widely used for liver protection, treatment for hepatic dysfunction, and regeneration. They can also enhance appetite and protect the gastrointestinal tract from injury. In spite of the prevalent use, there is a need of scientific evidence on their effectiveness and safety. The objective of the present study was to assess the hepatoprotective effect of polyherbal formulations (commercially available in Bangladesh namely Heptaliv, Holyliv, Icturn, and J-deenar) in CCl4-induced hepatotoxicity in mice. Methods. In this study, Swiss albino mice were treated for 7 days with distilled water or PLFs (2.6 and 5.2 ml/kg body weight/day, per os.) followed by single subcutaneous injection of CCl4 (1 ml/kg body weight, diluted with olive oil in 1 : 1 ratio) on day 8. Twenty-four hours after CCl4 administration, the mice were monitored for the effects of PLFs on liver morphology, biochemical parameters including serum aspartate transaminase (AST), serum alanine transaminase (ALT), alkaline phosphatase (ALP), and total bilirubin. Phenobarbitone-induced sleeping time and histopathology changes in liver tissues were also monitored. Results. CCl4 administration caused significant hepatotoxicity as evidenced by marked elevation in AST, ALT, ALP, and total bilirubin. Phenobarbitone-induced sleeping time and infiltration of inflammatory cells and centrizonal necrosis on histological examination of liver demonstrated hepatic injury after CCl4 administration. However, the administration of Icturn and J-deenar polyherbal formulations at the higher dose significantly decreased the levels of AST, ALT, ALP, and total bilirubin. Moreover, pentobarbitone-induced sleeping time and histopathological analysis also revealed significant improvement as result of treatment with formulations Icturn and J-deenar. Conclusion. Our results confirmed that polyherbal formulations (Icturn and J-deenar) can significantly prevent CCl4-induced hepatotoxicity in mice, demonstrating their protective effect for liver.http://dx.doi.org/10.1155/2022/3169500 |
| spellingShingle | Rayhana Begum Sonia Akther Papia Mst Marium Begum Hongbin Wang Rubaba Karim Rebeka Sultana Priyanka Rani Das Taslima Begum Md. Ragibul Islam Nargis Manwar Md. Sohanur Rahman Evaluation of Hepatoprotective Potential of Polyherbal Preparations in CCl4-Induced Hepatotoxicity in Mice Advances in Pharmacological and Pharmaceutical Sciences |
| title | Evaluation of Hepatoprotective Potential of Polyherbal Preparations in CCl4-Induced Hepatotoxicity in Mice |
| title_full | Evaluation of Hepatoprotective Potential of Polyherbal Preparations in CCl4-Induced Hepatotoxicity in Mice |
| title_fullStr | Evaluation of Hepatoprotective Potential of Polyherbal Preparations in CCl4-Induced Hepatotoxicity in Mice |
| title_full_unstemmed | Evaluation of Hepatoprotective Potential of Polyherbal Preparations in CCl4-Induced Hepatotoxicity in Mice |
| title_short | Evaluation of Hepatoprotective Potential of Polyherbal Preparations in CCl4-Induced Hepatotoxicity in Mice |
| title_sort | evaluation of hepatoprotective potential of polyherbal preparations in ccl4 induced hepatotoxicity in mice |
| url | http://dx.doi.org/10.1155/2022/3169500 |
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