N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells
Peripheral nerve injuries are typically caused by either trauma or medical disorders, and recently, stem cell-based therapies have provided a promising treatment approach. Menstrual blood-derived endometrial stem cells (MenSCs) are considered an ideal therapeutic option for peripheral nerve repair d...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/3250379 |
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| _version_ | 1832562625505918976 |
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| author | Yanli Liu Fen Yang Shengying Liang Qing Liu Sulei Fu Zhenyu Wang Ciqing Yang Juntang Lin |
| author_facet | Yanli Liu Fen Yang Shengying Liang Qing Liu Sulei Fu Zhenyu Wang Ciqing Yang Juntang Lin |
| author_sort | Yanli Liu |
| collection | DOAJ |
| description | Peripheral nerve injuries are typically caused by either trauma or medical disorders, and recently, stem cell-based therapies have provided a promising treatment approach. Menstrual blood-derived endometrial stem cells (MenSCs) are considered an ideal therapeutic option for peripheral nerve repair due to a noninvasive collection procedure and their high proliferation rate and immunological tolerance. Here, we successfully isolated MenSCs and examined their biological characteristics including their morphology, multipotency, and immunophenotype. Subsequent in vitro studies demonstrated that MenSCs express high levels of neurotrophic factors, such as NT3, NT4, BDNF, and NGF, and are capable of transdifferentiating into glial-like cells under conventional induction conditions. Moreover, upregulation of N-cadherin (N-cad) mRNA and protein expression was observed after neurogenic differentiation. In vivo studies clearly showed that N-cad knockdown via in utero electroporation perturbed the migration and maturation of mouse neural precursor cells (NPCs). Finally, a further transfection assay also confirmed that N-cad upregulation in MenSCs results in the expression of S100. Collectively, our results confirmed the paracrine effect of MenSCs on neuroprotection as well as their potential for transdifferentiation into glial-like cells and demonstrated that N-cad upregulation promotes the neurogenic differentiation of MenSCs, thereby providing support for transgenic MenSC-based therapy for peripheral nerve injury. |
| format | Article |
| id | doaj-art-9dc9b51b10f24e57bd52cdc244be7b5a |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-9dc9b51b10f24e57bd52cdc244be7b5a2025-02-03T01:22:10ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/32503793250379N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem CellsYanli Liu0Fen Yang1Shengying Liang2Qing Liu3Sulei Fu4Zhenyu Wang5Ciqing Yang6Juntang Lin7Stem Cell Research Center, College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, ChinaStem Cell Research Center, College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, ChinaHenan Key Laboratory of Medical Tissue Regeneration, Xinxiang 453003, ChinaStem Cell Research Center, College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, ChinaCollege of Biomedical Engineering, Xinxiang Medical University, Xinxiang 453003, ChinaSchool of Biological and Chemical Engineering, Liaoning Institute of Science and Technology, Benxi 117004, ChinaStem Cell Research Center, College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, ChinaStem Cell Research Center, College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, ChinaPeripheral nerve injuries are typically caused by either trauma or medical disorders, and recently, stem cell-based therapies have provided a promising treatment approach. Menstrual blood-derived endometrial stem cells (MenSCs) are considered an ideal therapeutic option for peripheral nerve repair due to a noninvasive collection procedure and their high proliferation rate and immunological tolerance. Here, we successfully isolated MenSCs and examined their biological characteristics including their morphology, multipotency, and immunophenotype. Subsequent in vitro studies demonstrated that MenSCs express high levels of neurotrophic factors, such as NT3, NT4, BDNF, and NGF, and are capable of transdifferentiating into glial-like cells under conventional induction conditions. Moreover, upregulation of N-cadherin (N-cad) mRNA and protein expression was observed after neurogenic differentiation. In vivo studies clearly showed that N-cad knockdown via in utero electroporation perturbed the migration and maturation of mouse neural precursor cells (NPCs). Finally, a further transfection assay also confirmed that N-cad upregulation in MenSCs results in the expression of S100. Collectively, our results confirmed the paracrine effect of MenSCs on neuroprotection as well as their potential for transdifferentiation into glial-like cells and demonstrated that N-cad upregulation promotes the neurogenic differentiation of MenSCs, thereby providing support for transgenic MenSC-based therapy for peripheral nerve injury.http://dx.doi.org/10.1155/2018/3250379 |
| spellingShingle | Yanli Liu Fen Yang Shengying Liang Qing Liu Sulei Fu Zhenyu Wang Ciqing Yang Juntang Lin N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells Stem Cells International |
| title | N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells |
| title_full | N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells |
| title_fullStr | N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells |
| title_full_unstemmed | N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells |
| title_short | N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells |
| title_sort | n cadherin upregulation promotes the neurogenic differentiation of menstrual blood derived endometrial stem cells |
| url | http://dx.doi.org/10.1155/2018/3250379 |
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