The Alteration and Clinical Significance of Th22/Th17/Th1 Cells in Patients with Chronic Myeloid Leukemia
T helper- (Th-) cell immunodeficiency plays important roles in tumor development and their effects in chronic myeloid leukemia (CML) remain unclear. In the present study, we mainly investigated the role of Th22, Th17, and Th1 cell and their related cytokines (IL-22, IL-17, and IFN-r) in the pathophy...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/416123 |
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| author | Ping Chen Min Wang Daqi Li Yan Jia Na He Wei Li Daoxin Ma Chunyan Ji |
| author_facet | Ping Chen Min Wang Daqi Li Yan Jia Na He Wei Li Daoxin Ma Chunyan Ji |
| author_sort | Ping Chen |
| collection | DOAJ |
| description | T helper- (Th-) cell immunodeficiency plays important roles in tumor development and their effects in chronic myeloid leukemia (CML) remain unclear. In the present study, we mainly investigated the role of Th22, Th17, and Th1 cell and their related cytokines (IL-22, IL-17, and IFN-r) in the pathophysiology of CML. Bone marrow (BM) and peripheral blood (PB) were extracted from newly diagnosed (ND), chronic phase- (CP-) CML patients, and controls. Th subsets were examined by flow cytometry. Plasma IL-22, IL-17, and IFN-r concentrations were measured by ELISA. AHR and RORC mRNA expressions were examined by RT-PCR. The frequencies of Th22, Th17, and Th1 cells, along with the expression of specific transcription factors RORC and AHR, were significantly decreased in ND patients compared with healthy controls, while all these abnormality recovered in CP patients. In addition, there existed a significantly positive relationship between Th22 and Th17 cells in PB or BM. A significantly negative relationship was found between Th cells (Th22, Th17, or Th1) and BCR-ABL (%) IS or the number of PB white blood cells. All these results demonstrated that Th22, Th17, and Th1 cells might be important therapeutic targets in CML and could facilitate a better outcome for tumor immunotherapy. |
| format | Article |
| id | doaj-art-9d7f3029bc604f71befba89828e1f9bf |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-9d7f3029bc604f71befba89828e1f9bf2025-02-03T01:04:51ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/416123416123The Alteration and Clinical Significance of Th22/Th17/Th1 Cells in Patients with Chronic Myeloid LeukemiaPing Chen0Min Wang1Daqi Li2Yan Jia3Na He4Wei Li5Daoxin Ma6Chunyan Ji7Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, ChinaDepartment of Hematology, Qilu Hospital, Shandong University, Jinan 250012, ChinaDepartment of Hematology, Jinan Central Hospital, Shandong University, Jinan 250013, ChinaDepartment of Hematology, Qilu Hospital, Shandong University, Jinan 250012, ChinaDepartment of Hematology, Qilu Hospital, Shandong University, Jinan 250012, ChinaDepartment of Hematology, Qilu Hospital, Shandong University, Jinan 250012, ChinaDepartment of Hematology, Qilu Hospital, Shandong University, Jinan 250012, ChinaDepartment of Hematology, Qilu Hospital, Shandong University, Jinan 250012, ChinaT helper- (Th-) cell immunodeficiency plays important roles in tumor development and their effects in chronic myeloid leukemia (CML) remain unclear. In the present study, we mainly investigated the role of Th22, Th17, and Th1 cell and their related cytokines (IL-22, IL-17, and IFN-r) in the pathophysiology of CML. Bone marrow (BM) and peripheral blood (PB) were extracted from newly diagnosed (ND), chronic phase- (CP-) CML patients, and controls. Th subsets were examined by flow cytometry. Plasma IL-22, IL-17, and IFN-r concentrations were measured by ELISA. AHR and RORC mRNA expressions were examined by RT-PCR. The frequencies of Th22, Th17, and Th1 cells, along with the expression of specific transcription factors RORC and AHR, were significantly decreased in ND patients compared with healthy controls, while all these abnormality recovered in CP patients. In addition, there existed a significantly positive relationship between Th22 and Th17 cells in PB or BM. A significantly negative relationship was found between Th cells (Th22, Th17, or Th1) and BCR-ABL (%) IS or the number of PB white blood cells. All these results demonstrated that Th22, Th17, and Th1 cells might be important therapeutic targets in CML and could facilitate a better outcome for tumor immunotherapy.http://dx.doi.org/10.1155/2015/416123 |
| spellingShingle | Ping Chen Min Wang Daqi Li Yan Jia Na He Wei Li Daoxin Ma Chunyan Ji The Alteration and Clinical Significance of Th22/Th17/Th1 Cells in Patients with Chronic Myeloid Leukemia Journal of Immunology Research |
| title | The Alteration and Clinical Significance of Th22/Th17/Th1 Cells in Patients with Chronic Myeloid Leukemia |
| title_full | The Alteration and Clinical Significance of Th22/Th17/Th1 Cells in Patients with Chronic Myeloid Leukemia |
| title_fullStr | The Alteration and Clinical Significance of Th22/Th17/Th1 Cells in Patients with Chronic Myeloid Leukemia |
| title_full_unstemmed | The Alteration and Clinical Significance of Th22/Th17/Th1 Cells in Patients with Chronic Myeloid Leukemia |
| title_short | The Alteration and Clinical Significance of Th22/Th17/Th1 Cells in Patients with Chronic Myeloid Leukemia |
| title_sort | alteration and clinical significance of th22 th17 th1 cells in patients with chronic myeloid leukemia |
| url | http://dx.doi.org/10.1155/2015/416123 |
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