<i>Ajuga reptans</i> L. Herb Extracts: Phytochemical Composition and Pharmacological Activity Screening
The genus <i>Ajuga</i> (Lamiaceae family) comprises approximately 300 species, which are widely used in traditional medicine for their diaphoretic, antiseptic, hemostatic, and anti-inflammatory properties, but scarcely in official ones. Therefore, the study of <i>Ajuga reptans</...
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2025-01-01
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| author | Svitlana Maliuvanchuk Andriy Grytsyk Oksana Popadynets Taras Kotyk Ain Raal Oleh Koshovyi |
| author_facet | Svitlana Maliuvanchuk Andriy Grytsyk Oksana Popadynets Taras Kotyk Ain Raal Oleh Koshovyi |
| author_sort | Svitlana Maliuvanchuk |
| collection | DOAJ |
| description | The genus <i>Ajuga</i> (Lamiaceae family) comprises approximately 300 species, which are widely used in traditional medicine for their diaphoretic, antiseptic, hemostatic, and anti-inflammatory properties, but scarcely in official ones. Therefore, the study of <i>Ajuga reptans</i> holds promise for developing new medicinal products. In aqueous and aqueous-alcoholic soft extracts of the <i>A. reptans</i> herb, 16 amino acids, 20 phenolics, and 10 volatile substances were identified by HPLC and GC/MS. The assays of the main substances’ groups were also determined by spectrophotometry (vitamin K1, polyphenols, tannins, flavonoids, and hydroxycinnamic acids) and titrometry (ascorbic and organic acids). <i>A. reptans</i> herb extracts are practically non-toxic, exhibit hepatoprotective activity (dose 25 mg/kg) in experimental carbon tetrachloride-induced hepatitis, moderate anti-inflammatory activity (dose 100 mg/kg) in carrageenan-induced edema models, and possess significant local hemostatic (reducing bleeding time by 40.6%) and wound-healing properties (complete wound healing after 9 days). The aqueous-ethanolic soft <i>A. reptans</i> extract (extractant 50% ethanol) demonstrated the most pronounced hepatoprotective and anti-inflammatory effects. <i>A. reptans</i> extracts are capable of inhibiting the growth of microorganisms and showing higher activity against Gram-positive bacteria. <i>A. reptans</i> herb extracts are promising agents for implementation in official medicine as wound healing and hepatoprotective remedies after further preclinical and clinical studies. |
| format | Article |
| id | doaj-art-9d7db225d9ff4e66abbc15c3984eec71 |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-9d7db225d9ff4e66abbc15c3984eec712025-01-24T13:46:48ZengMDPI AGPlants2223-77472025-01-0114221910.3390/plants14020219<i>Ajuga reptans</i> L. Herb Extracts: Phytochemical Composition and Pharmacological Activity ScreeningSvitlana Maliuvanchuk0Andriy Grytsyk1Oksana Popadynets2Taras Kotyk3Ain Raal4Oleh Koshovyi5Department of Pharmaceutical Management, Drug Technology and Pharmacognosy, Ivano-Frankivsk National Medical University, 76000 Ivano-Frankivsk, UkraineDepartment of Pharmaceutical Management, Drug Technology and Pharmacognosy, Ivano-Frankivsk National Medical University, 76000 Ivano-Frankivsk, UkraineDepartment of Anatomy, Ivano-Frankivsk National Medical University, 76000 Ivano-Frankivsk, UkraineDepartment of Anatomy, Ivano-Frankivsk National Medical University, 76000 Ivano-Frankivsk, UkraineInstitute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, EstoniaInstitute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, EstoniaThe genus <i>Ajuga</i> (Lamiaceae family) comprises approximately 300 species, which are widely used in traditional medicine for their diaphoretic, antiseptic, hemostatic, and anti-inflammatory properties, but scarcely in official ones. Therefore, the study of <i>Ajuga reptans</i> holds promise for developing new medicinal products. In aqueous and aqueous-alcoholic soft extracts of the <i>A. reptans</i> herb, 16 amino acids, 20 phenolics, and 10 volatile substances were identified by HPLC and GC/MS. The assays of the main substances’ groups were also determined by spectrophotometry (vitamin K1, polyphenols, tannins, flavonoids, and hydroxycinnamic acids) and titrometry (ascorbic and organic acids). <i>A. reptans</i> herb extracts are practically non-toxic, exhibit hepatoprotective activity (dose 25 mg/kg) in experimental carbon tetrachloride-induced hepatitis, moderate anti-inflammatory activity (dose 100 mg/kg) in carrageenan-induced edema models, and possess significant local hemostatic (reducing bleeding time by 40.6%) and wound-healing properties (complete wound healing after 9 days). The aqueous-ethanolic soft <i>A. reptans</i> extract (extractant 50% ethanol) demonstrated the most pronounced hepatoprotective and anti-inflammatory effects. <i>A. reptans</i> extracts are capable of inhibiting the growth of microorganisms and showing higher activity against Gram-positive bacteria. <i>A. reptans</i> herb extracts are promising agents for implementation in official medicine as wound healing and hepatoprotective remedies after further preclinical and clinical studies.https://www.mdpi.com/2223-7747/14/2/219<i>Ajuga reptans</i> L.extractphenolic compoundshepatoprotective activityhemostatic activitywound-healing activity |
| spellingShingle | Svitlana Maliuvanchuk Andriy Grytsyk Oksana Popadynets Taras Kotyk Ain Raal Oleh Koshovyi <i>Ajuga reptans</i> L. Herb Extracts: Phytochemical Composition and Pharmacological Activity Screening Plants <i>Ajuga reptans</i> L. extract phenolic compounds hepatoprotective activity hemostatic activity wound-healing activity |
| title | <i>Ajuga reptans</i> L. Herb Extracts: Phytochemical Composition and Pharmacological Activity Screening |
| title_full | <i>Ajuga reptans</i> L. Herb Extracts: Phytochemical Composition and Pharmacological Activity Screening |
| title_fullStr | <i>Ajuga reptans</i> L. Herb Extracts: Phytochemical Composition and Pharmacological Activity Screening |
| title_full_unstemmed | <i>Ajuga reptans</i> L. Herb Extracts: Phytochemical Composition and Pharmacological Activity Screening |
| title_short | <i>Ajuga reptans</i> L. Herb Extracts: Phytochemical Composition and Pharmacological Activity Screening |
| title_sort | i ajuga reptans i l herb extracts phytochemical composition and pharmacological activity screening |
| topic | <i>Ajuga reptans</i> L. extract phenolic compounds hepatoprotective activity hemostatic activity wound-healing activity |
| url | https://www.mdpi.com/2223-7747/14/2/219 |
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