Phenotypic Timeline Kinetics, Integrative Networks, and Performance of T- and B-Cell Subsets Associated with Distinct Clinical Outcome of Severe COVID-19 Patients

Phenotypic Timeline Kinetics, Integrative Networks, and Performance of T- and B-Cell Subsets Associated with Distinct Clinical Outcome of Severe COVID-19 Patients

The present study aimed to evaluate the kinetics of the phenotypic profile and integrative networks of T/B-cells in severe COVID-19 patients, categorized according to disease outcome, during the circulation of the B.1.1.28 and B.1.1.33 SARS-CoV-2 strains in Brazil. Peripheral blood obtained at disti...

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Main Authors: Gabriela de Oliveira, Ismael Artur Costa-Rocha, Nani Oliveira-Carvalho, Tâmilla Mayane Alves Fidelis dos Santos, Ana Carolina Campi-Azevedo, Vanessa Peruhype-Magalhães, Vitor Hugo Simões Miranda, Roberta Oliveira Prado, Agnes Antônia Sampaio Pereira, Clarice Carvalho Alves, Joaquim Pedro Brito-de-Sousa, Laise Rodrigues Reis, Christiane Costa-Pereira, Camila Pacheco Silveira Martins da Mata, Vanessa Egídio Silveira Almeida, Liliane Martins dos Santos, Gregório Guilherme Almeida, Lis Ribeiro do Valle Antonelli, Jordana Grazziela Coelho-dos-Reis, Andréa Teixeira-Carvalho, Olindo Assis Martins-Filho
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Microorganisms
Subjects:
COVID-19
disease outcome
cellular memory
activation
exhaustion
Online Access:https://www.mdpi.com/2076-2607/12/11/2272
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Summary:The present study aimed to evaluate the kinetics of the phenotypic profile and integrative networks of T/B-cells in severe COVID-19 patients, categorized according to disease outcome, during the circulation of the B.1.1.28 and B.1.1.33 SARS-CoV-2 strains in Brazil. Peripheral blood obtained at distinct time points (baseline/D0; D7; D14-28) was used for ex vivo flow cytometry immunophenotyping. The data demonstrated a decrease at D0 in the frequency of CD3<sup>+</sup> T-cells and CD4<sup>+</sup> T-cells and an increase in B-cells with mixed activation/exhaustion profiles. Higher changes in B-cell and CD4<sup>+</sup> T-cells at D7 were associated with discharge/death outcomes, respectively. Regardless of the lower T/B-cell connectivity at D0, distinct profiles from D7/D14-28 revealed that, while discharge was associated with increasing connectivity for B-cells, CD4<sup>+</sup> and CD8<sup>+</sup> T-cells death was related to increased connectivity involving B-cells, but with lower connections mediated by CD4<sup>+</sup> T-cells. The CD4<sup>+</sup>CD38<sup>+</sup> and CD8<sup>+</sup>CD69<sup>+</sup> subsets accurately classified COVID-19 vs. healthy controls throughout the kinetic analysis. Binary logistic regression identified CD4<sup>+</sup>CD107a<sup>+</sup>, CD4<sup>+</sup>T-bet<sup>+</sup>, CD8<sup>+</sup>CD69<sup>+</sup>, and CD8<sup>+</sup>T-bet<sup>+</sup> at D0 and CD4<sup>+</sup>CD45RO<sup>+</sup>CD27<sup>+</sup> at D7 as subsets associated with disease outcomes. Results showed that distinct phenotypic timeline kinetics and integrative networks of T/B-cells are associated with COVID-19 outcomes that may subsidize the establishment of applicable biomarkers for clinical/therapeutic monitoring.
ISSN:2076-2607

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