Genetic diversity atlas of Brucella melitensis strains from Sichuan Province, China
Abstract Human brucellosis is a re-emerging disease in Sichuan Province, China. In this study, bacteriology, conventional bio-typing, multi-locus sequence typing (MLST), and multiple locus variable-number tandem repeat analysis (MLVA) were applied to preliminarily characterize the strains in terms o...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12866-024-03739-x |
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| author | Wenbo Li Linzi Zeng Rongmei Yuan Teng Qi Hongyu Liao Yuhangxi Cao Shu Huang Zhiguo Liu Zhenjun Li |
| author_facet | Wenbo Li Linzi Zeng Rongmei Yuan Teng Qi Hongyu Liao Yuhangxi Cao Shu Huang Zhiguo Liu Zhenjun Li |
| author_sort | Wenbo Li |
| collection | DOAJ |
| description | Abstract Human brucellosis is a re-emerging disease in Sichuan Province, China. In this study, bacteriology, conventional bio-typing, multi-locus sequence typing (MLST), and multiple locus variable-number tandem repeat analysis (MLVA) were applied to preliminarily characterize the strains in terms of genetic diversity and epidemiological links. A total of 101 Brucella strains were isolated from 16 cities (autonomous prefectures) from 2014 to 2021, and all of the strains were identified as Brucella melitensis bv. 3, suggesting that surveillance should focus on ruminants. MLST analysis identified four STs, namely, ST8 (n = 93), ST39 (n = 6), ST101 (n = 1), and ST118 (n = 1). The latter were new STs, indicating that strains displayed high population diversity. Six MLVA-8, namely, 42, 43, 45, 63, 83, and 114, and eight MLVA-11, namely, 111, 115, 116, 125, 180, 291, 298, and 342, genotypes were identified, demonstrating that all of the strains were from the Eastern Mediterranean lineage, and these strains exhibited a high genotype diversity. MLVA-16 analysis revealed that there was a co-existing transmission pattern, where sporadic cases and multiple outbreak events had a common origin. The dominant STs and MLVA genotypes of strains were epidemic in Northern, China, and 36 MLVA-16 genotypes were shared among strains (n = 51, 50.4%, 51/101) from Sichuan and strains from 22 other provinces. The findings imply that infected animals were introduced from outside the province. The surveillance and control of the disease have become public health challenges. Animal quarantines should be strengthened to prevent the spread of B. melitensis species among adjacent regions. |
| format | Article |
| id | doaj-art-9cc385bea99f4e868d2f81f40d5ec40f |
| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj-art-9cc385bea99f4e868d2f81f40d5ec40f2025-01-19T12:12:28ZengBMCBMC Microbiology1471-21802025-01-0125111010.1186/s12866-024-03739-xGenetic diversity atlas of Brucella melitensis strains from Sichuan Province, ChinaWenbo Li0Linzi Zeng1Rongmei Yuan2Teng Qi3Hongyu Liao4Yuhangxi Cao5Shu Huang6Zhiguo Liu7Zhenjun Li8Sichuan Provincial Center for Disease Control and PreventionSichuan Provincial Center for Disease Control and PreventionSichuan Provincial Center for Disease Control and PreventionSichuan Provincial Center for Disease Control and PreventionSichuan Provincial Center for Disease Control and PreventionSichuan Provincial Center for Disease Control and PreventionSichuan Provincial Center for Disease Control and PreventionNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionAbstract Human brucellosis is a re-emerging disease in Sichuan Province, China. In this study, bacteriology, conventional bio-typing, multi-locus sequence typing (MLST), and multiple locus variable-number tandem repeat analysis (MLVA) were applied to preliminarily characterize the strains in terms of genetic diversity and epidemiological links. A total of 101 Brucella strains were isolated from 16 cities (autonomous prefectures) from 2014 to 2021, and all of the strains were identified as Brucella melitensis bv. 3, suggesting that surveillance should focus on ruminants. MLST analysis identified four STs, namely, ST8 (n = 93), ST39 (n = 6), ST101 (n = 1), and ST118 (n = 1). The latter were new STs, indicating that strains displayed high population diversity. Six MLVA-8, namely, 42, 43, 45, 63, 83, and 114, and eight MLVA-11, namely, 111, 115, 116, 125, 180, 291, 298, and 342, genotypes were identified, demonstrating that all of the strains were from the Eastern Mediterranean lineage, and these strains exhibited a high genotype diversity. MLVA-16 analysis revealed that there was a co-existing transmission pattern, where sporadic cases and multiple outbreak events had a common origin. The dominant STs and MLVA genotypes of strains were epidemic in Northern, China, and 36 MLVA-16 genotypes were shared among strains (n = 51, 50.4%, 51/101) from Sichuan and strains from 22 other provinces. The findings imply that infected animals were introduced from outside the province. The surveillance and control of the disease have become public health challenges. Animal quarantines should be strengthened to prevent the spread of B. melitensis species among adjacent regions.https://doi.org/10.1186/s12866-024-03739-xBrucella melitensisBio-typingMLSTMLVAGenetic diversity |
| spellingShingle | Wenbo Li Linzi Zeng Rongmei Yuan Teng Qi Hongyu Liao Yuhangxi Cao Shu Huang Zhiguo Liu Zhenjun Li Genetic diversity atlas of Brucella melitensis strains from Sichuan Province, China BMC Microbiology Brucella melitensis Bio-typing MLST MLVA Genetic diversity |
| title | Genetic diversity atlas of Brucella melitensis strains from Sichuan Province, China |
| title_full | Genetic diversity atlas of Brucella melitensis strains from Sichuan Province, China |
| title_fullStr | Genetic diversity atlas of Brucella melitensis strains from Sichuan Province, China |
| title_full_unstemmed | Genetic diversity atlas of Brucella melitensis strains from Sichuan Province, China |
| title_short | Genetic diversity atlas of Brucella melitensis strains from Sichuan Province, China |
| title_sort | genetic diversity atlas of brucella melitensis strains from sichuan province china |
| topic | Brucella melitensis Bio-typing MLST MLVA Genetic diversity |
| url | https://doi.org/10.1186/s12866-024-03739-x |
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