Nuclear Intron Sequence Variation of the <i>Bulinus globosus</i> Complex (Mollusca: Planorbidae): Implications for Molecular Systematic Analyses
Urinary schistosomiasis is caused by the blood fluke <i>Schistosoma haematobium</i>, which is predominantly found in Africa. The freshwater snail <i>Bulinus globosus</i> is its main intermediate host. The species that make up the <i>B. globosus</i> group are genet...
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2025-01-01
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| author | Chairat Tantrawatpan Kotchaphon Vaisusuk Chrysantus M. Tanga Warayutt Pilap Naruemon Bunchom Ross H. Andrews Tongjit Thanchomnang Wanchai Maleewong Weerachai Saijuntha |
| author_facet | Chairat Tantrawatpan Kotchaphon Vaisusuk Chrysantus M. Tanga Warayutt Pilap Naruemon Bunchom Ross H. Andrews Tongjit Thanchomnang Wanchai Maleewong Weerachai Saijuntha |
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| description | Urinary schistosomiasis is caused by the blood fluke <i>Schistosoma haematobium</i>, which is predominantly found in Africa. The freshwater snail <i>Bulinus globosus</i> is its main intermediate host. The species that make up the <i>B. globosus</i> group are genetically complex, and their taxonomic status remains controversial. Genetic variation, heterozygosity, and DNA recombination in <i>B. globosus</i> were examined using the mitochondrial cytochrome c oxidase subunit 1 (<i>COI</i>) and the intron 3 region of the arginine kinase gene (AkInt3). A total of 81 <i>B. globosus</i> snails were collected from three different localities in Kwale County, Kenya. Genomic diversity, heterozygosity, DNA recombination, and haplotype network were calculated using AkInt3 sequences. Low polymorphism in the <i>COI</i> sequence divided <i>B. globosus</i> into six haplotypes (C1–C6). However, AkInt3 sequencing studies showed high polymorphisms, classifying 81 <i>B. globosus</i> snails into 44 haplotypes (H1–H44). These haplotypes were separated into three haplogroups (I–III). AkInt3 sequence heterozygosity was also found. DNA recombination haplotypes between haplogroups were commonly found in heterozygous samples. AkInt3 sequence studies showed high levels of genetic polymorphism and heterozygosity, supporting its use as a genetic marker for elucidating the population genetics of <i>B. globosus</i>. Furthermore, our study showed that <i>B. globosus</i> populations in Kenya form a “species complex”. |
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| institution | Kabale University |
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| language | English |
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| spelling | doaj-art-9c5efabd35d245a59a3e98073b1715652025-01-24T13:23:26ZengMDPI AGBiology2079-77372025-01-011415310.3390/biology14010053Nuclear Intron Sequence Variation of the <i>Bulinus globosus</i> Complex (Mollusca: Planorbidae): Implications for Molecular Systematic AnalysesChairat Tantrawatpan0Kotchaphon Vaisusuk1Chrysantus M. Tanga2Warayutt Pilap3Naruemon Bunchom4Ross H. Andrews5Tongjit Thanchomnang6Wanchai Maleewong7Weerachai Saijuntha8Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, and Center of Excellence in Stem Cell Research and Innovation, Thammasat University, Rangsit Campus, Pathum Thani 12120, ThailandDepartment of Veterinary Technology, Faculty of Agricultural Technology, Rajabhat Maha Sarakham University, Maha Sarakham 44000, ThailandPlant Health Theme, International Centre of Insect Physiology and Ecology, Nairobi 00100, KenyaWalai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, ThailandDepartment of Tropical Medicine and Malaria, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku-Ku, Tokyo 162-8655, JapanDepartment of Surgery & Cancer, Faculty of Medicine, Imperial College London, South Kensington Campus, London SW7 2AZ, UKBiomedical Science Research Unit, Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, ThailandDepartment of Parasitology, Faculty of Medicine, and Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, ThailandBiomedical Science Research Unit, Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, ThailandUrinary schistosomiasis is caused by the blood fluke <i>Schistosoma haematobium</i>, which is predominantly found in Africa. The freshwater snail <i>Bulinus globosus</i> is its main intermediate host. The species that make up the <i>B. globosus</i> group are genetically complex, and their taxonomic status remains controversial. Genetic variation, heterozygosity, and DNA recombination in <i>B. globosus</i> were examined using the mitochondrial cytochrome c oxidase subunit 1 (<i>COI</i>) and the intron 3 region of the arginine kinase gene (AkInt3). A total of 81 <i>B. globosus</i> snails were collected from three different localities in Kwale County, Kenya. Genomic diversity, heterozygosity, DNA recombination, and haplotype network were calculated using AkInt3 sequences. Low polymorphism in the <i>COI</i> sequence divided <i>B. globosus</i> into six haplotypes (C1–C6). However, AkInt3 sequencing studies showed high polymorphisms, classifying 81 <i>B. globosus</i> snails into 44 haplotypes (H1–H44). These haplotypes were separated into three haplogroups (I–III). AkInt3 sequence heterozygosity was also found. DNA recombination haplotypes between haplogroups were commonly found in heterozygous samples. AkInt3 sequence studies showed high levels of genetic polymorphism and heterozygosity, supporting its use as a genetic marker for elucidating the population genetics of <i>B. globosus</i>. Furthermore, our study showed that <i>B. globosus</i> populations in Kenya form a “species complex”.https://www.mdpi.com/2079-7737/14/1/53pulmonate snailintermediate hostschistosomiasisheterozygositygenetic variationDNA recombination |
| spellingShingle | Chairat Tantrawatpan Kotchaphon Vaisusuk Chrysantus M. Tanga Warayutt Pilap Naruemon Bunchom Ross H. Andrews Tongjit Thanchomnang Wanchai Maleewong Weerachai Saijuntha Nuclear Intron Sequence Variation of the <i>Bulinus globosus</i> Complex (Mollusca: Planorbidae): Implications for Molecular Systematic Analyses Biology pulmonate snail intermediate host schistosomiasis heterozygosity genetic variation DNA recombination |
| title | Nuclear Intron Sequence Variation of the <i>Bulinus globosus</i> Complex (Mollusca: Planorbidae): Implications for Molecular Systematic Analyses |
| title_full | Nuclear Intron Sequence Variation of the <i>Bulinus globosus</i> Complex (Mollusca: Planorbidae): Implications for Molecular Systematic Analyses |
| title_fullStr | Nuclear Intron Sequence Variation of the <i>Bulinus globosus</i> Complex (Mollusca: Planorbidae): Implications for Molecular Systematic Analyses |
| title_full_unstemmed | Nuclear Intron Sequence Variation of the <i>Bulinus globosus</i> Complex (Mollusca: Planorbidae): Implications for Molecular Systematic Analyses |
| title_short | Nuclear Intron Sequence Variation of the <i>Bulinus globosus</i> Complex (Mollusca: Planorbidae): Implications for Molecular Systematic Analyses |
| title_sort | nuclear intron sequence variation of the i bulinus globosus i complex mollusca planorbidae implications for molecular systematic analyses |
| topic | pulmonate snail intermediate host schistosomiasis heterozygosity genetic variation DNA recombination |
| url | https://www.mdpi.com/2079-7737/14/1/53 |
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