Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological Effects
Aim. The focal theme of present investigation includes isolation of prodigiosin producing fish gut bacteria, enhancing its production using tannery solid waste fleshing, and evaluation of its pharmacological effect. Methods. Optimization of fermentation conditions to yield maximum prodigiosin, and i...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/290327 |
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| author | C. Sumathi D. MohanaPriya S. Swarnalatha M. G. Dinesh G. Sekaran |
| author_facet | C. Sumathi D. MohanaPriya S. Swarnalatha M. G. Dinesh G. Sekaran |
| author_sort | C. Sumathi |
| collection | DOAJ |
| description | Aim. The focal theme of present investigation includes isolation of prodigiosin producing fish gut bacteria, enhancing its production using tannery solid waste fleshing, and evaluation of its pharmacological effect. Methods. Optimization of fermentation conditions to yield maximum prodigiosin, and instrumental analysis using FTIR, NMR, ESI-MS, TGA, and DSC. Results. The optimum conditions required for the maximum prodigiosin concentration were achieved at time 30 h, temperature 30°C, pH 8, and 3% substrate concentration. The secondary metabolite was analyzed using ESI-MS, FTIR, and NMR. Therapeutic efficacy was assessed by in vitro anticancer studies. Among the pathogenic bacteria Pseudomonas aeruginosa was most susceptible at the lowest concentration followed by Salmonella typhi. IC50 concentration was cell line specific (HeLa cells: 4.3 µM, HEp2: 5.2 µM, and KB cells: 4.8 µM) and remains nontoxic up to the concentration of 25 µM on normal Vero cells suggesting that cancerous cells are more susceptible to the prodigiosin at lower concentration. Conclusion. Maximum prodigiosin production was obtained with tannery fleshing. The potency of the fish gut bacterial secondary metabolite prodigiosin as a therapeutic agent was confirmed through in vitro antimicrobial and anticancer studies. |
| format | Article |
| id | doaj-art-9bb18e5cce4247f89956e32b2811e78f |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-9bb18e5cce4247f89956e32b2811e78f2025-02-03T06:44:25ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/290327290327Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological EffectsC. Sumathi0D. MohanaPriya1S. Swarnalatha2M. G. Dinesh3G. Sekaran4Council of Scientific and Industrial Research (CSIR), Central Leather Research Institute (CLRI), Adyar, Chennai, Tamil Nadu 600020, IndiaTamil Nadu Veterinary and Animal Sciences University, Chennai, IndiaNational Institute of Environmental Research (Ministry of Environment), Incheon, Republic of KoreaSri Ramachandra University, Chennai, IndiaCouncil of Scientific and Industrial Research (CSIR), Central Leather Research Institute (CLRI), Adyar, Chennai, Tamil Nadu 600020, IndiaAim. The focal theme of present investigation includes isolation of prodigiosin producing fish gut bacteria, enhancing its production using tannery solid waste fleshing, and evaluation of its pharmacological effect. Methods. Optimization of fermentation conditions to yield maximum prodigiosin, and instrumental analysis using FTIR, NMR, ESI-MS, TGA, and DSC. Results. The optimum conditions required for the maximum prodigiosin concentration were achieved at time 30 h, temperature 30°C, pH 8, and 3% substrate concentration. The secondary metabolite was analyzed using ESI-MS, FTIR, and NMR. Therapeutic efficacy was assessed by in vitro anticancer studies. Among the pathogenic bacteria Pseudomonas aeruginosa was most susceptible at the lowest concentration followed by Salmonella typhi. IC50 concentration was cell line specific (HeLa cells: 4.3 µM, HEp2: 5.2 µM, and KB cells: 4.8 µM) and remains nontoxic up to the concentration of 25 µM on normal Vero cells suggesting that cancerous cells are more susceptible to the prodigiosin at lower concentration. Conclusion. Maximum prodigiosin production was obtained with tannery fleshing. The potency of the fish gut bacterial secondary metabolite prodigiosin as a therapeutic agent was confirmed through in vitro antimicrobial and anticancer studies.http://dx.doi.org/10.1155/2014/290327 |
| spellingShingle | C. Sumathi D. MohanaPriya S. Swarnalatha M. G. Dinesh G. Sekaran Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological Effects The Scientific World Journal |
| title | Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological Effects |
| title_full | Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological Effects |
| title_fullStr | Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological Effects |
| title_full_unstemmed | Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological Effects |
| title_short | Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological Effects |
| title_sort | production of prodigiosin using tannery fleshing and evaluating its pharmacological effects |
| url | http://dx.doi.org/10.1155/2014/290327 |
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