Biological Characteristics Analysis of Limosilactobacillus reuteri JBR3 and the Effect of Protective Agents on Its Viability
In order to advance the application of Limosilactobacillus reuteri IMAUJBR3 in meat fermenters, the strain was subjected to comprehensive genomic, metabolic and biological analyses. Additionally, one-way and response surface experiments were conducted to identify the optimal ratio of protectants for...
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The editorial department of Science and Technology of Food Industry
2025-02-01
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| Series: | Shipin gongye ke-ji |
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| author | Jun HAN Yi WANG Kaiping ZHANG Jianjun TIAN |
| author_facet | Jun HAN Yi WANG Kaiping ZHANG Jianjun TIAN |
| author_sort | Jun HAN |
| collection | DOAJ |
| description | In order to advance the application of Limosilactobacillus reuteri IMAUJBR3 in meat fermenters, the strain was subjected to comprehensive genomic, metabolic and biological analyses. Additionally, one-way and response surface experiments were conducted to identify the optimal ratio of protectants for freeze-drying of lactic acid bacteria. The genome of JBR3 was found to consist of one circular chromosome and two circular plasmids. Five sterol esterase-producing enzyme (EC 3.1.1.13) genes, including gene0325 and gene1042, were identified in the metabolic system, which could be hydrolysed to produce sterols and fatty acids. In the probiotic characterisation, no biogenic amine-related genes were annotated. However, several chaperonin genes, such as groEL and groES, and cold stress genes, gene0733 and gene1677 were found. The presence of these genes ensured that the functional properties of the strain could be maintained during the period of cold stress in lactic acid bacteria. This suggested that the strain was suitable for freeze-drying. In addition, JBR3 contained an intact NADH system, yfeX peroxidase, gor glutathione reductase, trxA thioredoxin and trxB thioredoxin reductase. This suggested that JBR3 might have a superior antioxidant capacity, which could be exploited as a fermentation agent in fermented foods. JBR3 was freeze-dried under a protective agent complex of skimmed milk powder (9.3 g/100 mL), maltodextrin (2.3 g/100 mL), monosodium glutamate (6.8 g/100 mL), and trehalose (10.0 g/100 mL). The survival rate of the strain was 95.17%±0.57%, with a viable bacterial number of 9.20×1010 CFU/g. The strain has a promising future in various applications. |
| format | Article |
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| institution | Kabale University |
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| language | zho |
| publishDate | 2025-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| spelling | doaj-art-9b4048bdfe874dc197049af8fe10676a2025-01-21T07:24:08ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-02-0146316617710.13386/j.issn1002-0306.20240303552024030355-3Biological Characteristics Analysis of Limosilactobacillus reuteri JBR3 and the Effect of Protective Agents on Its ViabilityJun HAN0Yi WANG1Kaiping ZHANG2Jianjun TIAN3College of Food Science and Engieering, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Food Science and Engieering, Inner Mongolia Agricultural University, Hohhot 010018, ChinaDepartment of Cooking & Food Processing, Inner Mongolia Business and Trade Vocational College, Hohhot 010070, ChinaCollege of Food Science and Engieering, Inner Mongolia Agricultural University, Hohhot 010018, ChinaIn order to advance the application of Limosilactobacillus reuteri IMAUJBR3 in meat fermenters, the strain was subjected to comprehensive genomic, metabolic and biological analyses. Additionally, one-way and response surface experiments were conducted to identify the optimal ratio of protectants for freeze-drying of lactic acid bacteria. The genome of JBR3 was found to consist of one circular chromosome and two circular plasmids. Five sterol esterase-producing enzyme (EC 3.1.1.13) genes, including gene0325 and gene1042, were identified in the metabolic system, which could be hydrolysed to produce sterols and fatty acids. In the probiotic characterisation, no biogenic amine-related genes were annotated. However, several chaperonin genes, such as groEL and groES, and cold stress genes, gene0733 and gene1677 were found. The presence of these genes ensured that the functional properties of the strain could be maintained during the period of cold stress in lactic acid bacteria. This suggested that the strain was suitable for freeze-drying. In addition, JBR3 contained an intact NADH system, yfeX peroxidase, gor glutathione reductase, trxA thioredoxin and trxB thioredoxin reductase. This suggested that JBR3 might have a superior antioxidant capacity, which could be exploited as a fermentation agent in fermented foods. JBR3 was freeze-dried under a protective agent complex of skimmed milk powder (9.3 g/100 mL), maltodextrin (2.3 g/100 mL), monosodium glutamate (6.8 g/100 mL), and trehalose (10.0 g/100 mL). The survival rate of the strain was 95.17%±0.57%, with a viable bacterial number of 9.20×1010 CFU/g. The strain has a promising future in various applications.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030355limosilactobacillus reuteriwhole genomefreeze-dryingprotective agentsurvival rate |
| spellingShingle | Jun HAN Yi WANG Kaiping ZHANG Jianjun TIAN Biological Characteristics Analysis of Limosilactobacillus reuteri JBR3 and the Effect of Protective Agents on Its Viability Shipin gongye ke-ji limosilactobacillus reuteri whole genome freeze-drying protective agent survival rate |
| title | Biological Characteristics Analysis of Limosilactobacillus reuteri JBR3 and the Effect of Protective Agents on Its Viability |
| title_full | Biological Characteristics Analysis of Limosilactobacillus reuteri JBR3 and the Effect of Protective Agents on Its Viability |
| title_fullStr | Biological Characteristics Analysis of Limosilactobacillus reuteri JBR3 and the Effect of Protective Agents on Its Viability |
| title_full_unstemmed | Biological Characteristics Analysis of Limosilactobacillus reuteri JBR3 and the Effect of Protective Agents on Its Viability |
| title_short | Biological Characteristics Analysis of Limosilactobacillus reuteri JBR3 and the Effect of Protective Agents on Its Viability |
| title_sort | biological characteristics analysis of limosilactobacillus reuteri jbr3 and the effect of protective agents on its viability |
| topic | limosilactobacillus reuteri whole genome freeze-drying protective agent survival rate |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030355 |
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