Mutagenesis by retroviral insertion in chemical mutagen-generated quasi-haploid mammalian cells
Diploidy is a major obstacle to the mutagenic analysis of function in cultured mammalian cells. Here, we show that 6–8 rounds of chemical mutagenesis generates quasi-haploid cells that can be used as targets for insertional mutagenesis using a specially designed retroviral vector that permits rapid...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2007-04-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112390 |
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