Development of new powdery mildew resistant lines in garden pea (Pisum sativum L.) using induced mutagenesis and validation of resistance for the er1 and er2 gene through molecular markers
Powdery mildew (PM) caused by Erysiphie pisi Syd. is the most devastating disease of pea, affecting fresh pea production as well as the quality of the marketable harvest worldwide. The efforts were made to develop PM-resistant mutants of popular pea varieties “Lincoln” and “Azad P-1” through induced...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1501661/full |
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| author | Akhilesh Sharma Devinder Kumar Banyal Vinod Janardan Dhole Bansuli Ranbir Singh Rana Rajesh Kumar Prabhat Kumar Nimit Kumar Srishti Arshia Prashar Vivek Singh Anoushka Sharma |
| author_facet | Akhilesh Sharma Devinder Kumar Banyal Vinod Janardan Dhole Bansuli Ranbir Singh Rana Rajesh Kumar Prabhat Kumar Nimit Kumar Srishti Arshia Prashar Vivek Singh Anoushka Sharma |
| author_sort | Akhilesh Sharma |
| collection | DOAJ |
| description | Powdery mildew (PM) caused by Erysiphie pisi Syd. is the most devastating disease of pea, affecting fresh pea production as well as the quality of the marketable harvest worldwide. The efforts were made to develop PM-resistant mutants of popular pea varieties “Lincoln” and “Azad P-1” through induced mutations by following gamma irradiation (300, 400, 500, and 600 Gy) and chemical mutagenesis, i.e., ethyl methane sulfonate (EMS) (0.3% and 0.4%). The screening of 13,868 M2 progenies at Kukumseri (summer season) followed by M3 generation at Palampur (winter season) resulted in the isolation of six putative PM-resistant mutants. The rigorous evaluation of these progenies under in vivo (field screening) and in vitro (artificial screening under greenhouse conditions and using the detached leaf assay method) conditions over the years resulted in the isolation of three PM-resistant mutants, viz., L-40-1014, L-0.3-139, and AP-0.3-129. SSR markers “PSMPSAD60 d” and “PSMPA5 c” linked to the er-1 gene indicated the presence of the “er1” gene in the mutant L-0.3-139 while the er-2 gene-linked SCAR marker “ScX171400” and SSR marker “AD141” indicated the probability of the “er-2” gene in mutant L-40-1014. The known markers linked to PM resistance genes could not be validated in the mutant AP-0.3-129, suggested to identify new markers linked to PM resistance. These PM-resistant mutants can be promising candidates as the new source of resistance for future pea breeding programs. |
| format | Article |
| id | doaj-art-9aaa9d53206646e6aa4a1e2a7ee07839 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-9aaa9d53206646e6aa4a1e2a7ee078392025-01-28T06:40:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15016611501661Development of new powdery mildew resistant lines in garden pea (Pisum sativum L.) using induced mutagenesis and validation of resistance for the er1 and er2 gene through molecular markersAkhilesh Sharma0Devinder Kumar Banyal1Vinod Janardan Dhole2 Bansuli3Ranbir Singh Rana4Rajesh Kumar5Prabhat Kumar6Nimit Kumar7 Srishti8Arshia Prashar9Vivek Singh10Anoushka Sharma11Department of Vegetable Science and Floriculture, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, IndiaDepartment of Plant Pathology, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, IndiaModular Lab, A Block, Nuclear Agriculture and Biotechnology Division (NABTD), Bhabha Atomic Research Centre, Mumbai, IndiaDepartment of Vegetable Science and Floriculture, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, IndiaCenter for Geoinformatics Research and Technology, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, IndiaIndian Council of Agricultural Research, Indian Institute of Vegetable Research, Varanasi, IndiaHorticulture Commissioner, Ministry of Agriculture and Farmers Welfare, Government of India, New Delhi, IndiaDepartment of Genetics and Plant Breeding, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, IndiaDepartment of Vegetable Science and Floriculture, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, IndiaDepartment of Vegetable Science and Floriculture, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, IndiaDepartment of Vegetable Science and Floriculture, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, IndiaDepartment of Genetics and Plant Breeding, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, IndiaPowdery mildew (PM) caused by Erysiphie pisi Syd. is the most devastating disease of pea, affecting fresh pea production as well as the quality of the marketable harvest worldwide. The efforts were made to develop PM-resistant mutants of popular pea varieties “Lincoln” and “Azad P-1” through induced mutations by following gamma irradiation (300, 400, 500, and 600 Gy) and chemical mutagenesis, i.e., ethyl methane sulfonate (EMS) (0.3% and 0.4%). The screening of 13,868 M2 progenies at Kukumseri (summer season) followed by M3 generation at Palampur (winter season) resulted in the isolation of six putative PM-resistant mutants. The rigorous evaluation of these progenies under in vivo (field screening) and in vitro (artificial screening under greenhouse conditions and using the detached leaf assay method) conditions over the years resulted in the isolation of three PM-resistant mutants, viz., L-40-1014, L-0.3-139, and AP-0.3-129. SSR markers “PSMPSAD60 d” and “PSMPA5 c” linked to the er-1 gene indicated the presence of the “er1” gene in the mutant L-0.3-139 while the er-2 gene-linked SCAR marker “ScX171400” and SSR marker “AD141” indicated the probability of the “er-2” gene in mutant L-40-1014. The known markers linked to PM resistance genes could not be validated in the mutant AP-0.3-129, suggested to identify new markers linked to PM resistance. These PM-resistant mutants can be promising candidates as the new source of resistance for future pea breeding programs.https://www.frontiersin.org/articles/10.3389/fpls.2024.1501661/fullmutagenesisgarden peaErysiphe pisi Sydresistancescreeninger genes |
| spellingShingle | Akhilesh Sharma Devinder Kumar Banyal Vinod Janardan Dhole Bansuli Ranbir Singh Rana Rajesh Kumar Prabhat Kumar Nimit Kumar Srishti Arshia Prashar Vivek Singh Anoushka Sharma Development of new powdery mildew resistant lines in garden pea (Pisum sativum L.) using induced mutagenesis and validation of resistance for the er1 and er2 gene through molecular markers Frontiers in Plant Science mutagenesis garden pea Erysiphe pisi Syd resistance screening er genes |
| title | Development of new powdery mildew resistant lines in garden pea (Pisum sativum L.) using induced mutagenesis and validation of resistance for the er1 and er2 gene through molecular markers |
| title_full | Development of new powdery mildew resistant lines in garden pea (Pisum sativum L.) using induced mutagenesis and validation of resistance for the er1 and er2 gene through molecular markers |
| title_fullStr | Development of new powdery mildew resistant lines in garden pea (Pisum sativum L.) using induced mutagenesis and validation of resistance for the er1 and er2 gene through molecular markers |
| title_full_unstemmed | Development of new powdery mildew resistant lines in garden pea (Pisum sativum L.) using induced mutagenesis and validation of resistance for the er1 and er2 gene through molecular markers |
| title_short | Development of new powdery mildew resistant lines in garden pea (Pisum sativum L.) using induced mutagenesis and validation of resistance for the er1 and er2 gene through molecular markers |
| title_sort | development of new powdery mildew resistant lines in garden pea pisum sativum l using induced mutagenesis and validation of resistance for the er1 and er2 gene through molecular markers |
| topic | mutagenesis garden pea Erysiphe pisi Syd resistance screening er genes |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1501661/full |
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