Research Note: Reduction of Salmonella load in Brazilian commercial chicken farms using INSPEKTOR®: a bacteriophage-based product
Salmonella is a zoonotic pathogen transmitted through food and one of the four leading global causes of diarrheal disease in humans. Brazil is the world's largest exporter of poultry meat, and as such, Brazilian farms routinely detect and prevent Salmonella. The last official monitoring and con...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011222 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591858237177856 |
|---|---|
| author | Michael Pino Karen Mujica Paola Mora-Uribe Héctor Garcias-Papayani Braulio Paillavil Camilo Avendaño Diego Flores-Crisosto Rodrigo Norambuena Victoria Rojas-Martínez Matías Aguilera Nicolás Cifuentes Muñoz Pablo Cifuentes Hans Pieringer Soledad Ulloa |
| author_facet | Michael Pino Karen Mujica Paola Mora-Uribe Héctor Garcias-Papayani Braulio Paillavil Camilo Avendaño Diego Flores-Crisosto Rodrigo Norambuena Victoria Rojas-Martínez Matías Aguilera Nicolás Cifuentes Muñoz Pablo Cifuentes Hans Pieringer Soledad Ulloa |
| author_sort | Michael Pino |
| collection | DOAJ |
| description | Salmonella is a zoonotic pathogen transmitted through food and one of the four leading global causes of diarrheal disease in humans. Brazil is the world's largest exporter of poultry meat, and as such, Brazilian farms routinely detect and prevent Salmonella. The last official monitoring and control in poultry slaughter establishments in Brazil in 2023 detected Salmonella spp. in 14.80 % of samples from chickens, and those Salmonella-positive samples were classified as serovar Minnesota in 65.76 % of samples, serovar Heidelberg in 16.10 % of samples and another 26 or more serovars non-typeable represented a total of 18.13 % of the samples. However, despite these measures, several poultry diseases and outbreaks have been reported in recent decades, requiring permanent disease control. Bacteriophages, which act as specific parasites of bacterial cells, can contribute to food safety. This study evaluated the effectiveness of the INSPEKTOR® phage cocktail for controlling Salmonella on 17 commercial broiler farms on a total of 4 experimental groups (1 control cycle and 3 consecutive treatment cycles) using 3 doses of the cocktail for each cycle. Salmonella load was quantified (DNA copies/mL) by real-time PCR using the invA marker for Salmonella spp., together with serovars Minnesota and Heidelberg, which are relevant to the industry in Brazil. Our results showed that INSPEKTOR® reduced Salmonella load from 0.5 up to 6 logs in the treatment cycles 3 and 4 compared to the control cycle, and reduced the load of Minnesota and Heidelberg in all 3 treatment cycles. This study is relevant as it involves applying a phage cocktail capable of reducing Salmonella load in real poultry production systems with over 3 million broiler chickens. |
| format | Article |
| id | doaj-art-9a9c93630bd34abb9d2e3c07cb44c1f1 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-9a9c93630bd34abb9d2e3c07cb44c1f12025-01-22T05:40:25ZengElsevierPoultry Science0032-57912025-01-011041104544Research Note: Reduction of Salmonella load in Brazilian commercial chicken farms using INSPEKTOR®: a bacteriophage-based productMichael Pino0Karen Mujica1Paola Mora-Uribe2Héctor Garcias-Papayani3Braulio Paillavil4Camilo Avendaño5Diego Flores-Crisosto6Rodrigo Norambuena7Victoria Rojas-Martínez8Matías Aguilera9Nicolás Cifuentes Muñoz10Pablo Cifuentes11Hans Pieringer12Soledad Ulloa13PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.PhageLab Chile SpA, Vicuña Mackenna 4860, Centro de Innovación Anacleto Angelini 5th floor, Santiago, Chile.; Corresponding author.Salmonella is a zoonotic pathogen transmitted through food and one of the four leading global causes of diarrheal disease in humans. Brazil is the world's largest exporter of poultry meat, and as such, Brazilian farms routinely detect and prevent Salmonella. The last official monitoring and control in poultry slaughter establishments in Brazil in 2023 detected Salmonella spp. in 14.80 % of samples from chickens, and those Salmonella-positive samples were classified as serovar Minnesota in 65.76 % of samples, serovar Heidelberg in 16.10 % of samples and another 26 or more serovars non-typeable represented a total of 18.13 % of the samples. However, despite these measures, several poultry diseases and outbreaks have been reported in recent decades, requiring permanent disease control. Bacteriophages, which act as specific parasites of bacterial cells, can contribute to food safety. This study evaluated the effectiveness of the INSPEKTOR® phage cocktail for controlling Salmonella on 17 commercial broiler farms on a total of 4 experimental groups (1 control cycle and 3 consecutive treatment cycles) using 3 doses of the cocktail for each cycle. Salmonella load was quantified (DNA copies/mL) by real-time PCR using the invA marker for Salmonella spp., together with serovars Minnesota and Heidelberg, which are relevant to the industry in Brazil. Our results showed that INSPEKTOR® reduced Salmonella load from 0.5 up to 6 logs in the treatment cycles 3 and 4 compared to the control cycle, and reduced the load of Minnesota and Heidelberg in all 3 treatment cycles. This study is relevant as it involves applying a phage cocktail capable of reducing Salmonella load in real poultry production systems with over 3 million broiler chickens.http://www.sciencedirect.com/science/article/pii/S0032579124011222SalmonellaPoultryBacteriophageBroilerINSPEKTOR |
| spellingShingle | Michael Pino Karen Mujica Paola Mora-Uribe Héctor Garcias-Papayani Braulio Paillavil Camilo Avendaño Diego Flores-Crisosto Rodrigo Norambuena Victoria Rojas-Martínez Matías Aguilera Nicolás Cifuentes Muñoz Pablo Cifuentes Hans Pieringer Soledad Ulloa Research Note: Reduction of Salmonella load in Brazilian commercial chicken farms using INSPEKTOR®: a bacteriophage-based product Poultry Science Salmonella Poultry Bacteriophage Broiler INSPEKTOR |
| title | Research Note: Reduction of Salmonella load in Brazilian commercial chicken farms using INSPEKTOR®: a bacteriophage-based product |
| title_full | Research Note: Reduction of Salmonella load in Brazilian commercial chicken farms using INSPEKTOR®: a bacteriophage-based product |
| title_fullStr | Research Note: Reduction of Salmonella load in Brazilian commercial chicken farms using INSPEKTOR®: a bacteriophage-based product |
| title_full_unstemmed | Research Note: Reduction of Salmonella load in Brazilian commercial chicken farms using INSPEKTOR®: a bacteriophage-based product |
| title_short | Research Note: Reduction of Salmonella load in Brazilian commercial chicken farms using INSPEKTOR®: a bacteriophage-based product |
| title_sort | research note reduction of salmonella load in brazilian commercial chicken farms using inspektor r a bacteriophage based product |
| topic | Salmonella Poultry Bacteriophage Broiler INSPEKTOR |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011222 |
| work_keys_str_mv | AT michaelpino researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct AT karenmujica researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct AT paolamorauribe researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct AT hectorgarciaspapayani researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct AT brauliopaillavil researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct AT camiloavendano researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct AT diegoflorescrisosto researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct AT rodrigonorambuena researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct AT victoriarojasmartinez researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct AT matiasaguilera researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct AT nicolascifuentesmunoz researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct AT pablocifuentes researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct AT hanspieringer researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct AT soledadulloa researchnotereductionofsalmonellaloadinbraziliancommercialchickenfarmsusinginspektorabacteriophagebasedproduct |