Characterization of <i>PHT</i> Genes in ‘duli’ (<i>Pyrus betulifolia Bunge</i>) and Expression Analysis of <i>PbPHTs</i> in Response to Plant Growth Regulators, P, and Salt Stress
The phosphate transporter (PHT) family plays an important role in the uptake and transport of P elements in plants. A total of 158 <i>PbPHTs</i> were identified from the genome of ‘duli’ (<i>Pyrus betulifolia Bunge</i>) in this study, including 70 <i>PbPHT1s</i>,...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Agriculture |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0472/15/2/199 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The phosphate transporter (PHT) family plays an important role in the uptake and transport of P elements in plants. A total of 158 <i>PbPHTs</i> were identified from the genome of ‘duli’ (<i>Pyrus betulifolia Bunge</i>) in this study, including 70 <i>PbPHT1s</i>, 2 <i>PbPHT2s</i>, 70 <i>PbPHT3s</i>, 12 <i>PbPHT4s</i>, and 4 <i>PbPHT5s</i>. Among the 158 <i>PHT</i> genes, 150 were localized to 17 ‘duli’ chromosomes. Gene duplication analysis identified 18 tandemly duplicated gene pairs. The promoter analysis showed that there were a large number of cis-acting elements related to phytohormones, growth, development, stress, and light response in <i>PbPHTs</i>. qRT-PCR analysis revealed that most <i>PHT</i> genes in ‘duli’ were highly expressed in the fruits, flowers, leaves, stems, and roots, and 15 <i>PbPHT</i> genes were responsive to 5 μM, 0.5 mM, 5 mM H<sub>2</sub>PO<sub>4</sub>, NaCl, GR24 (synthetic SL analog), GA3 (gibberellin 3), ABA (abscisic acid), and IAA (indole-3-acetic acid). GR24, GA3, IAA, and 5 mM KH<sub>2</sub>PO<sub>4</sub> treatments could increase the concentration, absorption, transport, and distribution of P elements in the rhizomes and leaves of ‘duli’, but 5 μM KH<sub>2</sub>PO<sub>4</sub>, NaCl, and ABA had the opposite effect. This study therefore provides a list of <i>PbPHT</i> genes with substantial roles in abiotic stress response, as well as important information to understand the functional characteristics of <i>PbPHT</i> during ‘duli’ abiotic stress tolerance, and explores the function of PbPHTs in exogenous hormones, phosphorus, and salt stress in the future. |
|---|---|
| ISSN: | 2077-0472 |