Biofilm Formation and Detection of Fluoroquinolone- and Carbapenem-Resistant Genes in Multidrug-Resistant Acinetobacter baumannii
Acinetobacter baumannii is an important opportunistic pathogen that shows resistance to cephalosporins, penicillins, carbapenems, fluoroquinolones, and aminoglycosides, the multiresistance being associated with its ability to form biofilms in clinical environments. The aim of this study was to deter...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2019/3454907 |
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| author | María-Guadalupe Avila-Novoa Oscar-Alberto Solís-Velázquez Daniel-Eduardo Rangel-López Jean-Pierre González-Gómez Pedro-Javier Guerrero-Medina Melesio Gutiérrez-Lomelí |
| author_facet | María-Guadalupe Avila-Novoa Oscar-Alberto Solís-Velázquez Daniel-Eduardo Rangel-López Jean-Pierre González-Gómez Pedro-Javier Guerrero-Medina Melesio Gutiérrez-Lomelí |
| author_sort | María-Guadalupe Avila-Novoa |
| collection | DOAJ |
| description | Acinetobacter baumannii is an important opportunistic pathogen that shows resistance to cephalosporins, penicillins, carbapenems, fluoroquinolones, and aminoglycosides, the multiresistance being associated with its ability to form biofilms in clinical environments. The aim of this study was to determine biofilm formation and its potential association with genes involved in antibiotic resistance mechanisms of A. baumannii isolates of different clinical specimens. We demonstrated 100% of the A. baumannii isolates examined to be multidrug resistant (MDR), presenting a 73.3% susceptibility to cefepime and a 53.3% susceptibility to ciprofloxacin. All A. baumannii isolates were positive for blaOXA-51, 33.3% being positive for blaOXA-23 and ISAba1, and 73.3% being positive for gyrA. We found 86.6% of A. baumannii strains to be low-grade biofilm formers and 13.3% to be biofilm negative; culturing on Congo red agar (CRA) plates revealed that 73.3% of the A. baumannii isolates to be biofilm producers, while 26.6% were not. These properties, combined with the role of A. baumannii as a nosocomial pathogen, increase the probability of A. baumannii causing nosocomial infections and outbreaks as a complication during therapeutic treatments and emphasize the need to control A. baumannii biofilms in hospital environments. |
| format | Article |
| id | doaj-art-9a23dbe0ca384f93ab83b31426c0c59d |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-9a23dbe0ca384f93ab83b31426c0c59d2025-02-03T05:45:54ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932019-01-01201910.1155/2019/34549073454907Biofilm Formation and Detection of Fluoroquinolone- and Carbapenem-Resistant Genes in Multidrug-Resistant Acinetobacter baumanniiMaría-Guadalupe Avila-Novoa0Oscar-Alberto Solís-Velázquez1Daniel-Eduardo Rangel-López2Jean-Pierre González-Gómez3Pedro-Javier Guerrero-Medina4Melesio Gutiérrez-Lomelí5Laboratorio de Alimentos, Departamento de Ciencias Médicas y de la Vida, División de Desarrollo Biotecnológico, Universidad de Guadalajara, Centro Universitario de la Ciénega. Av. Universidad 1115, Col. Linda Vista, 47820 Ocotlán, Jalisco, MexicoLaboratorio de Alimentos, Departamento de Ciencias Médicas y de la Vida, División de Desarrollo Biotecnológico, Universidad de Guadalajara, Centro Universitario de la Ciénega. Av. Universidad 1115, Col. Linda Vista, 47820 Ocotlán, Jalisco, MexicoLaboratorio de Alimentos, Departamento de Ciencias Médicas y de la Vida, División de Desarrollo Biotecnológico, Universidad de Guadalajara, Centro Universitario de la Ciénega. Av. Universidad 1115, Col. Linda Vista, 47820 Ocotlán, Jalisco, MexicoLaboratorio de Alimentos, Departamento de Ciencias Médicas y de la Vida, División de Desarrollo Biotecnológico, Universidad de Guadalajara, Centro Universitario de la Ciénega. Av. Universidad 1115, Col. Linda Vista, 47820 Ocotlán, Jalisco, MexicoLaboratorio de Alimentos, Departamento de Ciencias Médicas y de la Vida, División de Desarrollo Biotecnológico, Universidad de Guadalajara, Centro Universitario de la Ciénega. Av. Universidad 1115, Col. Linda Vista, 47820 Ocotlán, Jalisco, MexicoLaboratorio de Alimentos, Departamento de Ciencias Médicas y de la Vida, División de Desarrollo Biotecnológico, Universidad de Guadalajara, Centro Universitario de la Ciénega. Av. Universidad 1115, Col. Linda Vista, 47820 Ocotlán, Jalisco, MexicoAcinetobacter baumannii is an important opportunistic pathogen that shows resistance to cephalosporins, penicillins, carbapenems, fluoroquinolones, and aminoglycosides, the multiresistance being associated with its ability to form biofilms in clinical environments. The aim of this study was to determine biofilm formation and its potential association with genes involved in antibiotic resistance mechanisms of A. baumannii isolates of different clinical specimens. We demonstrated 100% of the A. baumannii isolates examined to be multidrug resistant (MDR), presenting a 73.3% susceptibility to cefepime and a 53.3% susceptibility to ciprofloxacin. All A. baumannii isolates were positive for blaOXA-51, 33.3% being positive for blaOXA-23 and ISAba1, and 73.3% being positive for gyrA. We found 86.6% of A. baumannii strains to be low-grade biofilm formers and 13.3% to be biofilm negative; culturing on Congo red agar (CRA) plates revealed that 73.3% of the A. baumannii isolates to be biofilm producers, while 26.6% were not. These properties, combined with the role of A. baumannii as a nosocomial pathogen, increase the probability of A. baumannii causing nosocomial infections and outbreaks as a complication during therapeutic treatments and emphasize the need to control A. baumannii biofilms in hospital environments.http://dx.doi.org/10.1155/2019/3454907 |
| spellingShingle | María-Guadalupe Avila-Novoa Oscar-Alberto Solís-Velázquez Daniel-Eduardo Rangel-López Jean-Pierre González-Gómez Pedro-Javier Guerrero-Medina Melesio Gutiérrez-Lomelí Biofilm Formation and Detection of Fluoroquinolone- and Carbapenem-Resistant Genes in Multidrug-Resistant Acinetobacter baumannii Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Biofilm Formation and Detection of Fluoroquinolone- and Carbapenem-Resistant Genes in Multidrug-Resistant Acinetobacter baumannii |
| title_full | Biofilm Formation and Detection of Fluoroquinolone- and Carbapenem-Resistant Genes in Multidrug-Resistant Acinetobacter baumannii |
| title_fullStr | Biofilm Formation and Detection of Fluoroquinolone- and Carbapenem-Resistant Genes in Multidrug-Resistant Acinetobacter baumannii |
| title_full_unstemmed | Biofilm Formation and Detection of Fluoroquinolone- and Carbapenem-Resistant Genes in Multidrug-Resistant Acinetobacter baumannii |
| title_short | Biofilm Formation and Detection of Fluoroquinolone- and Carbapenem-Resistant Genes in Multidrug-Resistant Acinetobacter baumannii |
| title_sort | biofilm formation and detection of fluoroquinolone and carbapenem resistant genes in multidrug resistant acinetobacter baumannii |
| url | http://dx.doi.org/10.1155/2019/3454907 |
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