6‐Nitrodopamine potentiates catecholamine‐induced contractions of human isolated vas deferens
Abstract 6‐Nitrodopamine (6‐ND) is the main catecholamine released from human isolated vas deferens and the adrenergic nervous system is known to play a major role in the contractions of the epididymal portion of the vas deferens. Here it was investigated the interactions of 6‐ND on the contractions...
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| Format: | Article |
| Language: | English |
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Wiley
2025-02-01
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| Series: | FASEB BioAdvances |
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| Online Access: | https://doi.org/10.1096/fba.2024-00183 |
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| author | Antonio Tiago Lima Sami Jabbour José Britto‐Júnior Demétrio Martinho Ramos deCarvalho Adriano Fregonesi Fernanda V. Mariano Valéria Barbosa deSouza Andre Almeida Schenka Edson Antunes Gilberto De Nucci |
| author_facet | Antonio Tiago Lima Sami Jabbour José Britto‐Júnior Demétrio Martinho Ramos deCarvalho Adriano Fregonesi Fernanda V. Mariano Valéria Barbosa deSouza Andre Almeida Schenka Edson Antunes Gilberto De Nucci |
| author_sort | Antonio Tiago Lima |
| collection | DOAJ |
| description | Abstract 6‐Nitrodopamine (6‐ND) is the main catecholamine released from human isolated vas deferens and the adrenergic nervous system is known to play a major role in the contractions of the epididymal portion of the vas deferens. Here it was investigated the interactions of 6‐ND on the contractions of the human isolated vas deferens induced by either classical catecholamines or electric‐field stimulation (EFS). The vas deferens obtained from 106 patients who underwent vasectomy surgery were mounted in a 10‐mL glass chamber filled with warmed (37°C) and oxygenated Krebs–Henseleit's solution. The strips were pretreated (30 min) with 6‐ND (0.1–100 nM) and exposed to increasing concentrations of noradrenaline (0.01–300 M), dopamine (0.00001–10 mM), or adrenaline (0.01–300 M). The strips were also submitted to EFS in tissues pre‐incubated or not with 6‐ND (1–100 nM), noradrenaline (100 nM), adrenaline (100 nM), or dopamine (100 nM). Catecholamine basal release was evaluated by LC–MS/MS and expression of tyrosine hydroxylase by both immunohistochemistry (IC) and fluorescence in‐situ hybridization (FISH). Pre‐incubation of the vas deferens with 6‐ND caused marked potentiation of the contractions induced by noradrenaline, adrenaline, and dopamine, as characterized by significant increases in Emax, without changes in pEC50 values. 6‐nitrodopamine also caused significant increases in the EFS‐induced contractions. The basal release of 6‐ND was not affected by pre‐treatment of the tissues with tetrodotoxin. Tyrosine hydroxylase was detected in epithelial cells of human vas deferens samples by both IC and FISH. The results clearly demonstrate that epithelium‐derived 6‐ND is a major modulator of human vas deferens contractility. |
| format | Article |
| id | doaj-art-9a066544687240a8afbb122303a281b9 |
| institution | Kabale University |
| issn | 2573-9832 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley |
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| series | FASEB BioAdvances |
| spelling | doaj-art-9a066544687240a8afbb122303a281b92025-02-05T11:30:19ZengWileyFASEB BioAdvances2573-98322025-02-0172n/an/a10.1096/fba.2024-001836‐Nitrodopamine potentiates catecholamine‐induced contractions of human isolated vas deferensAntonio Tiago Lima0Sami Jabbour1José Britto‐Júnior2Demétrio Martinho Ramos deCarvalho3Adriano Fregonesi4Fernanda V. Mariano5Valéria Barbosa deSouza6Andre Almeida Schenka7Edson Antunes8Gilberto De Nucci9Department of Pharmacology, Faculty of Medical Sciences State University of Campinas (UNICAMP) Campinas BrazilDepartment of Surgery, Faculty of Medicine of Jundiaí Jundiaí BrazilDepartment of Pharmacology, Faculty of Medical Sciences State University of Campinas (UNICAMP) Campinas BrazilMetropolitan University of Santos (UNIMES) Santos BrazilDepartment of Surgery, Faculty of Medicine of Jundiaí Jundiaí BrazilDepartment of Pathology, Faculty of Medical Sciences State University of Campinas (UNICAMP) Campinas BrazilDepartment of Pharmacology, Faculty of Medical Sciences State University of Campinas (UNICAMP) Campinas BrazilDepartment of Pharmacology, Faculty of Medical Sciences State University of Campinas (UNICAMP) Campinas BrazilDepartment of Pharmacology, Faculty of Medical Sciences State University of Campinas (UNICAMP) Campinas BrazilDepartment of Pharmacology, Faculty of Medical Sciences State University of Campinas (UNICAMP) Campinas BrazilAbstract 6‐Nitrodopamine (6‐ND) is the main catecholamine released from human isolated vas deferens and the adrenergic nervous system is known to play a major role in the contractions of the epididymal portion of the vas deferens. Here it was investigated the interactions of 6‐ND on the contractions of the human isolated vas deferens induced by either classical catecholamines or electric‐field stimulation (EFS). The vas deferens obtained from 106 patients who underwent vasectomy surgery were mounted in a 10‐mL glass chamber filled with warmed (37°C) and oxygenated Krebs–Henseleit's solution. The strips were pretreated (30 min) with 6‐ND (0.1–100 nM) and exposed to increasing concentrations of noradrenaline (0.01–300 M), dopamine (0.00001–10 mM), or adrenaline (0.01–300 M). The strips were also submitted to EFS in tissues pre‐incubated or not with 6‐ND (1–100 nM), noradrenaline (100 nM), adrenaline (100 nM), or dopamine (100 nM). Catecholamine basal release was evaluated by LC–MS/MS and expression of tyrosine hydroxylase by both immunohistochemistry (IC) and fluorescence in‐situ hybridization (FISH). Pre‐incubation of the vas deferens with 6‐ND caused marked potentiation of the contractions induced by noradrenaline, adrenaline, and dopamine, as characterized by significant increases in Emax, without changes in pEC50 values. 6‐nitrodopamine also caused significant increases in the EFS‐induced contractions. The basal release of 6‐ND was not affected by pre‐treatment of the tissues with tetrodotoxin. Tyrosine hydroxylase was detected in epithelial cells of human vas deferens samples by both IC and FISH. The results clearly demonstrate that epithelium‐derived 6‐ND is a major modulator of human vas deferens contractility.https://doi.org/10.1096/fba.2024-00183epitheliumliquid chromatography coupled to mass spectrometrynitrocatecholaminessynergism |
| spellingShingle | Antonio Tiago Lima Sami Jabbour José Britto‐Júnior Demétrio Martinho Ramos deCarvalho Adriano Fregonesi Fernanda V. Mariano Valéria Barbosa deSouza Andre Almeida Schenka Edson Antunes Gilberto De Nucci 6‐Nitrodopamine potentiates catecholamine‐induced contractions of human isolated vas deferens FASEB BioAdvances epithelium liquid chromatography coupled to mass spectrometry nitrocatecholamines synergism |
| title | 6‐Nitrodopamine potentiates catecholamine‐induced contractions of human isolated vas deferens |
| title_full | 6‐Nitrodopamine potentiates catecholamine‐induced contractions of human isolated vas deferens |
| title_fullStr | 6‐Nitrodopamine potentiates catecholamine‐induced contractions of human isolated vas deferens |
| title_full_unstemmed | 6‐Nitrodopamine potentiates catecholamine‐induced contractions of human isolated vas deferens |
| title_short | 6‐Nitrodopamine potentiates catecholamine‐induced contractions of human isolated vas deferens |
| title_sort | 6 nitrodopamine potentiates catecholamine induced contractions of human isolated vas deferens |
| topic | epithelium liquid chromatography coupled to mass spectrometry nitrocatecholamines synergism |
| url | https://doi.org/10.1096/fba.2024-00183 |
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