Alginate-based encapsulation of porcine placenta extract: Preparation, enteric sustained release, biological activities, and stability
The porcine placenta is a source of various advantageous bioactive molecules, however, the unsatisfactory appearance, together with its unsavory taste and odor, is still limiting its use as an ingredient in food products. Microencapsulation represents a promising technique to increase the use of val...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | Food Hydrocolloids for Health |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667025924000190 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832583038808096768 |
|---|---|
| author | Anukul Taweechaipaisankul Nutthanit Thumrongsiri Walailuk Chonniyom Paweena Dana Prattana Tanyapanyachon Monthira Rattanatayarom Wannapa Chinchoosak Nattika Saengkrit |
| author_facet | Anukul Taweechaipaisankul Nutthanit Thumrongsiri Walailuk Chonniyom Paweena Dana Prattana Tanyapanyachon Monthira Rattanatayarom Wannapa Chinchoosak Nattika Saengkrit |
| author_sort | Anukul Taweechaipaisankul |
| collection | DOAJ |
| description | The porcine placenta is a source of various advantageous bioactive molecules, however, the unsatisfactory appearance, together with its unsavory taste and odor, is still limiting its use as an ingredient in food products. Microencapsulation represents a promising technique to increase the use of valuable biowaste, as well as to promote its bioavailability and stability. Here, alginate microbeads containing porcine placenta extracts (pPEs) were developed by the ionotropic gelation method under various conditions, and many of their properties were tested. We found that placenta tissues prepared by freeze-drying could provide a great yield of protein derivatives and possessed 50.37 % anti-oxidative activity. Formulation 2 (F2) of alginate microbeads (80 % solution containing 2 % sodium alginate with 1 % bentonite, 20 % pPE formed in 0.5 % chitosan added into 1 % CaCl2 solution) was chosen as the optimal encapsulated condition of pPEs. Generally, the F2 microbeads contained 57.5 µg/mL of pPE with 99.72 % entrapment efficiency. The average size measured by Mastersizer was 14.65 ± 0.08 µm. The release of pPEs under simulated gastrointestinal tract conditions at pH 2.0–6.8 was delayed at 2 h (38.7 %) compared with free pPE. The F2 microbeads showed positive biological effects of anti-oxidation. Moreover, the anti-inflammation effect was monitored via the reduction in the levels of cytokines, including IL-6, IL-8, and TNF-α. The F2 microbeads maintained their protein quantities for >120 days at 25 °C. Taken together, microbead fabrication, especially F2, is the optimal formula for pPEs, showing the potential to be applied as a prospective carrier of pPEs for oral administration. |
| format | Article |
| id | doaj-art-99ca63a2854c4d7ca0aab4f27c5ffc75 |
| institution | Kabale University |
| issn | 2667-0259 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Hydrocolloids for Health |
| spelling | doaj-art-99ca63a2854c4d7ca0aab4f27c5ffc752025-01-29T05:02:20ZengElsevierFood Hydrocolloids for Health2667-02592025-06-017100194Alginate-based encapsulation of porcine placenta extract: Preparation, enteric sustained release, biological activities, and stabilityAnukul Taweechaipaisankul0Nutthanit Thumrongsiri1Walailuk Chonniyom2Paweena Dana3Prattana Tanyapanyachon4Monthira Rattanatayarom5Wannapa Chinchoosak6Nattika Saengkrit7National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand; Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, ThailandSaraburi Farm, Saraburi 18000, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand; Correspondence author at: National Nanotechnology Center, National Science and Technology Development Agency, Pathumthani, 12120, Thailand.The porcine placenta is a source of various advantageous bioactive molecules, however, the unsatisfactory appearance, together with its unsavory taste and odor, is still limiting its use as an ingredient in food products. Microencapsulation represents a promising technique to increase the use of valuable biowaste, as well as to promote its bioavailability and stability. Here, alginate microbeads containing porcine placenta extracts (pPEs) were developed by the ionotropic gelation method under various conditions, and many of their properties were tested. We found that placenta tissues prepared by freeze-drying could provide a great yield of protein derivatives and possessed 50.37 % anti-oxidative activity. Formulation 2 (F2) of alginate microbeads (80 % solution containing 2 % sodium alginate with 1 % bentonite, 20 % pPE formed in 0.5 % chitosan added into 1 % CaCl2 solution) was chosen as the optimal encapsulated condition of pPEs. Generally, the F2 microbeads contained 57.5 µg/mL of pPE with 99.72 % entrapment efficiency. The average size measured by Mastersizer was 14.65 ± 0.08 µm. The release of pPEs under simulated gastrointestinal tract conditions at pH 2.0–6.8 was delayed at 2 h (38.7 %) compared with free pPE. The F2 microbeads showed positive biological effects of anti-oxidation. Moreover, the anti-inflammation effect was monitored via the reduction in the levels of cytokines, including IL-6, IL-8, and TNF-α. The F2 microbeads maintained their protein quantities for >120 days at 25 °C. Taken together, microbead fabrication, especially F2, is the optimal formula for pPEs, showing the potential to be applied as a prospective carrier of pPEs for oral administration.http://www.sciencedirect.com/science/article/pii/S2667025924000190Alginate microbeadsEnteric controlled releaseIonotropic gelationMicroencapsulationPorcine placenta extracts |
| spellingShingle | Anukul Taweechaipaisankul Nutthanit Thumrongsiri Walailuk Chonniyom Paweena Dana Prattana Tanyapanyachon Monthira Rattanatayarom Wannapa Chinchoosak Nattika Saengkrit Alginate-based encapsulation of porcine placenta extract: Preparation, enteric sustained release, biological activities, and stability Food Hydrocolloids for Health Alginate microbeads Enteric controlled release Ionotropic gelation Microencapsulation Porcine placenta extracts |
| title | Alginate-based encapsulation of porcine placenta extract: Preparation, enteric sustained release, biological activities, and stability |
| title_full | Alginate-based encapsulation of porcine placenta extract: Preparation, enteric sustained release, biological activities, and stability |
| title_fullStr | Alginate-based encapsulation of porcine placenta extract: Preparation, enteric sustained release, biological activities, and stability |
| title_full_unstemmed | Alginate-based encapsulation of porcine placenta extract: Preparation, enteric sustained release, biological activities, and stability |
| title_short | Alginate-based encapsulation of porcine placenta extract: Preparation, enteric sustained release, biological activities, and stability |
| title_sort | alginate based encapsulation of porcine placenta extract preparation enteric sustained release biological activities and stability |
| topic | Alginate microbeads Enteric controlled release Ionotropic gelation Microencapsulation Porcine placenta extracts |
| url | http://www.sciencedirect.com/science/article/pii/S2667025924000190 |
| work_keys_str_mv | AT anukultaweechaipaisankul alginatebasedencapsulationofporcineplacentaextractpreparationentericsustainedreleasebiologicalactivitiesandstability AT nutthanitthumrongsiri alginatebasedencapsulationofporcineplacentaextractpreparationentericsustainedreleasebiologicalactivitiesandstability AT walailukchonniyom alginatebasedencapsulationofporcineplacentaextractpreparationentericsustainedreleasebiologicalactivitiesandstability AT paweenadana alginatebasedencapsulationofporcineplacentaextractpreparationentericsustainedreleasebiologicalactivitiesandstability AT prattanatanyapanyachon alginatebasedencapsulationofporcineplacentaextractpreparationentericsustainedreleasebiologicalactivitiesandstability AT monthirarattanatayarom alginatebasedencapsulationofporcineplacentaextractpreparationentericsustainedreleasebiologicalactivitiesandstability AT wannapachinchoosak alginatebasedencapsulationofporcineplacentaextractpreparationentericsustainedreleasebiologicalactivitiesandstability AT nattikasaengkrit alginatebasedencapsulationofporcineplacentaextractpreparationentericsustainedreleasebiologicalactivitiesandstability |