Evidence of the Complexity of Gene Expression Analysis in Fish Wild Populations
The present work examines the induction of the band 3 anion transport protein, mitogen-activated protein kinase, and lactate dehydrogenase, respectively related to osmolyte transport, cell volume regulation, and energy production in the gills of two tilapia strains exposed to either freshwater or hy...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/1258396 |
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| _version_ | 1832564135775174656 |
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| author | Mbaye Tine |
| author_facet | Mbaye Tine |
| author_sort | Mbaye Tine |
| collection | DOAJ |
| description | The present work examines the induction of the band 3 anion transport protein, mitogen-activated protein kinase, and lactate dehydrogenase, respectively related to osmolyte transport, cell volume regulation, and energy production in the gills of two tilapia strains exposed to either freshwater or hypersaline water. Overall, genes showed similar expression patterns between strains. However, a wild population survey across a range of natural habitats and salinities did not reveal the expected patterns. Although significant, the correlations between gene expression and salinity were slightly ambiguous and did not show any link with phenotypic differences in life history traits previously reported between the same populations. The differential expression was also not associated with the population genetic structure inferred from neutral markers. The results suggest that the differential expression observed is not the result of evolutionary forces such as genetic drift or adaptation by natural selection. Instead, it can be speculated that genes responded to various abiotic and biotic stressors, including factors intrinsic to animals. This study provides clear evidence of the complexity of gene expression analysis in wild populations and shows that more attention needs to be paid when selecting candidates as potential biomarkers for monitoring adaptive responses to a specific environmental perturbation. |
| format | Article |
| id | doaj-art-991068a80b9b478eabfe9f6c6fb83f5f |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-991068a80b9b478eabfe9f6c6fb83f5f2025-02-03T01:11:40ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/12583961258396Evidence of the Complexity of Gene Expression Analysis in Fish Wild PopulationsMbaye Tine0UFR des Sciences Agronomiques, de l’Aquaculture et des Technologies Alimentaires (UFR S2ATA), Universite Gaston Berger (UGB), Route de Ngallele BP 234, Saint-Louis, SenegalThe present work examines the induction of the band 3 anion transport protein, mitogen-activated protein kinase, and lactate dehydrogenase, respectively related to osmolyte transport, cell volume regulation, and energy production in the gills of two tilapia strains exposed to either freshwater or hypersaline water. Overall, genes showed similar expression patterns between strains. However, a wild population survey across a range of natural habitats and salinities did not reveal the expected patterns. Although significant, the correlations between gene expression and salinity were slightly ambiguous and did not show any link with phenotypic differences in life history traits previously reported between the same populations. The differential expression was also not associated with the population genetic structure inferred from neutral markers. The results suggest that the differential expression observed is not the result of evolutionary forces such as genetic drift or adaptation by natural selection. Instead, it can be speculated that genes responded to various abiotic and biotic stressors, including factors intrinsic to animals. This study provides clear evidence of the complexity of gene expression analysis in wild populations and shows that more attention needs to be paid when selecting candidates as potential biomarkers for monitoring adaptive responses to a specific environmental perturbation.http://dx.doi.org/10.1155/2017/1258396 |
| spellingShingle | Mbaye Tine Evidence of the Complexity of Gene Expression Analysis in Fish Wild Populations International Journal of Genomics |
| title | Evidence of the Complexity of Gene Expression Analysis in Fish Wild Populations |
| title_full | Evidence of the Complexity of Gene Expression Analysis in Fish Wild Populations |
| title_fullStr | Evidence of the Complexity of Gene Expression Analysis in Fish Wild Populations |
| title_full_unstemmed | Evidence of the Complexity of Gene Expression Analysis in Fish Wild Populations |
| title_short | Evidence of the Complexity of Gene Expression Analysis in Fish Wild Populations |
| title_sort | evidence of the complexity of gene expression analysis in fish wild populations |
| url | http://dx.doi.org/10.1155/2017/1258396 |
| work_keys_str_mv | AT mbayetine evidenceofthecomplexityofgeneexpressionanalysisinfishwildpopulations |