Evaluation of the Oxidative Effect of Long-Term Repetitive Hyperbaric Oxygen Exposures on Different Brain Regions of Rats
Hyperbaric oxygen (HBO2) exposure affects both oxidative and antioxidant systems. This effect is positively correlated with the exposure time and duration of the treatment. The present study aims enlightening the relation of HBO2 with oxidative/antioxidant systems when administered in a prolonged an...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/849183 |
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| author | Kemal Simsek Mehmet Ozler Ali Osman Yildirim Serdar Sadir Seref Demirbas Muzaffer Oztosun Ahmet Korkmaz Hakan Ay Sukru Oter Senol Yildiz |
| author_facet | Kemal Simsek Mehmet Ozler Ali Osman Yildirim Serdar Sadir Seref Demirbas Muzaffer Oztosun Ahmet Korkmaz Hakan Ay Sukru Oter Senol Yildiz |
| author_sort | Kemal Simsek |
| collection | DOAJ |
| description | Hyperbaric oxygen (HBO2) exposure affects both oxidative and antioxidant systems. This effect is positively correlated with the exposure time and duration of the treatment. The present study aims enlightening the relation of HBO2 with oxidative/antioxidant systems when administered in a prolonged and repetitive manner in brain tissues of rats. Sixty rats were divided into 6 study (n=8 for each) and 1 control (n=12) group. Rats in the study groups were daily exposed 90-min HBO2 sessions at 2.8 ATA for 5, 10, 15, 20, 30 and 40 days. One day after the last session, animals were sacrificed; their whole brain tissue was harvested and dissected into three different regions as the outer grey matter (cortex), the inner white matter and cerebellum. Levels of lipid peroxidation and protein oxidation and activities of superoxide dismutase and glutathione peroxidase were measured in these tissues. Malondialdehyde, carbonylated protein and glutathione peroxidase levels were found to be insignificantly increased at different time-points in the cerebral cortex, inner white matter and cerebellum, respectively. These comparable results provide evidence for the safety of HBO treatments and/or successful adaptive mechanisms at least in the brain tissue of rats, even when administered for longer periods. |
| format | Article |
| id | doaj-art-98c5152c5a26433e925f32c44bb5e46e |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-98c5152c5a26433e925f32c44bb5e46e2025-02-03T01:28:51ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/849183849183Evaluation of the Oxidative Effect of Long-Term Repetitive Hyperbaric Oxygen Exposures on Different Brain Regions of RatsKemal Simsek0Mehmet Ozler1Ali Osman Yildirim2Serdar Sadir3Seref Demirbas4Muzaffer Oztosun5Ahmet Korkmaz6Hakan Ay7Sukru Oter8Senol Yildiz9Department of Undersea and Hyperbaric Medicine, Gulhane Military Medical Academy, 06010 Etlik, Ankara, TurkeyDepartment of Physiology, Gulhane Military Medical Academy, 06010 Etlik, Ankara, TurkeyDepartment of Emergency Medicine, Gulhane Military Medical Academy, 06010 Etlik, Ankara, TurkeyDepartment of Emergency Medicine, Gulhane Military Medical Academy, 06010 Etlik, Ankara, TurkeyDepartment of Internal Medicine, Gulhane Military Medical Academy, 06010 Etlik, Ankara, TurkeyHealth Service Command, Turkish Armed Forces, 06790 Etimesgut, Ankara, TurkeyDepartment of Physiology, Gulhane Military Medical Academy, 06010 Etlik, Ankara, TurkeyDepartment of Undersea and Hyperbaric Medicine, Gulhane Military Medical Academy, 06010 Etlik, Ankara, TurkeyDepartment of Physiology, Gulhane Military Medical Academy, 06010 Etlik, Ankara, TurkeyDepartment of Undersea and Hyperbaric Medicine, Gulhane Military Medical Academy, 06010 Etlik, Ankara, TurkeyHyperbaric oxygen (HBO2) exposure affects both oxidative and antioxidant systems. This effect is positively correlated with the exposure time and duration of the treatment. The present study aims enlightening the relation of HBO2 with oxidative/antioxidant systems when administered in a prolonged and repetitive manner in brain tissues of rats. Sixty rats were divided into 6 study (n=8 for each) and 1 control (n=12) group. Rats in the study groups were daily exposed 90-min HBO2 sessions at 2.8 ATA for 5, 10, 15, 20, 30 and 40 days. One day after the last session, animals were sacrificed; their whole brain tissue was harvested and dissected into three different regions as the outer grey matter (cortex), the inner white matter and cerebellum. Levels of lipid peroxidation and protein oxidation and activities of superoxide dismutase and glutathione peroxidase were measured in these tissues. Malondialdehyde, carbonylated protein and glutathione peroxidase levels were found to be insignificantly increased at different time-points in the cerebral cortex, inner white matter and cerebellum, respectively. These comparable results provide evidence for the safety of HBO treatments and/or successful adaptive mechanisms at least in the brain tissue of rats, even when administered for longer periods.http://dx.doi.org/10.1100/2012/849183 |
| spellingShingle | Kemal Simsek Mehmet Ozler Ali Osman Yildirim Serdar Sadir Seref Demirbas Muzaffer Oztosun Ahmet Korkmaz Hakan Ay Sukru Oter Senol Yildiz Evaluation of the Oxidative Effect of Long-Term Repetitive Hyperbaric Oxygen Exposures on Different Brain Regions of Rats The Scientific World Journal |
| title | Evaluation of the Oxidative Effect of Long-Term Repetitive Hyperbaric Oxygen Exposures on Different Brain Regions of Rats |
| title_full | Evaluation of the Oxidative Effect of Long-Term Repetitive Hyperbaric Oxygen Exposures on Different Brain Regions of Rats |
| title_fullStr | Evaluation of the Oxidative Effect of Long-Term Repetitive Hyperbaric Oxygen Exposures on Different Brain Regions of Rats |
| title_full_unstemmed | Evaluation of the Oxidative Effect of Long-Term Repetitive Hyperbaric Oxygen Exposures on Different Brain Regions of Rats |
| title_short | Evaluation of the Oxidative Effect of Long-Term Repetitive Hyperbaric Oxygen Exposures on Different Brain Regions of Rats |
| title_sort | evaluation of the oxidative effect of long term repetitive hyperbaric oxygen exposures on different brain regions of rats |
| url | http://dx.doi.org/10.1100/2012/849183 |
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