Evaluating the Toxicity and Histological Effects of Al2O3 Nanoparticles on Bone Tissue in Animal Model: A Case-Control Study
The applications of nanostructures have been limited by their different toxicities. So, the investigation of these toxicities is necessary before nanostructure application. This study aimed to evaluate the effect of aluminum oxide (Al2O3) nanoparticles on bone density in Wistar rat. Al2O3 nanopartic...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2020/8870530 |
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| author | Hossein Soltaninejad Hadi Zare-Zardini Amir Ali Hamidieh Mohammad Reza Sobhan Seyed Houssein Saeed-Banadaky Mohammad Amir Amirkhani Behnaz Tolueinia Mohsen Mehregan Mahnaz Mirakhor Farzaneh sadat Eshaghi |
| author_facet | Hossein Soltaninejad Hadi Zare-Zardini Amir Ali Hamidieh Mohammad Reza Sobhan Seyed Houssein Saeed-Banadaky Mohammad Amir Amirkhani Behnaz Tolueinia Mohsen Mehregan Mahnaz Mirakhor Farzaneh sadat Eshaghi |
| author_sort | Hossein Soltaninejad |
| collection | DOAJ |
| description | The applications of nanostructures have been limited by their different toxicities. So, the investigation of these toxicities is necessary before nanostructure application. This study aimed to evaluate the effect of aluminum oxide (Al2O3) nanoparticles on bone density in Wistar rat. Al2O3 nanoparticle was prepared by the sol-gel method. Characterization was done by X-ray diffraction (XRD) and transmission electron microscopy (TEM). Sixty-four male adult Wistar rats were divided into eight groups including six groups intravenously treated with Al2O3 nanoparticle at concentrations of 25, 50, 100, 250, 500, and 1000 µg/ml: one group received food and water as the control group, and one group received food and water as well as intravenously distilled water as an injection control group. After 41 days, bone density was analyzed by dual-energy X-ray absorptiometry (DEXA). According to X-ray diffraction, the average particle size for Al2O3 nanoparticles was 20.85 nm. The data of densitometry showed that the bone density of right and left foot was reduced in concentrations of 250, 500, and 1000 µg/ml that were statistically significant in comparison with the control group. The reduction of bone density was increased with the enhancement of nanostructures concentration. The effect of Al2O3 nanoparticles on bone density was similar in the left and right legs. Histopatholological assessment also showed that Al2O3 nanoparticles (250, 500, and 1000 µg/ml) lead to significant reduction of trabeculae. Empty lacunae are observed in these three groups. Considering that high concentrations of Al2O3 nanoparticles had toxicity on bone tissue, it must be used by more caution, especially its use as a coating in different devices such as implants, surgical instruments, and bone prostheses. |
| format | Article |
| id | doaj-art-97866f46760a4b279326b8d6e519ed5a |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-97866f46760a4b279326b8d6e519ed5a2025-02-03T06:05:35ZengWileyJournal of Toxicology1687-81911687-82052020-01-01202010.1155/2020/88705308870530Evaluating the Toxicity and Histological Effects of Al2O3 Nanoparticles on Bone Tissue in Animal Model: A Case-Control StudyHossein Soltaninejad0Hadi Zare-Zardini1Amir Ali Hamidieh2Mohammad Reza Sobhan3Seyed Houssein Saeed-Banadaky4Mohammad Amir Amirkhani5Behnaz Tolueinia6Mohsen Mehregan7Mahnaz Mirakhor8Farzaneh sadat Eshaghi9Department of Nano Biotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran 14115, IranHematology and Oncology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IranIranian Tissue Bank & Research Center, Tehran University of Medical Sciences, Tehran, IranDepartment of Orthopedics, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Orthopedics, Shahid Sadoughi University of Medical Sciences, Yazd, IranIranian Tissue Bank & Research Center, Tehran University of Medical Sciences, Tehran, IranUniversity of Applied Science and Technology of Sistan and Baluchestan, Minushargh Branch, Zahedan, IranDepartment of Physics, Payam-e-noor University, Zahedan, IranShahid Sadoughi Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranThe applications of nanostructures have been limited by their different toxicities. So, the investigation of these toxicities is necessary before nanostructure application. This study aimed to evaluate the effect of aluminum oxide (Al2O3) nanoparticles on bone density in Wistar rat. Al2O3 nanoparticle was prepared by the sol-gel method. Characterization was done by X-ray diffraction (XRD) and transmission electron microscopy (TEM). Sixty-four male adult Wistar rats were divided into eight groups including six groups intravenously treated with Al2O3 nanoparticle at concentrations of 25, 50, 100, 250, 500, and 1000 µg/ml: one group received food and water as the control group, and one group received food and water as well as intravenously distilled water as an injection control group. After 41 days, bone density was analyzed by dual-energy X-ray absorptiometry (DEXA). According to X-ray diffraction, the average particle size for Al2O3 nanoparticles was 20.85 nm. The data of densitometry showed that the bone density of right and left foot was reduced in concentrations of 250, 500, and 1000 µg/ml that were statistically significant in comparison with the control group. The reduction of bone density was increased with the enhancement of nanostructures concentration. The effect of Al2O3 nanoparticles on bone density was similar in the left and right legs. Histopatholological assessment also showed that Al2O3 nanoparticles (250, 500, and 1000 µg/ml) lead to significant reduction of trabeculae. Empty lacunae are observed in these three groups. Considering that high concentrations of Al2O3 nanoparticles had toxicity on bone tissue, it must be used by more caution, especially its use as a coating in different devices such as implants, surgical instruments, and bone prostheses.http://dx.doi.org/10.1155/2020/8870530 |
| spellingShingle | Hossein Soltaninejad Hadi Zare-Zardini Amir Ali Hamidieh Mohammad Reza Sobhan Seyed Houssein Saeed-Banadaky Mohammad Amir Amirkhani Behnaz Tolueinia Mohsen Mehregan Mahnaz Mirakhor Farzaneh sadat Eshaghi Evaluating the Toxicity and Histological Effects of Al2O3 Nanoparticles on Bone Tissue in Animal Model: A Case-Control Study Journal of Toxicology |
| title | Evaluating the Toxicity and Histological Effects of Al2O3 Nanoparticles on Bone Tissue in Animal Model: A Case-Control Study |
| title_full | Evaluating the Toxicity and Histological Effects of Al2O3 Nanoparticles on Bone Tissue in Animal Model: A Case-Control Study |
| title_fullStr | Evaluating the Toxicity and Histological Effects of Al2O3 Nanoparticles on Bone Tissue in Animal Model: A Case-Control Study |
| title_full_unstemmed | Evaluating the Toxicity and Histological Effects of Al2O3 Nanoparticles on Bone Tissue in Animal Model: A Case-Control Study |
| title_short | Evaluating the Toxicity and Histological Effects of Al2O3 Nanoparticles on Bone Tissue in Animal Model: A Case-Control Study |
| title_sort | evaluating the toxicity and histological effects of al2o3 nanoparticles on bone tissue in animal model a case control study |
| url | http://dx.doi.org/10.1155/2020/8870530 |
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