Cryo-Imaging and Software Platform for Analysis of Molecular MR Imaging of Micrometastases
We created and evaluated a preclinical, multimodality imaging, and software platform to assess molecular imaging of small metastases. This included experimental methods (e.g., GFP-labeled tumor and high resolution multispectral cryo-imaging), nonrigid image registration, and interactive visualizatio...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Biomedical Imaging |
| Online Access: | http://dx.doi.org/10.1155/2018/9780349 |
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| author | Mohammed Q. Qutaish Zhuxian Zhou David Prabhu Yiqiao Liu Mallory R. Busso Donna Izadnegahdar Madhusudhana Gargesha Hong Lu Zheng-Rong Lu David L. Wilson |
| author_facet | Mohammed Q. Qutaish Zhuxian Zhou David Prabhu Yiqiao Liu Mallory R. Busso Donna Izadnegahdar Madhusudhana Gargesha Hong Lu Zheng-Rong Lu David L. Wilson |
| author_sort | Mohammed Q. Qutaish |
| collection | DOAJ |
| description | We created and evaluated a preclinical, multimodality imaging, and software platform to assess molecular imaging of small metastases. This included experimental methods (e.g., GFP-labeled tumor and high resolution multispectral cryo-imaging), nonrigid image registration, and interactive visualization of imaging agent targeting. We describe technological details earlier applied to GFP-labeled metastatic tumor targeting by molecular MR (CREKA-Gd) and red fluorescent (CREKA-Cy5) imaging agents. Optimized nonrigid cryo-MRI registration enabled nonambiguous association of MR signals to GFP tumors. Interactive visualization of out-of-RAM volumetric image data allowed one to zoom to a GFP-labeled micrometastasis, determine its anatomical location from color cryo-images, and establish the presence/absence of targeted CREKA-Gd and CREKA-Cy5. In a mouse with >160 GFP-labeled tumors, we determined that in the MR images every tumor in the lung >0.3 mm2 had visible signal and that some metastases as small as 0.1 mm2 were also visible. More tumors were visible in CREKA-Cy5 than in CREKA-Gd MRI. Tape transfer method and nonrigid registration allowed accurate (<11 μm error) registration of whole mouse histology to corresponding cryo-images. Histology showed inflammation and necrotic regions not labeled by imaging agents. This mouse-to-cells multiscale and multimodality platform should uniquely enable more informative and accurate studies of metastatic cancer imaging and therapy. |
| format | Article |
| id | doaj-art-9750f71159d8468a996f2846bd897974 |
| institution | Kabale University |
| issn | 1687-4188 1687-4196 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomedical Imaging |
| spelling | doaj-art-9750f71159d8468a996f2846bd8979742025-02-03T06:01:43ZengWileyInternational Journal of Biomedical Imaging1687-41881687-41962018-01-01201810.1155/2018/97803499780349Cryo-Imaging and Software Platform for Analysis of Molecular MR Imaging of MicrometastasesMohammed Q. Qutaish0Zhuxian Zhou1David Prabhu2Yiqiao Liu3Mallory R. Busso4Donna Izadnegahdar5Madhusudhana Gargesha6Hong Lu7Zheng-Rong Lu8David L. Wilson9Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USADepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USADepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USADepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USADepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USADepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USABioInVision Inc., Suite E, 781 Beta Drive, Cleveland, OH 44143, USADepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USADepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USADepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USAWe created and evaluated a preclinical, multimodality imaging, and software platform to assess molecular imaging of small metastases. This included experimental methods (e.g., GFP-labeled tumor and high resolution multispectral cryo-imaging), nonrigid image registration, and interactive visualization of imaging agent targeting. We describe technological details earlier applied to GFP-labeled metastatic tumor targeting by molecular MR (CREKA-Gd) and red fluorescent (CREKA-Cy5) imaging agents. Optimized nonrigid cryo-MRI registration enabled nonambiguous association of MR signals to GFP tumors. Interactive visualization of out-of-RAM volumetric image data allowed one to zoom to a GFP-labeled micrometastasis, determine its anatomical location from color cryo-images, and establish the presence/absence of targeted CREKA-Gd and CREKA-Cy5. In a mouse with >160 GFP-labeled tumors, we determined that in the MR images every tumor in the lung >0.3 mm2 had visible signal and that some metastases as small as 0.1 mm2 were also visible. More tumors were visible in CREKA-Cy5 than in CREKA-Gd MRI. Tape transfer method and nonrigid registration allowed accurate (<11 μm error) registration of whole mouse histology to corresponding cryo-images. Histology showed inflammation and necrotic regions not labeled by imaging agents. This mouse-to-cells multiscale and multimodality platform should uniquely enable more informative and accurate studies of metastatic cancer imaging and therapy.http://dx.doi.org/10.1155/2018/9780349 |
| spellingShingle | Mohammed Q. Qutaish Zhuxian Zhou David Prabhu Yiqiao Liu Mallory R. Busso Donna Izadnegahdar Madhusudhana Gargesha Hong Lu Zheng-Rong Lu David L. Wilson Cryo-Imaging and Software Platform for Analysis of Molecular MR Imaging of Micrometastases International Journal of Biomedical Imaging |
| title | Cryo-Imaging and Software Platform for Analysis of Molecular MR Imaging of Micrometastases |
| title_full | Cryo-Imaging and Software Platform for Analysis of Molecular MR Imaging of Micrometastases |
| title_fullStr | Cryo-Imaging and Software Platform for Analysis of Molecular MR Imaging of Micrometastases |
| title_full_unstemmed | Cryo-Imaging and Software Platform for Analysis of Molecular MR Imaging of Micrometastases |
| title_short | Cryo-Imaging and Software Platform for Analysis of Molecular MR Imaging of Micrometastases |
| title_sort | cryo imaging and software platform for analysis of molecular mr imaging of micrometastases |
| url | http://dx.doi.org/10.1155/2018/9780349 |
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