Decreased nitric oxide production is a novel therapeutic target for salt-induced nocturnal polyuria in aging
Abstract Nocturnal polyuria is a major cause of nocturia, which affects quality of life. Aging-related decreases in nitric oxide production have been reported to contribute to salt-induced nocturnal polyuria. We posited that enhanced nitric oxide production from exercise could mitigate salt-induced...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-86526-6 |
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| author | Takahiro Imanaka Kentaro Takezawa Yuma Kujime Hiroaki Kitakaze Go Tsujimura Sohei Kuribayashi Yosuke Sekii Norichika Ueda Shinichiro Fukuhara Hiroshi Kiuchi Hiromitsu Negoro Norio Nonomura |
| author_facet | Takahiro Imanaka Kentaro Takezawa Yuma Kujime Hiroaki Kitakaze Go Tsujimura Sohei Kuribayashi Yosuke Sekii Norichika Ueda Shinichiro Fukuhara Hiroshi Kiuchi Hiromitsu Negoro Norio Nonomura |
| author_sort | Takahiro Imanaka |
| collection | DOAJ |
| description | Abstract Nocturnal polyuria is a major cause of nocturia, which affects quality of life. Aging-related decreases in nitric oxide production have been reported to contribute to salt-induced nocturnal polyuria. We posited that enhanced nitric oxide production from exercise could mitigate salt-induced nocturnal polyuria. To validate, we assessed the impact of 4 weeks of moderate-intensity exercise on intrarenal renin-angiotensin system activity and salt-induced polyuria during inactive periods in 80-week-old mice. The moderate-intensity exercise group (Mod-Ex) had increased nitric oxide production and suppressed intrarenal renin-angiotensin system compared to the sedentary group (Sed) (renal angiotensinogen level: Sed vs. Mod-Ex, 1.101 ± 0.077 vs. 0.630 ± 0.048 arbitrary unit, P = 0.002). This exercise also alleviated salt-induced polyuria during inactive periods (diurnal polyuria index: Sed vs. Mod-Ex, 0.292 ± 0.027 vs. 0.193 ± 0.023, P = 0.018). These effects were neutralized by suppression of nitric oxide production by oral administration of Nω-Nitro-L-arginine methyl ester hydrochloride, an nitric oxide synthesis inhibitor. These findings indicate that exercise-induced improvements in salt-induced polyuria during inactive periods are caused by increases in nitric oxide production. In summary, increasing nitric oxide production may serve as a treatment method for salt-induced nocturnal polyuria in older adults. |
| format | Article |
| id | doaj-art-973f3e776dcd48bfba741097b9dc9268 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-973f3e776dcd48bfba741097b9dc92682025-02-02T12:23:21ZengNature PortfolioScientific Reports2045-23222025-01-011511910.1038/s41598-025-86526-6Decreased nitric oxide production is a novel therapeutic target for salt-induced nocturnal polyuria in agingTakahiro Imanaka0Kentaro Takezawa1Yuma Kujime2Hiroaki Kitakaze3Go Tsujimura4Sohei Kuribayashi5Yosuke Sekii6Norichika Ueda7Shinichiro Fukuhara8Hiroshi Kiuchi9Hiromitsu Negoro10Norio Nonomura11Department of Urology, Osaka University Graduate School of MedicineDepartment of Urology, Osaka University Graduate School of MedicineDepartment of Urology, Osaka University Graduate School of MedicineDepartment of Urology, Osaka University Graduate School of MedicineDepartment of Urology, Osaka University Graduate School of MedicineDepartment of Urology, Osaka University Graduate School of MedicineDepartment of Urology, Osaka University Graduate School of MedicineDepartment of Urology, Osaka University Graduate School of MedicineDepartment of Urology, Osaka University Graduate School of MedicineDepartment of Urology, Osaka University Graduate School of MedicineDepartment of Urology, Institute of Medicine, University of TsukubaDepartment of Urology, Osaka University Graduate School of MedicineAbstract Nocturnal polyuria is a major cause of nocturia, which affects quality of life. Aging-related decreases in nitric oxide production have been reported to contribute to salt-induced nocturnal polyuria. We posited that enhanced nitric oxide production from exercise could mitigate salt-induced nocturnal polyuria. To validate, we assessed the impact of 4 weeks of moderate-intensity exercise on intrarenal renin-angiotensin system activity and salt-induced polyuria during inactive periods in 80-week-old mice. The moderate-intensity exercise group (Mod-Ex) had increased nitric oxide production and suppressed intrarenal renin-angiotensin system compared to the sedentary group (Sed) (renal angiotensinogen level: Sed vs. Mod-Ex, 1.101 ± 0.077 vs. 0.630 ± 0.048 arbitrary unit, P = 0.002). This exercise also alleviated salt-induced polyuria during inactive periods (diurnal polyuria index: Sed vs. Mod-Ex, 0.292 ± 0.027 vs. 0.193 ± 0.023, P = 0.018). These effects were neutralized by suppression of nitric oxide production by oral administration of Nω-Nitro-L-arginine methyl ester hydrochloride, an nitric oxide synthesis inhibitor. These findings indicate that exercise-induced improvements in salt-induced polyuria during inactive periods are caused by increases in nitric oxide production. In summary, increasing nitric oxide production may serve as a treatment method for salt-induced nocturnal polyuria in older adults.https://doi.org/10.1038/s41598-025-86526-6Salt-induced nocturnal polyuriaNitric oxideIntrarenal renin-angiotensin systemAgingModerate-intensity exercise |
| spellingShingle | Takahiro Imanaka Kentaro Takezawa Yuma Kujime Hiroaki Kitakaze Go Tsujimura Sohei Kuribayashi Yosuke Sekii Norichika Ueda Shinichiro Fukuhara Hiroshi Kiuchi Hiromitsu Negoro Norio Nonomura Decreased nitric oxide production is a novel therapeutic target for salt-induced nocturnal polyuria in aging Scientific Reports Salt-induced nocturnal polyuria Nitric oxide Intrarenal renin-angiotensin system Aging Moderate-intensity exercise |
| title | Decreased nitric oxide production is a novel therapeutic target for salt-induced nocturnal polyuria in aging |
| title_full | Decreased nitric oxide production is a novel therapeutic target for salt-induced nocturnal polyuria in aging |
| title_fullStr | Decreased nitric oxide production is a novel therapeutic target for salt-induced nocturnal polyuria in aging |
| title_full_unstemmed | Decreased nitric oxide production is a novel therapeutic target for salt-induced nocturnal polyuria in aging |
| title_short | Decreased nitric oxide production is a novel therapeutic target for salt-induced nocturnal polyuria in aging |
| title_sort | decreased nitric oxide production is a novel therapeutic target for salt induced nocturnal polyuria in aging |
| topic | Salt-induced nocturnal polyuria Nitric oxide Intrarenal renin-angiotensin system Aging Moderate-intensity exercise |
| url | https://doi.org/10.1038/s41598-025-86526-6 |
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