Multiplex Bead Array Assay for Detection of 25 Soluble Cytokines in Blister Fluid of Patients with Complex Regional Pain Syndrome Type 1
Inflammatory processes are known to be involved at least in the early phase of complex regional pain syndrome type 1 (CRPS1). Blister fluid obtained from the involved extremities displayed increased amounts of proinflammatory cytokines IL-6 and TNFα compared with the noninvolved extremities. The ai...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2006-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/28398 |
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| Summary: | Inflammatory processes are known to be involved at least in the
early phase of complex regional pain syndrome type 1 (CRPS1).
Blister fluid obtained from the involved extremities displayed
increased amounts of proinflammatory cytokines IL-6 and TNFα
compared with the noninvolved extremities. The aim of this paper
is to investigate the involvement of mediators by measurement of
several other cytokines using new detection techniques that enable
multiple cytokine measurement in small samples. The use of a
multiplex-25 bead array cytokine assay and Luminex technology
enabled simultaneous measurement of representative (1)
proinflammatory cytokines such as GM-CSF, IL-1β, IL-1RA,
IL-6, IL-8, and TNF-α; (2) Th1/Th2 distinguishing
cytokines IFN-γ, IL-2, IL-2R, IL-4, IL-5, and IL-10; (3)
nonspecific acting cytokines IFN-α, IL-7, IL-12p40/p70,
IL-13, IL-15, and IL-17; and (4) chemokines eotaxin, IP-10, MCP-1,
MIP-1α, MIP-1β, MIG, and RANTES. Although minimal
detection levels are significantly higher in the bead array system
than those in common ELISA assays, in blister fluid, IL-1RA, IL-6,
IL-8, TNF-α, IL-12p40/p70, MCP-1, and MIP-1β were
detectable and increased in CRPS1 affected extremities. Levels of
IL-6 and TNF-α simultaneously measured by ELISA (Sanquin
Compact kit) and by multiplex-25 bead array assay (Biosource) were
highly correlated (r=0.85, P<.001
for IL-6 and r=0.88,
P<.001 for TNF-α). Furthermore, IP-10 and eotaxin were
detectable but diminished in CRPS1, whereas detectable amounts of
IL-10 were similar in involved and noninvolved extremities.
Multiplex bead array assays are useful systems to establish the
involvement of cytokines in inflammatory processes by measurements
in blister fluids of CRPS1. Ten representative cytokines were
detectable. However, detection levels and amounts measured are at
least 3 times higher in the multiplex-25 array assay than in the
ELISA assays used simultaneously for the measurement of cytokines. |
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| ISSN: | 0962-9351 1466-1861 |