Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells
Endomorphins (EMs) have a very important bridge-function in cardiovascular, endocrinological, and neurological systems. This study is to investigate the effects of EMs on the synthesis and secretion of vasoactive substances induced by advanced glycation end products in primary cultured human umbilic...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2013/105780 |
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| _version_ | 1832550709964308480 |
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| author | Jing Liu Liping Yan Ruilan Niu Limin Tian Qi Zhang Jinxing Quan Hua Liu Suhong Wei Qian Guo |
| author_facet | Jing Liu Liping Yan Ruilan Niu Limin Tian Qi Zhang Jinxing Quan Hua Liu Suhong Wei Qian Guo |
| author_sort | Jing Liu |
| collection | DOAJ |
| description | Endomorphins (EMs) have a very important bridge-function in
cardiovascular, endocrinological, and neurological systems. This
study is to investigate the effects of EMs on the synthesis and
secretion of vasoactive substances induced by advanced glycation
end products in primary cultured human umbilical vein endothelial
cells (HUVECs). Firstly, HUVECs were stimulated with AGEs-bovine
serum albumin (AGEs-BSA), bovine serum albumin (BSA), or both
AGEs-BSA and EMs together, respectively. Then, HUVEC survival rate
was calculated by MTT assay, the levels of NO, endothelial nitric
oxide synthase (eNOS), and inducible nitric oxide synthase (iNOS)
were detected by colorimetric analysis, and the contents of
endothelin-1 (ET-1) were detected by ELISA. The mRNA levels of
eNOS and ET-1 were measured by RT-PCR. The expression of p38
mitogen-activated protein kinase (p38 MAPK) was detected by
immunofluorescence assay. The results showed that the mRNA
expression and secretion of eNOS were significantly enhanced after
incubation with EMs compared to those with AGEs-BSA, while the
secretion of NO and iNOS, mRNA expression, and secretion of ET-1
had opposite changes. The fluorescence intensity of p38MAPK in
nuclear was decreased after pretreatment with EMs compared to
incubation with AGEs-BSA. Conclusion. The present
study suggests that EMs have certain protection effect on
AGEs-BSA-induced injury in HUVEC. |
| format | Article |
| id | doaj-art-96ab28e8438f42ef9f657bb9d893d2bb |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-96ab28e8438f42ef9f657bb9d893d2bb2025-02-03T06:06:05ZengWileyJournal of Diabetes Research2314-67452314-67532013-01-01201310.1155/2013/105780105780Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial CellsJing Liu0Liping Yan1Ruilan Niu2Limin Tian3Qi Zhang4Jinxing Quan5Hua Liu6Suhong Wei7Qian Guo8Department of Endocrinology, Gansu Provincial People’s Hospital, 204 West Donggang Road, Lanzhou City 730000, Gansu Province, ChinaDepartment of Endocrinology, Gansu Provincial People’s Hospital, 204 West Donggang Road, Lanzhou City 730000, Gansu Province, ChinaThe First Clinical College of Lanzhou University, Lanzhou City 730000, Gansu Province, ChinaDepartment of Endocrinology, Gansu Provincial People’s Hospital, 204 West Donggang Road, Lanzhou City 730000, Gansu Province, ChinaDepartment of Endocrinology, Gansu Provincial People’s Hospital, 204 West Donggang Road, Lanzhou City 730000, Gansu Province, ChinaDepartment of Endocrinology, Gansu Provincial People’s Hospital, 204 West Donggang Road, Lanzhou City 730000, Gansu Province, ChinaDepartment of Pneumology, Gansu Provincial People’s Hospital, 204 West Donggang Road, Lanzhou City 730000, Gansu Province, ChinaDepartment of Endocrinology, Gansu Provincial People’s Hospital, 204 West Donggang Road, Lanzhou City 730000, Gansu Province, ChinaDepartment of Endocrinology, Gansu Provincial People’s Hospital, 204 West Donggang Road, Lanzhou City 730000, Gansu Province, ChinaEndomorphins (EMs) have a very important bridge-function in cardiovascular, endocrinological, and neurological systems. This study is to investigate the effects of EMs on the synthesis and secretion of vasoactive substances induced by advanced glycation end products in primary cultured human umbilical vein endothelial cells (HUVECs). Firstly, HUVECs were stimulated with AGEs-bovine serum albumin (AGEs-BSA), bovine serum albumin (BSA), or both AGEs-BSA and EMs together, respectively. Then, HUVEC survival rate was calculated by MTT assay, the levels of NO, endothelial nitric oxide synthase (eNOS), and inducible nitric oxide synthase (iNOS) were detected by colorimetric analysis, and the contents of endothelin-1 (ET-1) were detected by ELISA. The mRNA levels of eNOS and ET-1 were measured by RT-PCR. The expression of p38 mitogen-activated protein kinase (p38 MAPK) was detected by immunofluorescence assay. The results showed that the mRNA expression and secretion of eNOS were significantly enhanced after incubation with EMs compared to those with AGEs-BSA, while the secretion of NO and iNOS, mRNA expression, and secretion of ET-1 had opposite changes. The fluorescence intensity of p38MAPK in nuclear was decreased after pretreatment with EMs compared to incubation with AGEs-BSA. Conclusion. The present study suggests that EMs have certain protection effect on AGEs-BSA-induced injury in HUVEC.http://dx.doi.org/10.1155/2013/105780 |
| spellingShingle | Jing Liu Liping Yan Ruilan Niu Limin Tian Qi Zhang Jinxing Quan Hua Liu Suhong Wei Qian Guo Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells Journal of Diabetes Research |
| title | Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells |
| title_full | Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells |
| title_fullStr | Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells |
| title_full_unstemmed | Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells |
| title_short | Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells |
| title_sort | protection effect of endomorphins on advanced glycation end products induced injury in endothelial cells |
| url | http://dx.doi.org/10.1155/2013/105780 |
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