Aptamer-Conjugated Multi-Quantum Dot-Embedded Silica Nanoparticles for Lateral Flow Immunoassay

Aptamer-Conjugated Multi-Quantum Dot-Embedded Silica Nanoparticles for Lateral Flow Immunoassay

Lateral flow immunoassays (LFIAs) are widely used for their low cost, simplicity, and rapid results; however, enhancing their reliability requires the meticulous selection of ligands and nanoparticles (NPs). SiO<sub>2</sub>@QD@SiO<sub>2</sub> (QD<sup>2</sup>) nano...

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Main Authors: Kwanghee Yoo, Hye-Seong Cho, Jaehi Kim, Minsup Shin, Jun-Sik Chu, Sohyeon Jang, Han-Joo Bae, Heung Su Jung, Homan Kang, Bong-Hyun Jun
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Biosensors
Subjects:
carbohydrate antigen 19-9
aptamer
quantum dot
lateral flow immunoassay
Online Access:https://www.mdpi.com/2079-6374/15/1/54
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Summary:Lateral flow immunoassays (LFIAs) are widely used for their low cost, simplicity, and rapid results; however, enhancing their reliability requires the meticulous selection of ligands and nanoparticles (NPs). SiO<sub>2</sub>@QD@SiO<sub>2</sub> (QD<sup>2</sup>) nanoparticles, which consist of quantum dots (QDs) embedded in a silica (SiO<sub>2</sub>) core and surrounded by an outer SiO<sub>2</sub> shell, exhibit significantly higher fluorescence intensity (FI) compared to single QDs. In this study, we prepared QD<sup>2</sup>@PEG@Aptamer, an aptamer conjugated with QD<sup>2</sup> using succinimidyl-[(N-maleimidopropionamido)-hexaethyleneglycol]ester, which is 130 times brighter than single QDs, for detecting carbohydrate antigen (CA) 19-9 through LFIA. For LFIA optimization, we determined the optimal conditions as a 1.0:2.0 × 10<sup>−2</sup> ratio of polyethylene glycol (PEG) to aptamer by adjusting the amounts of PEG and aptamer, phosphate-buffered saline containing 0.5% Tween<sup>®</sup> 20 as a developing solution, and 0.15 μg NPs by setting the NP weight during development. Under these conditions, QD<sup>2</sup>@PEG@Aptamer selectively detected CA19-9, achieving a detection limit of 1.74 × 10<sup>−2</sup> mg·mL<sup>−1</sup>. Moreover, FI remained stable for 10 days after detection. These results highlight the potential of QD<sup>2</sup> and aptamer conjugation technology as a reliable and versatile sensing platform for various diagnostic applications.
ISSN:2079-6374

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