Identification of Host–Protein Interaction Network of Canine Parvovirus Capsid Protein VP2 in F81 Cells
Canine Parvovirus (CPV) is a highly contagious virus that causes severe hemorrhagic enteritis and myocarditis, posing a major threat to the life and health of dogs. The molecular mechanism by which VP2, the major capsid protein of CPV, infects host cells and utilizes host cell proteins for self-repl...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/1/88 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587867556151296 |
|---|---|
| author | Hongzhuan Zhou Huanhuan Zhang Xia Su Fuzhou Xu Bing Xiao Jin Zhang Qi Qi Lulu Lin Kaidi Cui Qinqin Li Songping Li Bing Yang |
| author_facet | Hongzhuan Zhou Huanhuan Zhang Xia Su Fuzhou Xu Bing Xiao Jin Zhang Qi Qi Lulu Lin Kaidi Cui Qinqin Li Songping Li Bing Yang |
| author_sort | Hongzhuan Zhou |
| collection | DOAJ |
| description | Canine Parvovirus (CPV) is a highly contagious virus that causes severe hemorrhagic enteritis and myocarditis, posing a major threat to the life and health of dogs. The molecular mechanism by which VP2, the major capsid protein of CPV, infects host cells and utilizes host cell proteins for self-replication remains poorly understood. In this study, 140 host proteins specifically binding to CPV VP2 protein were identified by immunoprecipitation combined with liquid chromatography–mass spectrometry (LC-MS/MS). Subsequently, the protein Interaction Network (PPI), the annotation of gene ontology (GO) and the database of Kyoto Encyclopedia of Genes and Genomes (KEGG) were constructed for in-depth analysis. The results showed that CPV VP2 protein participated mainly in cell metabolism, cell biosynthesis, protein folding and various signal transduction processes. According to the results of proteomics analysis, we randomly selected seven proteins for co-immunoprecipitation verification, and the experimental results were consistent with the LC-MS/MS data. In addition, our study found that the expression level of the VP2-interacting protein FHL2 mediated CPV replication. Preliminary studies have shown that knockdown of FHL2 promotes CPV replication by decreasing the expression of interferon β (IFN-β) and interferon-stimulated genes (ISGs), while overexpression of FHL2 can inhibit the replication of CPV by up-regulating the expression of IFN-β and related ISGs. This study lays the foundation for elucidating the potential function of CPV VP2 protein in the process of viral infection and proliferation which provides a theoretical basis for the design of antiviral agents and vaccines. |
| format | Article |
| id | doaj-art-94ea8b29d133450bbbc92c24b8fb0bbf |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-94ea8b29d133450bbbc92c24b8fb0bbf2025-01-24T13:42:36ZengMDPI AGMicroorganisms2076-26072025-01-011318810.3390/microorganisms13010088Identification of Host–Protein Interaction Network of Canine Parvovirus Capsid Protein VP2 in F81 CellsHongzhuan Zhou0Huanhuan Zhang1Xia Su2Fuzhou Xu3Bing Xiao4Jin Zhang5Qi Qi6Lulu Lin7Kaidi Cui8Qinqin Li9Songping Li10Bing Yang11Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, In-Stitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, In-Stitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, In-Stitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, In-Stitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, In-Stitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, In-Stitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, In-Stitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, In-Stitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, In-Stitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, In-Stitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, In-Stitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, In-Stitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaCanine Parvovirus (CPV) is a highly contagious virus that causes severe hemorrhagic enteritis and myocarditis, posing a major threat to the life and health of dogs. The molecular mechanism by which VP2, the major capsid protein of CPV, infects host cells and utilizes host cell proteins for self-replication remains poorly understood. In this study, 140 host proteins specifically binding to CPV VP2 protein were identified by immunoprecipitation combined with liquid chromatography–mass spectrometry (LC-MS/MS). Subsequently, the protein Interaction Network (PPI), the annotation of gene ontology (GO) and the database of Kyoto Encyclopedia of Genes and Genomes (KEGG) were constructed for in-depth analysis. The results showed that CPV VP2 protein participated mainly in cell metabolism, cell biosynthesis, protein folding and various signal transduction processes. According to the results of proteomics analysis, we randomly selected seven proteins for co-immunoprecipitation verification, and the experimental results were consistent with the LC-MS/MS data. In addition, our study found that the expression level of the VP2-interacting protein FHL2 mediated CPV replication. Preliminary studies have shown that knockdown of FHL2 promotes CPV replication by decreasing the expression of interferon β (IFN-β) and interferon-stimulated genes (ISGs), while overexpression of FHL2 can inhibit the replication of CPV by up-regulating the expression of IFN-β and related ISGs. This study lays the foundation for elucidating the potential function of CPV VP2 protein in the process of viral infection and proliferation which provides a theoretical basis for the design of antiviral agents and vaccines.https://www.mdpi.com/2076-2607/13/1/88CPVVP2protein interaction networkFHL2 |
| spellingShingle | Hongzhuan Zhou Huanhuan Zhang Xia Su Fuzhou Xu Bing Xiao Jin Zhang Qi Qi Lulu Lin Kaidi Cui Qinqin Li Songping Li Bing Yang Identification of Host–Protein Interaction Network of Canine Parvovirus Capsid Protein VP2 in F81 Cells Microorganisms CPV VP2 protein interaction network FHL2 |
| title | Identification of Host–Protein Interaction Network of Canine Parvovirus Capsid Protein VP2 in F81 Cells |
| title_full | Identification of Host–Protein Interaction Network of Canine Parvovirus Capsid Protein VP2 in F81 Cells |
| title_fullStr | Identification of Host–Protein Interaction Network of Canine Parvovirus Capsid Protein VP2 in F81 Cells |
| title_full_unstemmed | Identification of Host–Protein Interaction Network of Canine Parvovirus Capsid Protein VP2 in F81 Cells |
| title_short | Identification of Host–Protein Interaction Network of Canine Parvovirus Capsid Protein VP2 in F81 Cells |
| title_sort | identification of host protein interaction network of canine parvovirus capsid protein vp2 in f81 cells |
| topic | CPV VP2 protein interaction network FHL2 |
| url | https://www.mdpi.com/2076-2607/13/1/88 |
| work_keys_str_mv | AT hongzhuanzhou identificationofhostproteininteractionnetworkofcanineparvoviruscapsidproteinvp2inf81cells AT huanhuanzhang identificationofhostproteininteractionnetworkofcanineparvoviruscapsidproteinvp2inf81cells AT xiasu identificationofhostproteininteractionnetworkofcanineparvoviruscapsidproteinvp2inf81cells AT fuzhouxu identificationofhostproteininteractionnetworkofcanineparvoviruscapsidproteinvp2inf81cells AT bingxiao identificationofhostproteininteractionnetworkofcanineparvoviruscapsidproteinvp2inf81cells AT jinzhang identificationofhostproteininteractionnetworkofcanineparvoviruscapsidproteinvp2inf81cells AT qiqi identificationofhostproteininteractionnetworkofcanineparvoviruscapsidproteinvp2inf81cells AT lululin identificationofhostproteininteractionnetworkofcanineparvoviruscapsidproteinvp2inf81cells AT kaidicui identificationofhostproteininteractionnetworkofcanineparvoviruscapsidproteinvp2inf81cells AT qinqinli identificationofhostproteininteractionnetworkofcanineparvoviruscapsidproteinvp2inf81cells AT songpingli identificationofhostproteininteractionnetworkofcanineparvoviruscapsidproteinvp2inf81cells AT bingyang identificationofhostproteininteractionnetworkofcanineparvoviruscapsidproteinvp2inf81cells |