Chemical Differentiation and Quantitative Analysis of Different Types of Panax Genus Stem-Leaf Based on a UPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis Approach
Two quantitative methods (−ESI full scan and −ESI PRM MS) were developed to analyze ginsenosides in ginseng stem-leaf by using UPLC-Q-Exactive Orbitrap/MS. By means of −ESI PRM MS method, the contents of eighteen ginsenosides in Asian ginseng stem-leaf (ASGSL) and American ginseng stem-leaf (AMGSL)...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/9598672 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564278842884096 |
|---|---|
| author | Lele Li Yang Wang Yang Xiu Shuying Liu |
| author_facet | Lele Li Yang Wang Yang Xiu Shuying Liu |
| author_sort | Lele Li |
| collection | DOAJ |
| description | Two quantitative methods (−ESI full scan and −ESI PRM MS) were developed to analyze ginsenosides in ginseng stem-leaf by using UPLC-Q-Exactive Orbitrap/MS. By means of −ESI PRM MS method, the contents of eighteen ginsenosides in Asian ginseng stem-leaf (ASGSL) and American ginseng stem-leaf (AMGSL) were analyzed. The principal component analysis (PCA) model was built to discriminate Asian ginseng stem-leaf (ASGSL) from American ginseng stem-leaf (AMGSL) based on −ESI PRM MS data, and six ginsenosides (F11, Rf, R2, F1, Rb1, and Rb3) were obtained as the markers. To further explore the differences between cultivated ginseng stem-leaf and forest ginseng stem-leaf, the partial least squares-discriminant analysis (PLS-DA) model was built based on −ESI full scan data. And twenty-six markers were selected to discriminate cultivated ginseng stem-leaf (CGSL) from forest ginseng stem-leaf (FGSL). This study provides reliable and effective methods to quantify and discriminate among different types of ginseng stem-leaf in the commercial market. |
| format | Article |
| id | doaj-art-94c0e1865b9146e0b0cd8ec78ae5b08d |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-94c0e1865b9146e0b0cd8ec78ae5b08d2025-02-03T01:11:26ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/95986729598672Chemical Differentiation and Quantitative Analysis of Different Types of Panax Genus Stem-Leaf Based on a UPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis ApproachLele Li0Yang Wang1Yang Xiu2Shuying Liu3Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, ChinaJilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, ChinaJilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, ChinaJilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, ChinaTwo quantitative methods (−ESI full scan and −ESI PRM MS) were developed to analyze ginsenosides in ginseng stem-leaf by using UPLC-Q-Exactive Orbitrap/MS. By means of −ESI PRM MS method, the contents of eighteen ginsenosides in Asian ginseng stem-leaf (ASGSL) and American ginseng stem-leaf (AMGSL) were analyzed. The principal component analysis (PCA) model was built to discriminate Asian ginseng stem-leaf (ASGSL) from American ginseng stem-leaf (AMGSL) based on −ESI PRM MS data, and six ginsenosides (F11, Rf, R2, F1, Rb1, and Rb3) were obtained as the markers. To further explore the differences between cultivated ginseng stem-leaf and forest ginseng stem-leaf, the partial least squares-discriminant analysis (PLS-DA) model was built based on −ESI full scan data. And twenty-six markers were selected to discriminate cultivated ginseng stem-leaf (CGSL) from forest ginseng stem-leaf (FGSL). This study provides reliable and effective methods to quantify and discriminate among different types of ginseng stem-leaf in the commercial market.http://dx.doi.org/10.1155/2018/9598672 |
| spellingShingle | Lele Li Yang Wang Yang Xiu Shuying Liu Chemical Differentiation and Quantitative Analysis of Different Types of Panax Genus Stem-Leaf Based on a UPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis Approach Journal of Analytical Methods in Chemistry |
| title | Chemical Differentiation and Quantitative Analysis of Different Types of Panax Genus Stem-Leaf Based on a UPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis Approach |
| title_full | Chemical Differentiation and Quantitative Analysis of Different Types of Panax Genus Stem-Leaf Based on a UPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis Approach |
| title_fullStr | Chemical Differentiation and Quantitative Analysis of Different Types of Panax Genus Stem-Leaf Based on a UPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis Approach |
| title_full_unstemmed | Chemical Differentiation and Quantitative Analysis of Different Types of Panax Genus Stem-Leaf Based on a UPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis Approach |
| title_short | Chemical Differentiation and Quantitative Analysis of Different Types of Panax Genus Stem-Leaf Based on a UPLC-Q-Exactive Orbitrap/MS Combined with Multivariate Statistical Analysis Approach |
| title_sort | chemical differentiation and quantitative analysis of different types of panax genus stem leaf based on a uplc q exactive orbitrap ms combined with multivariate statistical analysis approach |
| url | http://dx.doi.org/10.1155/2018/9598672 |
| work_keys_str_mv | AT leleli chemicaldifferentiationandquantitativeanalysisofdifferenttypesofpanaxgenusstemleafbasedonauplcqexactiveorbitrapmscombinedwithmultivariatestatisticalanalysisapproach AT yangwang chemicaldifferentiationandquantitativeanalysisofdifferenttypesofpanaxgenusstemleafbasedonauplcqexactiveorbitrapmscombinedwithmultivariatestatisticalanalysisapproach AT yangxiu chemicaldifferentiationandquantitativeanalysisofdifferenttypesofpanaxgenusstemleafbasedonauplcqexactiveorbitrapmscombinedwithmultivariatestatisticalanalysisapproach AT shuyingliu chemicaldifferentiationandquantitativeanalysisofdifferenttypesofpanaxgenusstemleafbasedonauplcqexactiveorbitrapmscombinedwithmultivariatestatisticalanalysisapproach |