Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes
Transcription factor Lbx1 is known to play a role in the migration of muscle progenitor cells in limb buds and also in neuronal determination processes. In addition, involvement of Lbx1 in cardiac neural crest-related cardiogenesis was postulated. Here, we used...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.4061/2011/130970 |
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| _version_ | 1832549114865254400 |
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| author | Stefanie Schmitteckert Cornelia Ziegler Liane Kartes Alexandra Rolletschek |
| author_facet | Stefanie Schmitteckert Cornelia Ziegler Liane Kartes Alexandra Rolletschek |
| author_sort | Stefanie Schmitteckert |
| collection | DOAJ |
| description | Transcription factor Lbx1 is known to play a role
in the migration of muscle progenitor cells in limb
buds and also in neuronal determination processes. In
addition, involvement of Lbx1 in cardiac neural crest-related cardiogenesis was postulated. Here, we used
mouse embryonic stem (ES) cells which have the
capacity to develop into cells of all three primary
germ layers. During in vitro
differentiation, ES cells recapitulate cellular
developmental processes and gene expression patterns
of early embryogenesis. Transcript analysis revealed a
significant upregulation of Lbx1 at
the progenitor cell stage. Immunofluorescence staining
confirmed the expression of Lbx1 in skeletal muscle
cell progenitors and GABAergic neurons. To verify the
presence of Lbx1 in cardiac cells, triple
immunocytochemistry of ES cell-derived cardiomyocytes
and a quantification assay were performed at different
developmental stages. Colabeling of Lbx1 and cardiac
specific markers troponin T, α-actinin, GATA4,
and Nkx2.5 suggested a potential role in early
myocardial development. |
| format | Article |
| id | doaj-art-94a230e830a24e1d990810f48e01b4f7 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-94a230e830a24e1d990810f48e01b4f72025-02-03T06:12:16ZengWileyStem Cells International1687-966X1687-96782011-01-01201110.4061/2011/130970130970Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived PhenotypesStefanie Schmitteckert0Cornelia Ziegler1Liane Kartes2Alexandra Rolletschek3Institute for Biological Interfaces 1, Karlsruhe Institute of Technology (KIT) Campus North, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, GermanyInstitute for Biological Interfaces 1, Karlsruhe Institute of Technology (KIT) Campus North, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, GermanyInstitute for Biological Interfaces 1, Karlsruhe Institute of Technology (KIT) Campus North, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, GermanyInstitute for Biological Interfaces 1, Karlsruhe Institute of Technology (KIT) Campus North, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, GermanyTranscription factor Lbx1 is known to play a role in the migration of muscle progenitor cells in limb buds and also in neuronal determination processes. In addition, involvement of Lbx1 in cardiac neural crest-related cardiogenesis was postulated. Here, we used mouse embryonic stem (ES) cells which have the capacity to develop into cells of all three primary germ layers. During in vitro differentiation, ES cells recapitulate cellular developmental processes and gene expression patterns of early embryogenesis. Transcript analysis revealed a significant upregulation of Lbx1 at the progenitor cell stage. Immunofluorescence staining confirmed the expression of Lbx1 in skeletal muscle cell progenitors and GABAergic neurons. To verify the presence of Lbx1 in cardiac cells, triple immunocytochemistry of ES cell-derived cardiomyocytes and a quantification assay were performed at different developmental stages. Colabeling of Lbx1 and cardiac specific markers troponin T, α-actinin, GATA4, and Nkx2.5 suggested a potential role in early myocardial development.http://dx.doi.org/10.4061/2011/130970 |
| spellingShingle | Stefanie Schmitteckert Cornelia Ziegler Liane Kartes Alexandra Rolletschek Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes Stem Cells International |
| title | Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes |
| title_full | Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes |
| title_fullStr | Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes |
| title_full_unstemmed | Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes |
| title_short | Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes |
| title_sort | transcription factor lbx1 expression in mouse embryonic stem cell derived phenotypes |
| url | http://dx.doi.org/10.4061/2011/130970 |
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