Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes
Transcription factor Lbx1 is known to play a role in the migration of muscle progenitor cells in limb buds and also in neuronal determination processes. In addition, involvement of Lbx1 in cardiac neural crest-related cardiogenesis was postulated. Here, we used...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.4061/2011/130970 |
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| Summary: | Transcription factor Lbx1 is known to play a role
in the migration of muscle progenitor cells in limb
buds and also in neuronal determination processes. In
addition, involvement of Lbx1 in cardiac neural crest-related cardiogenesis was postulated. Here, we used
mouse embryonic stem (ES) cells which have the
capacity to develop into cells of all three primary
germ layers. During in vitro
differentiation, ES cells recapitulate cellular
developmental processes and gene expression patterns
of early embryogenesis. Transcript analysis revealed a
significant upregulation of Lbx1 at
the progenitor cell stage. Immunofluorescence staining
confirmed the expression of Lbx1 in skeletal muscle
cell progenitors and GABAergic neurons. To verify the
presence of Lbx1 in cardiac cells, triple
immunocytochemistry of ES cell-derived cardiomyocytes
and a quantification assay were performed at different
developmental stages. Colabeling of Lbx1 and cardiac
specific markers troponin T, α-actinin, GATA4,
and Nkx2.5 suggested a potential role in early
myocardial development. |
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| ISSN: | 1687-966X 1687-9678 |