Effect of Dalmavital and Ovulik Plus on conception rate in Ovsynch synchronized Nili-Ravi buf- faloes

Nili-Ravi buffalo are known as the “Black Gold of Pakistan”. It is the most prized dairy animal in Pakistan. Silent estrus and shy behavior are among the main causes of poor reproductive efficiency in buffalo, leading to prolonged calving intervals. Ovulik Plus® is a supplementary mixture for stimu...

Full description

Saved in:
Bibliographic Details
Main Authors: Muhammad Tayyab Khan, Muhammad Muzammal Niaz, Ghulam Shabbir Khan
Format: Article
Language:English
Published: Universidad del Zulia 2023-11-01
Series:Revista Científica
Subjects:
Online Access:https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43446
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832542836351827968
author Muhammad Tayyab Khan
Muhammad Muzammal Niaz
Ghulam Shabbir Khan
author_facet Muhammad Tayyab Khan
Muhammad Muzammal Niaz
Ghulam Shabbir Khan
author_sort Muhammad Tayyab Khan
collection DOAJ
description Nili-Ravi buffalo are known as the “Black Gold of Pakistan”. It is the most prized dairy animal in Pakistan. Silent estrus and shy behavior are among the main causes of poor reproductive efficiency in buffalo, leading to prolonged calving intervals. Ovulik Plus® is a supplementary mixture for stimulating fertility. It provides energy, eliminates the deficiency of ß-carotene, as well as giving a rich source of vitamins and microelements to elevate reproductive efficiency. Dalmavital® is another product that contains ß-carotene and acts as an antioxidant source. Our objective was to compare the impact of these supplements in enhancing conception rates of Ovsynch synchronized Nili-Ravi buffaloes. For this purpose, buffaloes (n=45) with healthy reproductive tract, BCS (2.5-3.5), and mixed parity (2-3) were enrolled into three groups: G1: Ovsynch + Dalmavital (IM), G2: Ovsynch + Ovulik (PO) and G3: Ovsynch without supplementation as control. For the Ovsynch protocol, buffaloes were administered with 2ml (IM) injection of GnRH (Lecirelin acetate 25μg/ml) at day 0, followed by 2ml (IM) injection of PGF2α (D-cloprostenol 75μg/ml) at day 7. Finally, 2ml GnRH was injected (IM) at day 9. Dalmavital was injected (IM) at the rate of 1ml/40kg body weight at day 5 in G1, and Ovulik Plus was offered by drenching gun just after 1st AI as a single dose of 1000 g/ buffalo. Double inseminations were performed at standing estrus, morning and evening, at intervals of 12 and 24 h after the last GnRH injection. Pregnancies were confirmed through ultrasonography at day 40. Data were analyzed through the Chi-Square test and Binary Logistic Regression model using SPSS. Results revealed no difference (p>0.05) in estrus response among all groups. Conception rates were higher (p<0.05) in G2 compared to G3 (60% vs. 46.66%, respectively). However, there was  no difference (p >0.05) in conception rates of G1 and G2 (53.33% vs. 60%, respectively). In conclusion, Ovulik Plus per oral was superior to intramuscular administration of Dalmavital in improving conception rates of Ovsynch synchronized Nili-Ravi buffaloes.
format Article
id doaj-art-9360869802d14061b96b2cf37f15e9c9
institution Kabale University
issn 0798-2259
2521-9715
language English
publishDate 2023-11-01
publisher Universidad del Zulia
record_format Article
series Revista Científica
spelling doaj-art-9360869802d14061b96b2cf37f15e9c92025-02-03T15:37:01ZengUniversidad del ZuliaRevista Científica0798-22592521-97152023-11-0133Suplemento10.52973/rcfcv-wbc102Effect of Dalmavital and Ovulik Plus on conception rate in Ovsynch synchronized Nili-Ravi buf- faloesMuhammad Tayyab Khan0Muhammad Muzammal Niaz 1Ghulam Shabbir Khan2Department of Theriogenology, University of Veterinary and Animal Sciences, outfall Road, Lahore (54000), PakistanDepartment of Theriogenology, University of Veterinary and Animal Sciences, outfall Road, Lahore (54000), PakistanPMAS Arid Agriculture University Rawalpindi, Sub Campus Khushab. Pakistan Nili-Ravi buffalo are known as the “Black Gold of Pakistan”. It is the most prized dairy animal in Pakistan. Silent estrus and shy behavior are among the main causes of poor reproductive efficiency in buffalo, leading to prolonged calving intervals. Ovulik Plus® is a supplementary mixture for stimulating fertility. It provides energy, eliminates the deficiency of ß-carotene, as well as giving a rich source of vitamins and microelements to elevate reproductive efficiency. Dalmavital® is another product that contains ß-carotene and acts as an antioxidant source. Our objective was to compare the impact of these supplements in enhancing conception rates of Ovsynch synchronized Nili-Ravi buffaloes. For this purpose, buffaloes (n=45) with healthy reproductive tract, BCS (2.5-3.5), and mixed parity (2-3) were enrolled into three groups: G1: Ovsynch + Dalmavital (IM), G2: Ovsynch + Ovulik (PO) and G3: Ovsynch without supplementation as control. For the Ovsynch protocol, buffaloes were administered with 2ml (IM) injection of GnRH (Lecirelin acetate 25μg/ml) at day 0, followed by 2ml (IM) injection of PGF2α (D-cloprostenol 75μg/ml) at day 7. Finally, 2ml GnRH was injected (IM) at day 9. Dalmavital was injected (IM) at the rate of 1ml/40kg body weight at day 5 in G1, and Ovulik Plus was offered by drenching gun just after 1st AI as a single dose of 1000 g/ buffalo. Double inseminations were performed at standing estrus, morning and evening, at intervals of 12 and 24 h after the last GnRH injection. Pregnancies were confirmed through ultrasonography at day 40. Data were analyzed through the Chi-Square test and Binary Logistic Regression model using SPSS. Results revealed no difference (p>0.05) in estrus response among all groups. Conception rates were higher (p<0.05) in G2 compared to G3 (60% vs. 46.66%, respectively). However, there was  no difference (p >0.05) in conception rates of G1 and G2 (53.33% vs. 60%, respectively). In conclusion, Ovulik Plus per oral was superior to intramuscular administration of Dalmavital in improving conception rates of Ovsynch synchronized Nili-Ravi buffaloes. https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43446Ovulik PlusDalmavitalß-caroteneOvsynchNili-Ravi Buffalo
spellingShingle Muhammad Tayyab Khan
Muhammad Muzammal Niaz
Ghulam Shabbir Khan
Effect of Dalmavital and Ovulik Plus on conception rate in Ovsynch synchronized Nili-Ravi buf- faloes
Revista Científica
Ovulik Plus
Dalmavital
ß-carotene
Ovsynch
Nili-Ravi Buffalo
title Effect of Dalmavital and Ovulik Plus on conception rate in Ovsynch synchronized Nili-Ravi buf- faloes
title_full Effect of Dalmavital and Ovulik Plus on conception rate in Ovsynch synchronized Nili-Ravi buf- faloes
title_fullStr Effect of Dalmavital and Ovulik Plus on conception rate in Ovsynch synchronized Nili-Ravi buf- faloes
title_full_unstemmed Effect of Dalmavital and Ovulik Plus on conception rate in Ovsynch synchronized Nili-Ravi buf- faloes
title_short Effect of Dalmavital and Ovulik Plus on conception rate in Ovsynch synchronized Nili-Ravi buf- faloes
title_sort effect of dalmavital and ovulik plus on conception rate in ovsynch synchronized nili ravi buf faloes
topic Ovulik Plus
Dalmavital
ß-carotene
Ovsynch
Nili-Ravi Buffalo
url https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43446
work_keys_str_mv AT muhammadtayyabkhan effectofdalmavitalandovulikplusonconceptionrateinovsynchsynchronizedniliravibuffaloes
AT muhammadmuzammalniaz effectofdalmavitalandovulikplusonconceptionrateinovsynchsynchronizedniliravibuffaloes
AT ghulamshabbirkhan effectofdalmavitalandovulikplusonconceptionrateinovsynchsynchronizedniliravibuffaloes