The Effect of Autologous Platelet-Rich Gel on the Dynamic Changes of the Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 Expression in the Diabetic Chronic Refractory Cutaneous Ulcers
Aim. To investigate the dynamic changes on the expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in the diabetic chronic refractory cutaneous ulcers after the autologous platelet-rich gel (APG) treatment. Methods. The study was developed at the Diabeti...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2015/954701 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832545727355551744 |
|---|---|
| author | Lan Li Dawei Chen Chun Wang Guanjian Liu Xingwu Ran |
| author_facet | Lan Li Dawei Chen Chun Wang Guanjian Liu Xingwu Ran |
| author_sort | Lan Li |
| collection | DOAJ |
| description | Aim. To investigate the dynamic changes on the expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in the diabetic chronic refractory cutaneous ulcers after the autologous platelet-rich gel (APG) treatment. Methods. The study was developed at the Diabetic Foot Care Centre, West China Hospital. The granulation tissues from the target wounds were taken before and within 15 days after APG application. The expression of MMP-2 and TIMP-2 as well as transforming growth factor-β1 (TGF-β1) in the granulation tissue was detected by q TR-PCR and IHC. The relationship between the expression level of MMP-2 and TIMP-2 and their ratio and that of TGF-β1 was analyzed. Results. The expression of MMP-2 (P < 0.05) was suppressed, and the expression of TIMP-2 (P < 0.05) was promoted, while the ratio of MMP-2/TIMP-2 (P < 0.05) was decreased after APG treatments. The expression of TGF-β1 had negative correlation with the ratio of MMP-2/TIMP-2 (P < 0.05) and positive correlation with the expression of TIMP-2 (P < 0.05). Conclusions. APG treatment may suppress the expression of MMP-2, promoting that of the TIMP-2 in the diabetic chronic refractory cutaneous wounds. TGF-β1 may be related to these effects. |
| format | Article |
| id | doaj-art-9252e7ca9eb24d0f9c76eb21f59467cd |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-9252e7ca9eb24d0f9c76eb21f59467cd2025-02-03T07:24:58ZengWileyJournal of Diabetes Research2314-67452314-67532015-01-01201510.1155/2015/954701954701The Effect of Autologous Platelet-Rich Gel on the Dynamic Changes of the Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 Expression in the Diabetic Chronic Refractory Cutaneous UlcersLan Li0Dawei Chen1Chun Wang2Guanjian Liu3Xingwu Ran4Diabetic Foot Care Center, Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu 610041, ChinaDiabetic Foot Care Center, Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu 610041, ChinaDiabetic Foot Care Center, Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu 610041, ChinaChinese Cochrane Centre, Chinese EBM Centre, West China Hospital, Sichuan University, Chengdu 610041, ChinaDiabetic Foot Care Center, Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu 610041, ChinaAim. To investigate the dynamic changes on the expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in the diabetic chronic refractory cutaneous ulcers after the autologous platelet-rich gel (APG) treatment. Methods. The study was developed at the Diabetic Foot Care Centre, West China Hospital. The granulation tissues from the target wounds were taken before and within 15 days after APG application. The expression of MMP-2 and TIMP-2 as well as transforming growth factor-β1 (TGF-β1) in the granulation tissue was detected by q TR-PCR and IHC. The relationship between the expression level of MMP-2 and TIMP-2 and their ratio and that of TGF-β1 was analyzed. Results. The expression of MMP-2 (P < 0.05) was suppressed, and the expression of TIMP-2 (P < 0.05) was promoted, while the ratio of MMP-2/TIMP-2 (P < 0.05) was decreased after APG treatments. The expression of TGF-β1 had negative correlation with the ratio of MMP-2/TIMP-2 (P < 0.05) and positive correlation with the expression of TIMP-2 (P < 0.05). Conclusions. APG treatment may suppress the expression of MMP-2, promoting that of the TIMP-2 in the diabetic chronic refractory cutaneous wounds. TGF-β1 may be related to these effects.http://dx.doi.org/10.1155/2015/954701 |
| spellingShingle | Lan Li Dawei Chen Chun Wang Guanjian Liu Xingwu Ran The Effect of Autologous Platelet-Rich Gel on the Dynamic Changes of the Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 Expression in the Diabetic Chronic Refractory Cutaneous Ulcers Journal of Diabetes Research |
| title | The Effect of Autologous Platelet-Rich Gel on the Dynamic Changes of the Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 Expression in the Diabetic Chronic Refractory Cutaneous Ulcers |
| title_full | The Effect of Autologous Platelet-Rich Gel on the Dynamic Changes of the Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 Expression in the Diabetic Chronic Refractory Cutaneous Ulcers |
| title_fullStr | The Effect of Autologous Platelet-Rich Gel on the Dynamic Changes of the Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 Expression in the Diabetic Chronic Refractory Cutaneous Ulcers |
| title_full_unstemmed | The Effect of Autologous Platelet-Rich Gel on the Dynamic Changes of the Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 Expression in the Diabetic Chronic Refractory Cutaneous Ulcers |
| title_short | The Effect of Autologous Platelet-Rich Gel on the Dynamic Changes of the Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 Expression in the Diabetic Chronic Refractory Cutaneous Ulcers |
| title_sort | effect of autologous platelet rich gel on the dynamic changes of the matrix metalloproteinase 2 and tissue inhibitor of metalloproteinase 2 expression in the diabetic chronic refractory cutaneous ulcers |
| url | http://dx.doi.org/10.1155/2015/954701 |
| work_keys_str_mv | AT lanli theeffectofautologousplateletrichgelonthedynamicchangesofthematrixmetalloproteinase2andtissueinhibitorofmetalloproteinase2expressioninthediabeticchronicrefractorycutaneousulcers AT daweichen theeffectofautologousplateletrichgelonthedynamicchangesofthematrixmetalloproteinase2andtissueinhibitorofmetalloproteinase2expressioninthediabeticchronicrefractorycutaneousulcers AT chunwang theeffectofautologousplateletrichgelonthedynamicchangesofthematrixmetalloproteinase2andtissueinhibitorofmetalloproteinase2expressioninthediabeticchronicrefractorycutaneousulcers AT guanjianliu theeffectofautologousplateletrichgelonthedynamicchangesofthematrixmetalloproteinase2andtissueinhibitorofmetalloproteinase2expressioninthediabeticchronicrefractorycutaneousulcers AT xingwuran theeffectofautologousplateletrichgelonthedynamicchangesofthematrixmetalloproteinase2andtissueinhibitorofmetalloproteinase2expressioninthediabeticchronicrefractorycutaneousulcers AT lanli effectofautologousplateletrichgelonthedynamicchangesofthematrixmetalloproteinase2andtissueinhibitorofmetalloproteinase2expressioninthediabeticchronicrefractorycutaneousulcers AT daweichen effectofautologousplateletrichgelonthedynamicchangesofthematrixmetalloproteinase2andtissueinhibitorofmetalloproteinase2expressioninthediabeticchronicrefractorycutaneousulcers AT chunwang effectofautologousplateletrichgelonthedynamicchangesofthematrixmetalloproteinase2andtissueinhibitorofmetalloproteinase2expressioninthediabeticchronicrefractorycutaneousulcers AT guanjianliu effectofautologousplateletrichgelonthedynamicchangesofthematrixmetalloproteinase2andtissueinhibitorofmetalloproteinase2expressioninthediabeticchronicrefractorycutaneousulcers AT xingwuran effectofautologousplateletrichgelonthedynamicchangesofthematrixmetalloproteinase2andtissueinhibitorofmetalloproteinase2expressioninthediabeticchronicrefractorycutaneousulcers |