Estimation of ribosomal RNA transcription rate in situ

Traditionally, the rate of transcription is measured by metabolic labeling (e.g., the run-on assay), which can be carried out only in isolated or cultured cells. It has been difficult if not impossible to assess the rate of transcription of a gene in a specific cell type in situ. We show here that t...

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Bibliographic Details
Main Authors: Chunhua Cui, Hung Tseng
Format: Article
Language:English
Published: Taylor & Francis Group 2004-01-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/04361RR04
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