Characteristics of the smallest brucellaphage with strong lytic ability
Brucellosis is a globally prevalent zoonotic disease caused by Brucella spp. posing significant threats to animal and human health. In this study, a novel lytic brucellaphage designated Y17 was isolated from sheep fecal samples collected in Ludian County, Yunnan Province, China. Transmission electro...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1530123/full |
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| author | Hongbaiyu Liu Hongbaiyu Liu Youhong Zhong Zhihong Zhang Kehong Xu Kehong Xu Chunpeng Mao Chunpeng Mao Qiuju Yang Lihua Yang Binbin Yu Ying Long Ying Long Xinyu Qin Xinyu Qin Liyuan Shi Sheng Chang Yuanying Shen Peng Wang |
| author_facet | Hongbaiyu Liu Hongbaiyu Liu Youhong Zhong Zhihong Zhang Kehong Xu Kehong Xu Chunpeng Mao Chunpeng Mao Qiuju Yang Lihua Yang Binbin Yu Ying Long Ying Long Xinyu Qin Xinyu Qin Liyuan Shi Sheng Chang Yuanying Shen Peng Wang |
| author_sort | Hongbaiyu Liu |
| collection | DOAJ |
| description | Brucellosis is a globally prevalent zoonotic disease caused by Brucella spp. posing significant threats to animal and human health. In this study, a novel lytic brucellaphage designated Y17 was isolated from sheep fecal samples collected in Ludian County, Yunnan Province, China. Transmission electron microscopy revealed that Y17 was composed of an icosahedral head (48.1 ± 2 nm) and a short tail (10.8 ± 1 nm), making it the smallest brucellaphage described so far. The optimal multiplicity of infection (MOI) for phage Y17 is 0.001, with a burst size of ~187 PFU/cell, the largest value reported for any brucellaphage, and it has a relatively short latent period. It exhibits broad pH and temperature stability, retaining activity even after 1 h of exposure to ultraviolet radiation and various ethanol concentrations. Y17 shows strong lytic activity against Brucella abortus and can also infect some Brucella melitensis strains. The Y17 genome spans 38,025 bp with a GC content of 48.2%, making it the smallest genome among brucellaphages to date. It lacks virulence, antibiotic resistance, or lysogenic genes, indicating its potential as a safe biocontrol agent. Whole-genome average nucleotide identity (ANI) analysis reveals high homology across all lytic brucellaphages, but Y17 exhibits relatively lower genome coverage compared to other lytic brucellaphages. Genomic collinearity comparison revealed that Y17 lacks some terminal fragments present in the genomes of other lytic brucellaphages. Furthermore, compared to brucellaphages with genomes larger than 40 kb, Y17 also lacks segments corresponding to ORF21 (amidase), ORF28 (hypothetical protein), and ORF29 (carbohydrate-binding protein). Phylogenetic analysis indicates that Y17 is closely related to phages Iz, Bk2, S708, Wb, R/C, Pr, and Bk. Moreover, the capsid gene shows significantly higher conservation in comparison with the tail collar and amidase genes. This study significantly enriches the brucellaphage database and highlights the potential of Y17 as a biocontrol agent for managing brucellosis in endemic regions. |
| format | Article |
| id | doaj-art-921a3433afac4c3fab4007c1179eec07 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj-art-921a3433afac4c3fab4007c1179eec072025-02-05T11:18:32ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-02-011210.3389/fvets.2025.15301231530123Characteristics of the smallest brucellaphage with strong lytic abilityHongbaiyu Liu0Hongbaiyu Liu1Youhong Zhong2Zhihong Zhang3Kehong Xu4Kehong Xu5Chunpeng Mao6Chunpeng Mao7Qiuju Yang8Lihua Yang9Binbin Yu10Ying Long11Ying Long12Xinyu Qin13Xinyu Qin14Liyuan Shi15Sheng Chang16Yuanying Shen17Peng Wang18Department of Medical Microbiology and Immunology, School of Basic Medical Sciences, Dali University, Dali, ChinaYunnan Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali, ChinaYunnan Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali, ChinaChuxiong Center for Disease Control and Prevention, Chuxiong, ChinaYunnan Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali, ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical, College of Pharmacy, Dali University, Dali, ChinaYunnan Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali, ChinaSchool of Public Health, Kunming Medical University, Kunming, ChinaYunnan Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali, ChinaYunnan Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali, ChinaYunnan Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali, ChinaDepartment of Medical Microbiology and Immunology, School of Basic Medical Sciences, Dali University, Dali, ChinaYunnan Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali, ChinaYunnan Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali, ChinaSchool of Public Health, Dali University, Dali, ChinaYunnan Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali, ChinaSchool of Public Health, Kunming Medical University, Kunming, ChinaDepartment of Medical Microbiology and Immunology, School of Basic Medical Sciences, Dali University, Dali, ChinaYunnan Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali, ChinaBrucellosis is a globally prevalent zoonotic disease caused by Brucella spp. posing significant threats to animal and human health. In this study, a novel lytic brucellaphage designated Y17 was isolated from sheep fecal samples collected in Ludian County, Yunnan Province, China. Transmission electron microscopy revealed that Y17 was composed of an icosahedral head (48.1 ± 2 nm) and a short tail (10.8 ± 1 nm), making it the smallest brucellaphage described so far. The optimal multiplicity of infection (MOI) for phage Y17 is 0.001, with a burst size of ~187 PFU/cell, the largest value reported for any brucellaphage, and it has a relatively short latent period. It exhibits broad pH and temperature stability, retaining activity even after 1 h of exposure to ultraviolet radiation and various ethanol concentrations. Y17 shows strong lytic activity against Brucella abortus and can also infect some Brucella melitensis strains. The Y17 genome spans 38,025 bp with a GC content of 48.2%, making it the smallest genome among brucellaphages to date. It lacks virulence, antibiotic resistance, or lysogenic genes, indicating its potential as a safe biocontrol agent. Whole-genome average nucleotide identity (ANI) analysis reveals high homology across all lytic brucellaphages, but Y17 exhibits relatively lower genome coverage compared to other lytic brucellaphages. Genomic collinearity comparison revealed that Y17 lacks some terminal fragments present in the genomes of other lytic brucellaphages. Furthermore, compared to brucellaphages with genomes larger than 40 kb, Y17 also lacks segments corresponding to ORF21 (amidase), ORF28 (hypothetical protein), and ORF29 (carbohydrate-binding protein). Phylogenetic analysis indicates that Y17 is closely related to phages Iz, Bk2, S708, Wb, R/C, Pr, and Bk. Moreover, the capsid gene shows significantly higher conservation in comparison with the tail collar and amidase genes. This study significantly enriches the brucellaphage database and highlights the potential of Y17 as a biocontrol agent for managing brucellosis in endemic regions.https://www.frontiersin.org/articles/10.3389/fvets.2025.1530123/fullphageBrucellabiological characteristicsgenome analysissmallest brucellaphage |
| spellingShingle | Hongbaiyu Liu Hongbaiyu Liu Youhong Zhong Zhihong Zhang Kehong Xu Kehong Xu Chunpeng Mao Chunpeng Mao Qiuju Yang Lihua Yang Binbin Yu Ying Long Ying Long Xinyu Qin Xinyu Qin Liyuan Shi Sheng Chang Yuanying Shen Peng Wang Characteristics of the smallest brucellaphage with strong lytic ability Frontiers in Veterinary Science phage Brucella biological characteristics genome analysis smallest brucellaphage |
| title | Characteristics of the smallest brucellaphage with strong lytic ability |
| title_full | Characteristics of the smallest brucellaphage with strong lytic ability |
| title_fullStr | Characteristics of the smallest brucellaphage with strong lytic ability |
| title_full_unstemmed | Characteristics of the smallest brucellaphage with strong lytic ability |
| title_short | Characteristics of the smallest brucellaphage with strong lytic ability |
| title_sort | characteristics of the smallest brucellaphage with strong lytic ability |
| topic | phage Brucella biological characteristics genome analysis smallest brucellaphage |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1530123/full |
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