Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction
Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport and the endocrine/paracrine ECL cells effectively control parietal cell function. Discontinuous gradients of iodixanol for the purification of ECL cells were subsequently simplified to the...
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| Format: | Article |
| Language: | English |
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Wiley
2002-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2002.852 |
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| _version_ | 1832548411553873920 |
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| author | John Graham |
| author_facet | John Graham |
| author_sort | John Graham |
| collection | DOAJ |
| description | Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport and the endocrine/paracrine ECL cells effectively control parietal cell function. Discontinuous gradients of iodixanol for the purification of ECL cells were subsequently simplified to the use of a density barrier. This technique is now commonly used following initial centrifugal elutriation. |
| format | Article |
| id | doaj-art-91835227ae724e568e7b68324d06bb77 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-91835227ae724e568e7b68324d06bb772025-02-03T06:14:12ZengWileyThe Scientific World Journal1537-744X2002-01-0121643164510.1100/tsw.2002.852Purification of Gastric Mucosal ECL Cells from a Crude Elutriation FractionJohn Graham0School of Biomolecular Sciences, Liverpool John Moores University, UKAcid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport and the endocrine/paracrine ECL cells effectively control parietal cell function. Discontinuous gradients of iodixanol for the purification of ECL cells were subsequently simplified to the use of a density barrier. This technique is now commonly used following initial centrifugal elutriation.http://dx.doi.org/10.1100/tsw.2002.852 |
| spellingShingle | John Graham Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction The Scientific World Journal |
| title | Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction |
| title_full | Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction |
| title_fullStr | Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction |
| title_full_unstemmed | Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction |
| title_short | Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction |
| title_sort | purification of gastric mucosal ecl cells from a crude elutriation fraction |
| url | http://dx.doi.org/10.1100/tsw.2002.852 |
| work_keys_str_mv | AT johngraham purificationofgastricmucosaleclcellsfromacrudeelutriationfraction |