Effects of laparoscopic sleeve gastrectomy on nonalcoholic fatty liver disease and TGF-β signaling pathway
Nonalcoholic fatty liver disease (NAFLD) develops as a result of unhealthy lifestyle but improves with laparoscopic sleeve gastrectomy (LSG). The transforming growth factor (TGF)-β signaling pathway reportedly contributes to liver fibrosis, mainly in animal experiments. The aim of the present study...
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| Format: | Article |
| Language: | English |
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The Japan Endocrine Society
2024-02-01
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| Series: | Endocrine Journal |
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| Online Access: | https://www.jstage.jst.go.jp/article/endocrj/71/2/71_EJ23-0411/_html/-char/en |
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| author | Hideki Kumagai Akira Sasaki Akira Umemura Keisuke Kakisaka Takeshi Iwaya Satoshi S Nishizuka |
| author_facet | Hideki Kumagai Akira Sasaki Akira Umemura Keisuke Kakisaka Takeshi Iwaya Satoshi S Nishizuka |
| author_sort | Hideki Kumagai |
| collection | DOAJ |
| description | Nonalcoholic fatty liver disease (NAFLD) develops as a result of unhealthy lifestyle but improves with laparoscopic sleeve gastrectomy (LSG). The transforming growth factor (TGF)-β signaling pathway reportedly contributes to liver fibrosis, mainly in animal experiments. The aim of the present study was to evaluate changes in serum proteins before and after LSG by proteomic analysis and to investigate their association with NAFLD. This study enrolled 36 severely obese patients who underwent LSG at our hospital from January 2020 to April 2022. As a pilot study, proteomic analysis was conducted on six patients using serum collected before and at 6 months after LSG, and significantly fluctuating proteins were extracted. Subsequently, verification by enzyme-linked immunosorbent assay (ELISA) using collected serum was performed on the remaining 30 patients. The mean weight of enrolled patients was 118.5 kg. Proteomic analysis identified 1,912 proteins, many of which were related to the TGF-β signaling pathway. Among these proteins, we focused on five TGF-β-related proteins: asporin, EMILIN-1, platelet factor-4, serglycin, and thrombospondin-1. Verification by ELISA revealed that asporin (p = 0.006) and thrombospondin-1 (p = 0.043) levels significantly fluctuated before and after LSG. Univariate analysis with a linear regression model showed that aspartate aminotransferase (p = 0.045), asporin (p = 0.011), and thrombospondin-1 (p = 0.022) levels were significantly associated with postoperative liver fibrosis. On multivariate analysis, asporin was an independent prognostic factor for postoperative liver fibrosis (95% confidence interval: 0.114–1.291, p = 0.002). TGF-β-related proteins dramatically fluctuated before and after LSG and were correlated with NAFLD pathogenesis. Asporin may be a useful prognostic marker of liver fibrosis in NAFLD after LSG. |
| format | Article |
| id | doaj-art-8f82b28f52a649cc8cf9b07259d3e955 |
| institution | Kabale University |
| issn | 1348-4540 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | The Japan Endocrine Society |
| record_format | Article |
| series | Endocrine Journal |
| spelling | doaj-art-8f82b28f52a649cc8cf9b07259d3e9552025-01-22T06:33:21ZengThe Japan Endocrine SocietyEndocrine Journal1348-45402024-02-0171213915210.1507/endocrj.EJ23-0411endocrjEffects of laparoscopic sleeve gastrectomy on nonalcoholic fatty liver disease and TGF-β signaling pathwayHideki Kumagai0Akira Sasaki1Akira Umemura2Keisuke Kakisaka3Takeshi Iwaya4Satoshi S Nishizuka5Department of Surgery, School of Medicine, Iwate Medical University, Iwate 028-3695, JapanDepartment of Surgery, School of Medicine, Iwate Medical University, Iwate 028-3695, JapanDepartment of Surgery, School of Medicine, Iwate Medical University, Iwate 028-3695, JapanDivision of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine, Iwate Medical University, Iwate 028-3695, JapanDepartment of Clinical Oncology, School of Medicine, Iwate Medical University, Iwate 028-3695, JapanDivision of Biomedical Research and Development, Iwate Medical University Institute for Biomedical Sciences, Iwate 028-3694, JapanNonalcoholic fatty liver disease (NAFLD) develops as a result of unhealthy lifestyle but improves with laparoscopic sleeve gastrectomy (LSG). The transforming growth factor (TGF)-β signaling pathway reportedly contributes to liver fibrosis, mainly in animal experiments. The aim of the present study was to evaluate changes in serum proteins before and after LSG by proteomic analysis and to investigate their association with NAFLD. This study enrolled 36 severely obese patients who underwent LSG at our hospital from January 2020 to April 2022. As a pilot study, proteomic analysis was conducted on six patients using serum collected before and at 6 months after LSG, and significantly fluctuating proteins were extracted. Subsequently, verification by enzyme-linked immunosorbent assay (ELISA) using collected serum was performed on the remaining 30 patients. The mean weight of enrolled patients was 118.5 kg. Proteomic analysis identified 1,912 proteins, many of which were related to the TGF-β signaling pathway. Among these proteins, we focused on five TGF-β-related proteins: asporin, EMILIN-1, platelet factor-4, serglycin, and thrombospondin-1. Verification by ELISA revealed that asporin (p = 0.006) and thrombospondin-1 (p = 0.043) levels significantly fluctuated before and after LSG. Univariate analysis with a linear regression model showed that aspartate aminotransferase (p = 0.045), asporin (p = 0.011), and thrombospondin-1 (p = 0.022) levels were significantly associated with postoperative liver fibrosis. On multivariate analysis, asporin was an independent prognostic factor for postoperative liver fibrosis (95% confidence interval: 0.114–1.291, p = 0.002). TGF-β-related proteins dramatically fluctuated before and after LSG and were correlated with NAFLD pathogenesis. Asporin may be a useful prognostic marker of liver fibrosis in NAFLD after LSG.https://www.jstage.jst.go.jp/article/endocrj/71/2/71_EJ23-0411/_html/-char/enlaparoscopic sleeve gastrectomy (lsg)transforming growth factor-β (tgf-β)asporin (aspn)nonalcoholic fatty liver disease (nafld)liver fibrosis |
| spellingShingle | Hideki Kumagai Akira Sasaki Akira Umemura Keisuke Kakisaka Takeshi Iwaya Satoshi S Nishizuka Effects of laparoscopic sleeve gastrectomy on nonalcoholic fatty liver disease and TGF-β signaling pathway Endocrine Journal laparoscopic sleeve gastrectomy (lsg) transforming growth factor-β (tgf-β) asporin (aspn) nonalcoholic fatty liver disease (nafld) liver fibrosis |
| title | Effects of laparoscopic sleeve gastrectomy on nonalcoholic fatty liver disease and TGF-β signaling pathway |
| title_full | Effects of laparoscopic sleeve gastrectomy on nonalcoholic fatty liver disease and TGF-β signaling pathway |
| title_fullStr | Effects of laparoscopic sleeve gastrectomy on nonalcoholic fatty liver disease and TGF-β signaling pathway |
| title_full_unstemmed | Effects of laparoscopic sleeve gastrectomy on nonalcoholic fatty liver disease and TGF-β signaling pathway |
| title_short | Effects of laparoscopic sleeve gastrectomy on nonalcoholic fatty liver disease and TGF-β signaling pathway |
| title_sort | effects of laparoscopic sleeve gastrectomy on nonalcoholic fatty liver disease and tgf β signaling pathway |
| topic | laparoscopic sleeve gastrectomy (lsg) transforming growth factor-β (tgf-β) asporin (aspn) nonalcoholic fatty liver disease (nafld) liver fibrosis |
| url | https://www.jstage.jst.go.jp/article/endocrj/71/2/71_EJ23-0411/_html/-char/en |
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